Sanghita Sarkar1, Ann J Hessell2, Nancy L Haigwood2, James J Kobie1. 1. Infectious Diseases Division, University of Alabama at Birmingham, Birmingham, AL, USA. 2. Oregon National Primate Research Center, Oregon Health & Science University, Beaverton, OR, USA.
Abstract
BACKGROUND: IL-9 and IL-33 can profoundly influence immune responses. As a necessary first step toward defining their impact in the rhesus macaque model, we confirmed their endogenous expression and sequence identity and generated expression vectors for the recombinant expression of rhesus IL-9 and IL-33. METHODS: RT-PCR and Sanger sequencing was used to define the expression and sequences for rhesus IL-9 and IL-33. The resulting recombinant cytokines were tested by ELISA and proliferation assays. RESULTS: Full-length rhesus IL-9 and the mature form of rhesus IL-33 share 78% and 73% nucleotide similarity, respectively, with humans. Both cytokines are expressed in lymphocytes, with IL-9 expression also evident in CD4+ T cells. Recombinantly expressed rhesus IL-9 and IL-33 were each biologically active in vitro, including enhancing the proliferation of a rhesus B cell line. CONCLUSIONS: The recombinant rhesus IL-9 and IL-33 constructs produce biologically active cytokines that can act upon rhesus B cells.
BACKGROUND:IL-9 and IL-33 can profoundly influence immune responses. As a necessary first step toward defining their impact in the rhesus macaque model, we confirmed their endogenous expression and sequence identity and generated expression vectors for the recombinant expression of rhesus IL-9 and IL-33. METHODS: RT-PCR and Sanger sequencing was used to define the expression and sequences for rhesus IL-9 and IL-33. The resulting recombinant cytokines were tested by ELISA and proliferation assays. RESULTS: Full-length rhesus IL-9 and the mature form of rhesus IL-33 share 78% and 73% nucleotide similarity, respectively, with humans. Both cytokines are expressed in lymphocytes, with IL-9expression also evident in CD4+ T cells. Recombinantly expressed rhesus IL-9 and IL-33 were each biologically active in vitro, including enhancing the proliferation of a rhesus B cell line. CONCLUSIONS: The recombinant rhesus IL-9 and IL-33 constructs produce biologically active cytokines that can act upon rhesus B cells.
Authors: Lama M Fawaz; Ehssan Sharif-Askari; Oumnia Hajoui; Abdelilah Soussi-Gounni; Qutayba Hamid; Bruce D Mazer Journal: J Allergy Clin Immunol Date: 2007-10-24 Impact factor: 10.793
Authors: Marc Veldhoen; Catherine Uyttenhove; Jacques van Snick; Helena Helmby; Astrid Westendorf; Jan Buer; Bruno Martin; Christoph Wilhelm; Brigitta Stockinger Journal: Nat Immunol Date: 2008-10-19 Impact factor: 25.606
Authors: Wassim Elyaman; Elizabeth M Bradshaw; Catherine Uyttenhove; Valérie Dardalhon; Amit Awasthi; Jaime Imitola; Estelle Bettelli; Mohamed Oukka; Jacques van Snick; Jean-Christophe Renauld; Vijay K Kuchroo; Samia J Khoury Journal: Proc Natl Acad Sci U S A Date: 2009-05-11 Impact factor: 11.205
Authors: William A Rose; Angela J Okragly; Ningjie N Hu; Montanea R Daniels; Andrea P Martin; Yi Ting Koh; Kristine Kikly; Robert J Benschop Journal: Front Immunol Date: 2018-12-06 Impact factor: 7.561