Eduardo Vargas-Baquero1, Stephen Johnston2, Antonio Sánchez-Ramos3, Angel Arévalo-Martín1, Richard Wilson4, Jaime Gosálvez5. 1. Unidad de Sexualidad y Fertilidad, Hospital Nacional de Parapléjicos de Toledo, Toledo, Spain. 2. School of Agriculture and Food Sciences, University of Queensland, Gatton, QLD, Australia. s.johnston1@uq.edu.au. 3. Unidad de Sexualidad de la Fundación Lesionado Medular de Madrid, Madrid, Spain. 4. School of Mathematics and Physics, University of Queensland, St Lucia, QLD, Australia. 5. Unidad de Genética, Departamento de Biología, Universidad Autónoma de Madrid, Madrid, Spain.
Abstract
STUDY DESIGN: Retrospective descriptive study. OBJECTIVES: To determine the incidence and probable etiology of sperm DNA fragmentation (SDF) in a sample of males with spinal cord injury (SCI). SETTING: Hospital in Toledo, Spain; University-based Genetics laboratory in Madrid, Spain. METHODS: Semen collected by vibro-stimulation from 27 males with various levels of spinal cord injury. Classical semen parameters, SDF, leukocytospermia and pro-oxidant capacity were assessed and compared with a cohort of normozoospermic fertile donors (n = 10). RESULTS: Males with SCI presented with lower semen quality compared with normozoospermic donors with respect to progressive motility (p = 0.0002), SDF (p < 0.00005), pro-oxidant capacity (p = 0.0191) and leukocytospermia (p < 0.00005). Although there was no significant correlation between semen quality and time since the lesion occurred, the period of abstinence appeared to be positively correlated with SDF (r = 0.486; p = 0.041). When the semen parameters of males with SCI were categorized based on those with cervical and thoracic lesions, sperm concentration was higher for those with cervical damage (p = 0.0257). Males with complete lesions (AIS A) had ejaculates that were lower in progressive motility (p = 0.0040) than those with incomplete injuries (AIS B-D). CONCLUSIONS: Ejaculates of males with SCI have excessively elevated SDF when compared with normozoospermic donors, which is likely to be associated with coincident high levels of leucocytospermia and pro-oxidant capacity. We propose that these phenomena are caused by the accumulation and degeneration of spermatozoa in the cauda epididymidis.
STUDY DESIGN: Retrospective descriptive study. OBJECTIVES: To determine the incidence and probable etiology of sperm DNA fragmentation (SDF) in a sample of males with spinal cord injury (SCI). SETTING: Hospital in Toledo, Spain; University-based Genetics laboratory in Madrid, Spain. METHODS: Semen collected by vibro-stimulation from 27 males with various levels of spinal cord injury. Classical semen parameters, SDF, leukocytospermia and pro-oxidant capacity were assessed and compared with a cohort of normozoospermic fertile donors (n = 10). RESULTS: Males with SCI presented with lower semen quality compared with normozoospermic donors with respect to progressive motility (p = 0.0002), SDF (p < 0.00005), pro-oxidant capacity (p = 0.0191) and leukocytospermia (p < 0.00005). Although there was no significant correlation between semen quality and time since the lesion occurred, the period of abstinence appeared to be positively correlated with SDF (r = 0.486; p = 0.041). When the semen parameters of males with SCI were categorized based on those with cervical and thoracic lesions, sperm concentration was higher for those with cervical damage (p = 0.0257). Males with complete lesions (AIS A) had ejaculates that were lower in progressive motility (p = 0.0040) than those with incomplete injuries (AIS B-D). CONCLUSIONS: Ejaculates of males with SCI have excessively elevated SDF when compared with normozoospermic donors, which is likely to be associated with coincident high levels of leucocytospermia and pro-oxidant capacity. We propose that these phenomena are caused by the accumulation and degeneration of spermatozoa in the cauda epididymidis.
Authors: Sandro C Esteves; Jaime Gosálvez; Carmen López-Fernández; Rocío Núñez-Calonge; Pedro Caballero; Ashok Agarwal; José Luis Fernández Journal: Int Urol Nephrol Date: 2015-07-31 Impact factor: 2.370
Authors: Rakesh Sharma; Sajal Gupta; Ashok Agarwal; Ralf Henkel; Renata Finelli; Neel Parekh; Ramadan Saleh; Mohamed Arafa; Edmund Ko; Armand Zini; Nicholas Tadros; Rupin Shah; Rafael F Ambar; Haitham Elbardisi; Pallav Sengupta; Marlon Martinez; Florence Boitrelle; Mara Simopoulou; Paraskevi Vogiatzi; Jaime Gosalvez; Parviz Kavoussi; Hussein Kandil; Ayad Palani; Marcelo Rodriguez Peña; Osvaldo Rajmil; Gian Maria Busetto; Christina Anagnostopoulou; Sava Micic; Marco G Alves; Lucia Rocco; Taymour Mostafa; Juan G Alvarez; Sunil Jindal; Hassan N Sallam; Israel Maldonado Rosas; Sheena E M Lewis; Sami AlSaid; Mesut Altan; Hyun Jun Park; Jonathan Ramsay; Sijo Parekattil; Marjan Sabbaghian; Kelton Tremellen; Kareim Khalafalla; Damayanthi Durairajanayagam; Giovanni M Colpi Journal: World J Mens Health Date: 2021-06-09 Impact factor: 5.400