Literature DB >> 31999448

Semisynthesis of Plant-Derived Englerin A Enabled by Microbe Engineering of Guaia-6,10(14)-diene as Building Block.

Thomas Siemon1, Zhangqian Wang2, Guangkai Bian2, Tobias Seitz1, Ziling Ye3, Yan Lu2, Shu Cheng2, Yunkun Ding2, Yanglei Huang2, Zixin Deng2,4, Tiangang Liu2,4, Mathias Christmann1.   

Abstract

Herein, we report a short semisynthesis of the potent transient receptor potential canonical (TRPC) channel agonist englerin A (EA) and the related guaianes oxyphyllol and orientalol E. The guaia-6,10(14)-diene starting material was systematically engineered in Escherichia coli and Saccharomyces cerevisiae using the CRISPR/Cas9 system and was produced with high titers. The potentially scalable approach combines the advantages of synthetic biology and chemical synthesis providing an efficient and economical method for producing EA and analogues.

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Year:  2020        PMID： 31999448     DOI： 10.1021/jacs.9b12940

Source DB:  PubMed          Journal:  J Am Chem Soc        ISSN： 0002-7863            Impact factor:   15.419


  6 in total

1.  Revolution of vitamin E production by starting from microbial fermented farnesene to isophytol.

Authors:  Ziling Ye; Bin Shi; Yanglei Huang; Tian Ma; Zilei Xiang; Ben Hu; Zhaolin Kuang; Man Huang; Xiaoying Lin; Zhu Tian; Zixin Deng; Kun Shen; Tiangang Liu
Journal:  Innovation (Camb)       Date:  2022-03-12

2.  Identification of the sesquiterpene synthase AcTPS1 and high production of (-)-germacrene D in metabolically engineered Saccharomyces cerevisiae.

Authors:  Jiajia Liu; Chang Chen; Xiukun Wan; Ge Yao; Shaoheng Bao; Fuli Wang; Kang Wang; Tianyu Song; Penggang Han; Hui Jiang
Journal:  Microb Cell Fact       Date:  2022-05-18       Impact factor: 6.352

Review 3.  Complex natural product production methods and options.

Authors:  Dongwon Park; Girish Swayambhu; Thomas Lyga; Blaine A Pfeifer
Journal:  Synth Syst Biotechnol       Date:  2021-01-05

Review 4.  Predictive Engineering of Class I Terpene Synthases Using Experimental and Computational Approaches.

Authors:  Nicole G H Leferink; Nigel S Scrutton
Journal:  Chembiochem       Date:  2021-11-03       Impact factor: 3.461

5.  The Biosynthetic Gene Cluster for Sestermobaraenes-Discovery of a Geranylfarnesyl Diphosphate Synthase and a Multiproduct Sesterterpene Synthase from Streptomyces mobaraensis.

Authors:  Anwei Hou; Jeroen S Dickschat
Journal:  Angew Chem Int Ed Engl       Date:  2020-08-31       Impact factor: 15.336

Review 6.  Alternative metabolic pathways and strategies to high-titre terpenoid production in Escherichia coli.

Authors:  Mauro A Rinaldi; Clara A Ferraz; Nigel S Scrutton
Journal:  Nat Prod Rep       Date:  2022-01-26       Impact factor: 13.423
  6 in total

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