| Literature DB >> 31988827 |
Chandra Mohan Singh1,2, Aditya Pratap1, Sanjeev Gupta1, Revanappa S Biradar3, Narendra Pratap Singh1.
Abstract
The present study aimed to detect the marker-trait association of a selected diverse panel of 127 mungbean genotypes against mungbean yellow mosaic India virus (MYMIV). Virus-specific primers pairs viz., AC-abut/AV-abut and BC-abut/BV-abut confirmed the involvement of MYMIV in yellow mosaic disease development and the same was validated through restriction digestion analysis. 256 genome-wide microsatellite markers were screened on a test panel in which 93 polymorphic markers were used in association studies. Population structure analysis led to formation of six distinct subpopulations. 1097 alleles were detected among 127 test genotypes whereas number of alleles ranged 2-22 and PIC values ranged 0.27-0.92%, indicating ample amount of variation at genome level. 15 microsatellite markers were detected as associated with MYMIV resistance, among them three microsatellites explained 11-14% phenotypic variation. The specific regions close to CEDG293, DMB-SSR008 and DMB-SSR059 associated with MYMIV resistance were detected, located on linkage group 2, 4 and 9 and may prove useful in marker-assisted mungbean improvement programme for enhancing MYMIV resistance. © King Abdulaziz City for Science and Technology 2020.Entities:
Keywords: MYMIV; Marker-trait association; Simple sequence repeats; Vigna radiata; YMD
Year: 2020 PMID： 31988827 PMCID： PMC6946765 DOI： 10.1007/s13205-019-2035-7
Source DB: PubMed Journal: 3 Biotech ISSN： 2190-5738 Impact factor: 2.406