Xiuli He1, Franz-Xaver Reichl1, Stefan Milz2, Bernhard Michalke3, Xiao Wu4, Christoph M Sprecher5, Yang Yang6, Michael Gahlert7, Stefan Röhling8, Heinz Kniha9, Reinhard Hickel10, Christof Högg11. 1. Department of Conservative Dentistry and Periodontology, University Hospital, LMU Munich, Germany; Walther Straub Institute of Pharmacology and Toxicology, Faculty of Medicine, LMU Munich, Germany. 2. Institute of Anatomy, Neuroanatomy, Faculty of Medicine, LMU Munich, Germany. 3. Research Unit Analytical Biogeochemistry, Helmholtz Zentrum München, German Research Center for Environmental Health, Germany. 4. Helmholtz Zentrum München, German Research Center for Environmental Health Cooperation Group of Comprehensive Molecular Analytics, Germany. 5. AO Research Institute, AO Foundation, Davos Platz, Switzerland. 6. Department of Conservative Dentistry and Periodontology, University Hospital, LMU Munich, Germany; Key Laboratory of Oral Medicine, Guangzhou Institute of Oral Disease, Stomatology Hospital of Guangzhou Medical University, Guangzhou 510140, China. 7. Private Dental Clinic Drs. Gahlert and Röhling, Munich, Germany; Sigmund Freud University Vienna, Department of Oral Surgery, Austria; Clinic for Oral and Cranio-Maxillofacial Surgery, Hightech Research Center, University Hospital Basel, Basel, Switzerland. 8. Private Dental Clinic Drs. Gahlert and Röhling, Munich, Germany; Clinic for Oral and Cranio-Maxillofacial Surgery, Kantonsspital Aarau, Aarau, Switzerland. 9. Private Dental Clinic Drs. Kniha and Schlegel, Munich, Germany. 10. Department of Conservative Dentistry and Periodontology, University Hospital, LMU Munich, Germany. 11. Department of Conservative Dentistry and Periodontology, University Hospital, LMU Munich, Germany; Walther Straub Institute of Pharmacology and Toxicology, Faculty of Medicine, LMU Munich, Germany. Electronic address: christof.hoegg@lrz.uni-muenchen.de.
Abstract
OBJECTIVE: Titanium (Ti)- and Zirconia (ZrO2)-implants in mini pig maxillae were compared with respect to Ti/zirconium (Zr) release into the surrounding bone tissues, the resulting short term tissue responses and the potential toxicity. METHODS: Ti/Zr release from Ti- and ZrO2-implants in mini pig maxillae was determined with inductively coupled plasma optical emission spectrometry (ICP-OES) and inductively coupled plasma mass spectrometry (ICP-MS). The spatial distribution of Ti and Zr in maxilla tissues near the implant surface was assessed with laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). A histological analysis was performed to investigate the tissue responses after 12 weeks of implantation. The cytotoxicity and DNA damage of Ti particles and ZrO2 particles were studied with XTT and Comet assay. RESULTS: The mean Ti content in the bone adjacent to Ti-implants was 1.67 mg/kg-bone weight. The highest Ti content detected was 2.17 mg/kg-bone weight. The mean Zr content in the bone adjected to ZrO2-implants was 0.59 mg/kg-bone weight. The highest Zr content was 0.75 mg/kg-bone weight. The spatial distribution of the Ti and Zr in bone showed mainly a higher intensity of Ti and Zr close to the screw thread outer tip rather. Histological analysis indicated that near both implant-types signs of bone marrow fibrosis were present. EC50 of commercially available ZrO2-nanoparticles (NPs, <100 nm) and ZrO2-microparticles (MPs, <5 μm) was 13.96 mg/ml and 80.99 mg/ml, respectively. ZrO2-NPs and ZrO2-MPs can induce DNA damage at 70 μg/ml and 810 μg/ml, respectively. SIGNIFICANCE: After 12-weeks of implantation, increased concentrations of Ti and Zr can be detected in bone/tissues near Ti- and ZrO2-implants in mini pig maxillae. Ti content released from Ti-implants is two times higher than the Zr content released from ZrO2-implants. ZrO2-NPs showed lower cytotoxicity and DNA damage compared to results reported for Ti-NPs in human cells.
OBJECTIVE:Titanium (Ti)- and Zirconia (ZrO2)-implants in mini pig maxillae were compared with respect to Ti/zirconium (Zr) release into the surrounding bone tissues, the resulting short term tissue responses and the potential toxicity. METHODS:Ti/Zr release from Ti- and ZrO2-implants in mini pig maxillae was determined with inductively coupled plasma optical emission spectrometry (ICP-OES) and inductively coupled plasma mass spectrometry (ICP-MS). The spatial distribution of Ti and Zr in maxilla tissues near the implant surface was assessed with laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS). A histological analysis was performed to investigate the tissue responses after 12 weeks of implantation. The cytotoxicity and DNA damage of Ti particles and ZrO2 particles were studied with XTT and Comet assay. RESULTS: The mean Ti content in the bone adjacent to Ti-implants was 1.67 mg/kg-bone weight. The highest Ti content detected was 2.17 mg/kg-bone weight. The mean Zr content in the bone adjected to ZrO2-implants was 0.59 mg/kg-bone weight. The highest Zr content was 0.75 mg/kg-bone weight. The spatial distribution of the Ti and Zr in bone showed mainly a higher intensity of Ti and Zr close to the screw thread outer tip rather. Histological analysis indicated that near both implant-types signs of bone marrow fibrosis were present. EC50 of commercially available ZrO2-nanoparticles (NPs, <100 nm) and ZrO2-microparticles (MPs, <5 μm) was 13.96 mg/ml and 80.99 mg/ml, respectively. ZrO2-NPs and ZrO2-MPs can induce DNA damage at 70 μg/ml and 810 μg/ml, respectively. SIGNIFICANCE: After 12-weeks of implantation, increased concentrations of Ti and Zr can be detected in bone/tissues near Ti- and ZrO2-implants in mini pig maxillae. Ti content released from Ti-implants is two times higher than the Zr content released from ZrO2-implants. ZrO2-NPs showed lower cytotoxicity and DNA damage compared to results reported for Ti-NPs in human cells.
Authors: Ali Al-Ahmad; Ali Modabber; Kristian Kniha; Eva Miriam Buhl; Stephan Christian Möhlhenrich; Anna Bock; Frank Hölzle; Elmar Hellwig Journal: BMC Oral Health Date: 2021-12-31 Impact factor: 2.757
Authors: Kristian Kniha; Lothar Rink; Jana Wolf; Stephan Christian Möhlhenrich; Florian Peters; Marius Heitzer; Frank Hölzle; Ali Modabber Journal: Sci Rep Date: 2022-03-15 Impact factor: 4.379
Authors: Michael Nemec; Christian Behm; Vera Maierhofer; Jonas Gau; Anastasiya Kolba; Erwin Jonke; Xiaohui Rausch-Fan; Oleh Andrukhov Journal: Int J Mol Sci Date: 2022-09-02 Impact factor: 6.208