Yun Kyung Bae1, Gee-Hye Kim1, Ji Hye Kwon1, Miyeon Kim1, Soo Jin Choi1, Wonil Oh1, Soyoun Um2, Hye Jin Jin3. 1. Biomedical Research Institute, MEDIPOST Co. Ltd, 21, Daewangpangyo-ro 644-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, 13494, Korea. 2. Biomedical Research Institute, MEDIPOST Co. Ltd, 21, Daewangpangyo-ro 644-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, 13494, Korea. ssoso23@medi-post.co.kr. 3. Biomedical Research Institute, MEDIPOST Co. Ltd, 21, Daewangpangyo-ro 644-gil, Bundang-gu, Seongnam-si, Gyeonggi-do, 13494, Korea. genny77@medi-post.co.kr.
Abstract
BACKGROUND: Regeneration of soft tissue defects is essential for adipose tissue pathologies and disease, trauma, or injury-induced damage. Here, we show that umbilical cord blood-derived mesenchymal stem cells could potentially be tailored and used for the reconstruction of specific damaged sites. Adipogenesis can be exploited in soft tissue reconstruction. Also, primary cilia play a role in the control of adipogenesis. METHODS: The adipogenic differentiation capacity of mesenchymal stem cells (MSCs) was shown to influence ciliogenesis. MSCs transfected with intraflagellar transport 88 (IFT88) small interfering RNA (siRNA), which blocks the assembly and maintenance of cilia, were examined to confirm the relationship between adipogenesis and ciliogenesis. Also, 1,2-Bis(2-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM), calcium chelator, inhibited the ciliogenesis of MSCs in adipogenic differentiation. RESULTS: IFT88-knockdown led to decreased cilia formation and limitation of cilia elongation in adipogenesis. Additionally, intracellular calcium triggered cilia formation in MSCs adipogenesis. Interestingly, intracellular calcium cannot overcome the inhibition of adipogenesis caused by low numbers of cilia in MSCs. CONCLUSION: Our data suggested that ciliogenesis was negatively regulated by Wnt5a/β-catenin signaling during adipogenesis. Thus, we suggest that calcium induction triggers adipogenesis and ciliogenesis.
BACKGROUND: Regeneration of soft tissue defects is essential for adipose tissue pathologies and disease, trauma, or injury-induced damage. Here, we show that umbilical cord blood-derived mesenchymal stem cells could potentially be tailored and used for the reconstruction of specific damaged sites. Adipogenesis can be exploited in soft tissue reconstruction. Also, primary cilia play a role in the control of adipogenesis. METHODS: The adipogenic differentiation capacity of mesenchymal stem cells (MSCs) was shown to influence ciliogenesis. MSCs transfected with intraflagellar transport 88 (IFT88) small interfering RNA (siRNA), which blocks the assembly and maintenance of cilia, were examined to confirm the relationship between adipogenesis and ciliogenesis. Also, 1,2-Bis(2-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid tetrakis(acetoxymethyl ester) (BAPTA-AM), calcium chelator, inhibited the ciliogenesis of MSCs in adipogenic differentiation. RESULTS:IFT88-knockdown led to decreased cilia formation and limitation of cilia elongation in adipogenesis. Additionally, intracellular calcium triggered cilia formation in MSCs adipogenesis. Interestingly, intracellular calcium cannot overcome the inhibition of adipogenesis caused by low numbers of cilia in MSCs. CONCLUSION: Our data suggested that ciliogenesis was negatively regulated by Wnt5a/β-catenin signaling during adipogenesis. Thus, we suggest that calcium induction triggers adipogenesis and ciliogenesis.
Authors: Hye Jin Jin; Ji Hye Kwon; Miyeon Kim; Yun Kyung Bae; Soo Jin Choi; Wonil Oh; Yoon Sun Yang; Hong Bae Jeon Journal: Stem Cells Transl Med Date: 2016-03-03 Impact factor: 6.940
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