| Literature DB >> 32013802 |
Silvina S Maidana1,2,3,4,5, Samuel Miño1,2,3,4,5, Romina M Apostolo1,2,3,4,5, Gabriel A De Stefano1,2,3,4,5, Sonia A Romera1,2,3,4,5.
Abstract
Bovine herpesvirus 1 (BoHV-1) causes several clinical syndromes in cattle worldwide. There are 3 subtypes of BoHV-1: 1.1, 1.2a, and 1.2b. Several molecular methods are commonly used in the detection and characterization of BoHV-1. Among them, restriction endonuclease analysis (REA) and single-nucleotide polymorphism (SNP) analysis of the complete viral genome allow classification of BoHV-1 into different subtypes. However, developing countries need simpler and cheaper screening assays for routine testing. We designed a standard multiplex PCR followed by a REA assay allowing straightforward subclassification of all BoHV-1 isolates tested into 1.1, 1.2a, and 1.2b subtypes based on the analysis of fragment length polymorphism. Our standard multiplex PCR-REA was used to analyze 33 field strains of BoHV-1 isolated from various tissues. The assay confirmed the subtype identified previously by REA. In addition, non-polymorphic or undigested fragments were sequenced in order to confirm the mutation affecting the RE HindIII site. Our PCR-REA method is an affordable and rapid test that will subtype all BoHV-1 strains.Entities:
Keywords: BoHV-1; BoHV-1 subtyping; multiplex PCR; restriction endonuclease analysis
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Year: 2020 PMID： 32013802 PMCID： PMC7003222 DOI： 10.1177/1040638719898692
Source DB: PubMed Journal: J Vet Diagn Invest ISSN： 1040-6387 Impact factor: 1.279