Literature DB >> 32015001

Production of Recombinant Adeno-Associated Viruses (rAAVs) by Transient Transfection.

Qin Su, Miguel Sena-Esteves, Guangping Gao.   

Abstract

The most commonly used method for production of recombinant adeno-associated virus (rAAVs) in research laboratories is by transient triple transfection of 293 cells with AAV cis and trans plasmids and an adenovirus helper plasmid. This protocol describes the processes required to prepare the transfected cell suspension for virus purification.
© 2020 Cold Spring Harbor Laboratory Press.

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Year:  2020        PMID： 32015001     DOI： 10.1101/pdb.prot095596

Source DB:  PubMed          Journal:  Cold Spring Harb Protoc        ISSN： 1559-6095


  6 in total

1.  Secretion of functional α1-antitrypsin is cell type dependent: Implications for intramuscular delivery for gene therapy.

Authors:  Haiping Ke; Kevin P Guay; Terence R Flotte; Lila M Gierasch; Anne Gershenson; Daniel N Hebert
Journal:  Proc Natl Acad Sci U S A       Date:  2022-07-28       Impact factor: 12.779

2.  AAV5 delivery of CRISPR-Cas9 supports effective genome editing in mouse lung airway.

Authors:  Shun-Qing Liang; Christopher J Walkey; Alexa E Martinez; Qin Su; Mary E Dickinson; Dan Wang; William R Lagor; Jason D Heaney; Guangping Gao; Wen Xue
Journal:  Mol Ther       Date:  2021-10-23       Impact factor: 12.910

3.  Treatment Potential for LCA5-Associated Leber Congenital Amaurosis.

Authors:  Katherine E Uyhazi; Puya Aravand; Brent A Bell; Zhangyong Wei; Lanfranco Leo; Leona W Serrano; Denise J Pearson; Ivan Shpylchak; Jennifer Pham; Vidyullatha Vasireddy; Jean Bennett; Tomas S Aleman
Journal:  Invest Ophthalmol Vis Sci       Date:  2020-05-11       Impact factor: 4.799

4.  Novel Combinatorial MicroRNA-Binding Sites in AAV Vectors Synergistically Diminish Antigen Presentation and Transgene Immunity for Efficient and Stable Transduction.

Authors:  Manish Muhuri; Wei Zhan; Yukiko Maeda; Jia Li; Anoushka Lotun; Jennifer Chen; Katelyn Sylvia; Ishani Dasgupta; Motahareh Arjomandnejad; Thomas Nixon; Allison M Keeler; Sangeetha Manokaran; Ran He; Qin Su; Phillip W L Tai; Guangping Gao
Journal:  Front Immunol       Date:  2021-04-28       Impact factor: 8.786

5.  Stent-based delivery of AAV2 vectors encoding oxidation-resistant apoA1.

Authors:  Bahman Hooshdaran; Benjamin B Pressly; Ivan S Alferiev; Jonathan D Smith; Philip W Zoltick; Cory M Tschabrunn; Robert L Wilensky; Robert C Gorman; Robert J Levy; Ilia Fishbein
Journal:  Sci Rep       Date:  2022-03-31       Impact factor: 4.996

6.  rAAV-delivered PTEN therapeutics for prostate cancer.

Authors:  Jianzhong Ai; Jia Li; Qin Su; Hong Ma; Qiang Wei; Hong Li; Guangping Gao
Journal:  Mol Ther Nucleic Acids       Date:  2021-11-29       Impact factor: 10.183
  6 in total

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