V A Khokhlova1,2,3,4,5, P B Rosnitskiy1,2,3,4,5, S A Tsysar1,2,3,4,5, S V Buravkov1,2,3,4,5, O A Sapozhnikov1,2,3,4,5, M M Karzova1,2,3,4,5, T D Khokhlova1,2,3,4,5, A D Maxwell1,2,3,4,5, N M Gaifullin1,2,3,4,5, A V Kadrev1,2,3,4,5, D A Okhobotov1,2,3,4,5, A A Kamalov1,2,3,4,5, G R Schade1,2,3,4,5. 1. Physics Faculty, Lomonosov Moscow State University, Moscow, Russia. 2. Center for Industrial and Medical Ultrasound, University of Washington, Seattle, USA. 3. Faculty of Fundamental Medicine, Lomonosov Moscow State University, Moscow, Russia. 4. Department of Urology, University of Washington, Seattle, USA. 5. Medical Research and Educational Center, Lomonosov Moscow State University, Moscow, Russia.
Abstract
AIM: of the study: demonstrate the feasibility of non-invasive mechanical disintegration of human prostate tissue using pulsed high-intensity focused ultrasound (pHIFU), a method termed boiling histotripsy. MATERIALS AND METHODS: An ultrasound experimental system was developed for producing localized mechanical lesions in ex vivo biological tissue samples under ultrasound guidance. A series of experiments was carried out to create small single-focus lesions (volume < 2 mm3) and one large lesion (volume > 50 mm3) in ex vivo prostate tissue samples. After irradiation, two samples were bisected to visualize the region of destruction; the other tissue samples were examined histologically. RESULTS: During pHIFU irradiation under B-mode ultrasound guidance, a region of increased echogenicity caused by formation of vapor-gas bubbles was visualized in the target region. After exposure, small and large lesions filled with a suspension of liquefied tissue were observed. Histological examination confirmed that the prostate tissue in the focal region was disintegrated into subcellular fragments. CONCLUSION: A pilot study showed the feasibility of using boiling histotripsy as a non-invasive method for treating prostate diseases.
AIM: of the study: demonstrate the feasibility of non-invasive mechanical disintegration of human prostate tissue using pulsed high-intensity focused ultrasound (pHIFU), a method termed boiling histotripsy. MATERIALS AND METHODS: An ultrasound experimental system was developed for producing localized mechanical lesions in ex vivo biological tissue samples under ultrasound guidance. A series of experiments was carried out to create small single-focus lesions (volume < 2 mm3) and one large lesion (volume > 50 mm3) in ex vivo prostate tissue samples. After irradiation, two samples were bisected to visualize the region of destruction; the other tissue samples were examined histologically. RESULTS: During pHIFU irradiation under B-mode ultrasound guidance, a region of increased echogenicity caused by formation of vapor-gas bubbles was visualized in the target region. After exposure, small and large lesions filled with a suspension of liquefied tissue were observed. Histological examination confirmed that the prostate tissue in the focal region was disintegrated into subcellular fragments. CONCLUSION: A pilot study showed the feasibility of using boiling histotripsy as a non-invasive method for treating prostate diseases.
Authors: Christopher R Bawiec; Tatiana D Khokhlova; Oleg A Sapozhnikov; Pavel B Rosnitskiy; Bryan W Cunitz; Mohamed A Ghanem; Christopher Hunter; Wayne Kreider; George R Schade; Petr V Yuldashev; Vera A Khokhlova Journal: IEEE Trans Ultrason Ferroelectr Freq Control Date: 2021-04-26 Impact factor: 2.725