Subur P Pasaribu1,2, Jamaran Kaban1, Mimpin Ginting1, Jansen Silalahi3. 1. Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan-20155, Indonesia. 2. Department of Chemistry, Faculty of Mathematics and Natural Sciences, Mulawarman University, Samarinda-75123, Indonesia. 3. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Sumatera Utara, Medan-20155, Indonesia.
Abstract
AIM: This study was aimed to prepare in situ cross-linked N-maleoyl chitosan - oxidised sodium alginate (MCS - OSA) hydrogel loaded with metronidazole (MTZ) for drug delivery applications. METHODS: The hydrogel was prepared by in situ cross-linking via Schiff base reaction between amine (-NH2) groups from MCS and aldehyde (-CHO) groups from OSA at the different ratio, and the MTZ was loaded into the hydrogels along with the gelatin processes. RESULTS: The highest drug entrapment efficiency (DEE) was exhibited by MTZ-H3 (5: 5) with DEE of 99.20% and a gel fraction of 97.52%. FTIR results revealed that Schiff base reaction was occurred by the absorption peak of -C = N- groups at 1628 cm-1 and indicated that there is insignificant alteration at different ratio of MCS and OSA. The best sustained of in vitro release profiles of MTZ was shown by MTZ-H3, which is 74.92% and 75.65% at pH 1.2 and 7.4 for 12 h of release, respectively. CONCLUSION: The optimised ratio between MCS and OSA to prepare in situ cross-linked hydrogels were found to be 5:5 according to the results of DEE and in vitro drug release profiles of MTZ and the MTZ loaded MCS-OSA hydrogels have a great potential which can be applied in biomedical applications. Copyright:
AIM: This study was aimed to prepare in situ cross-linked N-maleoyl chitosan - oxidised sodium alginate (MCS - OSA) hydrogel loaded with metronidazole (MTZ) for drug delivery applications. METHODS: The hydrogel was prepared by in situ cross-linking via Schiff base reaction between amine (-NH2) groups from MCS and aldehyde (-CHO) groups from OSA at the different ratio, and the MTZ was loaded into the hydrogels along with the gelatin processes. RESULTS: The highest drug entrapment efficiency (DEE) was exhibited by MTZ-H3 (5: 5) with DEE of 99.20% and a gel fraction of 97.52%. FTIR results revealed that Schiff base reaction was occurred by the absorption peak of -C = N- groups at 1628 cm-1 and indicated that there is insignificant alteration at different ratio of MCS and OSA. The best sustained of in vitro release profiles of MTZ was shown by MTZ-H3, which is 74.92% and 75.65% at pH 1.2 and 7.4 for 12 h of release, respectively. CONCLUSION: The optimised ratio between MCS and OSA to prepare in situ cross-linked hydrogels were found to be 5:5 according to the results of DEE and in vitro drug release profiles of MTZ and the MTZ loaded MCS-OSA hydrogels have a great potential which can be applied in biomedical applications. Copyright:
Authors: Camila F Rediguieri; Valentina Porta; Diana S G Nunes; Taina M Nunes; Hans E Junginger; Sabine Kopp; Kamal K Midha; Vinod P Shah; Salomon Stavchansky; Jennifer B Dressman; Dirk M Barends Journal: J Pharm Sci Date: 2011-01-19 Impact factor: 3.534