Biodiversity of organisms inhabiting the water supply network of Wroclaw. Detection of pathogenic organisms constituting a threat for drinking water recipients.

The objective of the article was to present the diversity of organisms inhabiting the water supply network with particular consideration of pathogenic organisms that can cause an epidemiological threat, with the application of high throughput sequencing (HTS). The study material was water sampled from 15 points in the water supply system. High species diversity of bacteria was evidenced, as well as the presence of microorganisms from genus Clostridium and family Enterobacteriaceae. No presence of bacteria Clostridium perfringens was recorded, which suggests proper performance of water treatment processes. Owing to advanced techniques of molecular biology, the article also presents species very similar to pathogenic bacteria the detection of which is not possible by means of standard water analysis (plate culture). Based on literature data and very high similarity of the genome of the bacteria to that of pathogenic bacteria, the species are considered to potentially show the same negative character towards the recipient, and cause a serious epidemiological threat. Therefore, the performed analyses show that classic methods of assessment of sanitary quality of water are not fully sufficient, and HTS analysis should be performed as an auxiliary tool to provide the complete image of the community in the existing network.