Literature DB >> 32011333

Author`s Reply.

Li Zhao1.   

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Year:  2020        PMID: 32011333      PMCID: PMC7040872     

Source DB:  PubMed          Journal:  Anatol J Cardiol        ISSN: 2149-2263            Impact factor:   1.596


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To the Editor, I appreciate his interest in my study (1). He has pointed out about the rationalization of the concentrations of trimetazidine (200 µM) and coenzyme Q10 (200 mg/L) used in the ventricular myocytes. I performed the preliminary experiments to analyze the responses of rat cardiomyocytes to serial doses of TMZ (12.5–200 µM) or CoQ10 (12.5–200 mg/L). TMZ or CoQ10 attenuated cisplatin-induced cell toxicity in a dose-dependent manner using a CCK8 assay. However, statistical significance was only observed at a concentration of 200 µM TMZ or 200 mg/L CoQ10. Therefore, I chose to use 200 µM TMZ and 200 mg/L CoQ10 for subsequent experiments. He has recommended measuring parameters, such as intracellular calcium levels and caspase 3 and caspase 9 activities. These parameters could be useful to investigate the mechanisms of the cardioprotective role of trimetazidine in chemotherapy-induced cardiotoxicity; however, my study focused on the upstream of caspase activities as described in the paper’s introduction. ROS-mitochondrial dysfunction-Nrf2/CytoC-apoptosis was the major framework of my study. On the other hand, caspase-dependent apoptosis has been briefly dealt with in the paper’s introduction and discussion.
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1.  Protective effects of trimetazidine and coenzyme Q10 on cisplatin-induced cardiotoxicity by alleviating oxidative stress and mitochondrial dysfunction.

Authors:  Li Zhao
Journal:  Anatol J Cardiol       Date:  2019-11       Impact factor: 1.596

  1 in total

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