Lyubomir Haralambiev1,2, Andreas Nitsch2, Rebekka Einenkel3, Damian O Muzzio3, Nadine Gelbrich4, Martin Burchardt4, Marek Zygmunt3, Axel Ekkernkamp5,2, Matthias B Stope4, Denis Gümbel5,2. 1. Department of Trauma, Reconstructive Surgery and Rehabilitation Medicine, University Medicine Greifswald, Greifswald, Germany lyubomir.haralambiev@uni-greifswald.de. 2. Department of Trauma and Orthopaedic Surgery, BG Klinikum Unfallkrankenhaus Berlin gGmbH, Berlin, Germany. 3. Department of Gynecology and Obstetrics, University Medicine Greifswald, Greifswald, Germany. 4. Department of Urology, University Medicine Greifswald, Greifswald, Germany. 5. Department of Trauma, Reconstructive Surgery and Rehabilitation Medicine, University Medicine Greifswald, Greifswald, Germany.
Abstract
BACKGROUND: Cold atmospheric plasma (CAP) has a variety of anticancer effects on different cancer cell types. In osteosarcoma (OS) cells, CAP reduces growth and motility, induces apoptosis, and alters secretion of cellular factors. The influence of CAP on membrane integrity of OS cells is unknown. MATERIALS AND METHODS: Two different OS cell lines (U-2 OS and MNNG-HOS) were treated with CAP. Proliferation assays for cell growth after treatment was performed. Alterations in membrane permeability and the associated translocation of low molecular weight particles through the cytoplasmic membrane of OS cells after CAP treatment were shown in fluorescein diacetate (FDA) assays. RESULTS: FDA increasingly passed the membrane after CAP treatment and this effect depended on the duration of treatment. It was also shown that after CAP treatment, FDA was able to diffuse into the cells from the outside as well as out of the cell interior. These effects were observed when CAP-treated buffer was used and therefore no direct contact between cells and CAP occurred. CONCLUSION: The observations suggest that changes in membrane permeability and function may contribute to the antiproliferative effects of CAP. Copyright
BACKGROUND:Cold atmospheric plasma (CAP) has a variety of anticancer effects on different cancer cell types. In osteosarcoma (OS) cells, CAP reduces growth and motility, induces apoptosis, and alters secretion of cellular factors. The influence of CAP on membrane integrity of OS cells is unknown. MATERIALS AND METHODS: Two different OS cell lines (U-2 OS and MNNG-HOS) were treated with CAP. Proliferation assays for cell growth after treatment was performed. Alterations in membrane permeability and the associated translocation of low molecular weight particles through the cytoplasmic membrane of OS cells after CAP treatment were shown in fluorescein diacetate (FDA) assays. RESULTS:FDA increasingly passed the membrane after CAP treatment and this effect depended on the duration of treatment. It was also shown that after CAP treatment, FDA was able to diffuse into the cells from the outside as well as out of the cell interior. These effects were observed when CAP-treated buffer was used and therefore no direct contact between cells and CAP occurred. CONCLUSION: The observations suggest that changes in membrane permeability and function may contribute to the antiproliferative effects of CAP. Copyright
Authors: Philipp Kugler; Sven Becker; Christian Welz; Nadine Wiesmann; Jonas Sax; Christoph R Buhr; Markus H Thoma; Juergen Brieger; Jonas Eckrich Journal: Cancers (Basel) Date: 2022-05-14 Impact factor: 6.575
Authors: Josephine M Jacoby; Silas Strakeljahn; Andreas Nitsch; Sander Bekeschus; Peter Hinz; Alexander Mustea; Axel Ekkernkamp; Mladen V Tzvetkov; Lyubomir Haralambiev; Matthias B Stope Journal: Int J Mol Sci Date: 2020-06-23 Impact factor: 5.923
Authors: Lyubomir Haralambiev; Andreas Nitsch; Josephine M Jacoby; Silas Strakeljahn; Sander Bekeschus; Alexander Mustea; Axel Ekkernkamp; Matthias B Stope Journal: Int J Mol Sci Date: 2020-03-26 Impact factor: 5.923
Authors: Andreas Nitsch; Silas Strakeljahn; Josephine M Jacoby; Konrad F Sieb; Alexander Mustea; Sander Bekeschus; Axel Ekkernkamp; Matthias B Stope; Lyubomir Haralambiev Journal: Biomedicines Date: 2022-03-17