| Literature DB >> 32015505 |
Mattia Lorenzo DiFrancesco1,2, Francesco Lodola3, Elisabetta Colombo1,2, Luca Maragliano1,2, Mattia Bramini1,2,4, Giuseppe Maria Paternò3, Pietro Baldelli2,5, Mauro Dalla Serra6,7, Lorenzo Lunelli6,7, Marta Marchioretto6,7, Giorgio Grasselli1,2, Simone Cimò3,8, Letizia Colella8, Daniele Fazzi9, Fausto Ortica10, Vito Vurro3, Cyril Giles Eleftheriou1,2,11, Dmytro Shmal1,2, José Fernando Maya-Vetencourt1,2,12, Chiara Bertarelli3,8, Guglielmo Lanzani13, Fabio Benfenati14,15.
Abstract
Optical technologies allowing modulation of neuronal activity at high spatio-temporal resolution are becoming paramount in neuroscience. In this respect, azobenzene-based photoswitches are promising nanoscale tools for neuronal photostimulation. Here we engineered a light-sensitive azobenzene compound (Ziapin2) that stably partitions into the plasma membrane and causes its thinning through trans-dimerization in the dark, resulting in an increased membrane capacitance at steady state. We demonstrated that in neurons loaded with the compound, millisecond pulses of visible light induce a transient hyperpolarization followed by a delayed depolarization that triggers action potential firing. These effects are persistent and can be evoked in vivo up to 7 days, proving the potential of Ziapin2 for the modulation of membrane capacitance in the millisecond timescale, without directly affecting ion channels or local temperature.Entities:
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Year: 2020 PMID： 32015505 DOI： 10.1038/s41565-019-0632-6
Source DB: PubMed Journal: Nat Nanotechnol ISSN： 1748-3387 Impact factor: 39.213