| Literature DB >> 3199842 |
B G Campling1, J Pym, P R Galbraith, S P Cole.
Abstract
A microcytotoxicity assay employing a tetrazolium salt has been adapted for testing the response of human leukemic blast cells to a variety of chemotherapeutic agents. After exposure to various concentrations of drugs, the viability of fresh leukemic blast cells was measured using a tetrazolium salt, MTT, which is converted to blue formazan crystals by living cells. The amount of formazan produced was quantitated using a microtitre plate spectrophotometer. In the present study, optimal conditions for chemosensitivity testing of human leukemia samples were determined, and the relative chemosensitivity of five patient samples was tested.Entities:
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Year: 1988 PMID： 3199842 DOI： 10.1016/0145-2126(88)90036-7
Source DB: PubMed Journal: Leuk Res ISSN： 0145-2126 Impact factor: 3.156