Seyed-Amir Tabatabaeizadeh1, Mostafa Fazeli2, Zahra Meshkat3, Ezzat Khodashenas4, Habibollah Esmaeili5, Seyedreza Mazloum6, Gordon A Ferns7, Mojtaba Fattahi Abdizadeh8, Majid Ghayour-Mobarhan9. 1. Nutrition and Biochemistry Department, School of Medicine, Social Development and Health Promotion Research Center, Gonabad University of Medical Sciences, Gonabad, Iran. 2. Genetic and Molecular Medicine Department, School of Medicine, Mashhad University of Medical Sciences, Iran. 3. Antimicrobial Resistance Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. 4. Department of Pediatrics, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. 5. Departments of Biostatistics and Epidemiology, School of Health, Mashhad University of Medical Sciences, Mashhad, Iran. 6. Evidence Based Care Research Center, Faculty Member of Nursing and Midwifery School, Mashhad University of Medical Sciences, Mashhad, Iran. 7. Brighton & Sussex Medical School, Division of Medical Education, Falmer, Brighton, Sussex, BN1 9PH, UK. 8. Department of Microbiology, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran. 9. Metabolic Syndrome Research Center, School of Medicine, Mashhad University of Medical Science, Mashhad, Iran. Electronic address: ghayourm@mums.ac.ir.
Abstract
BACKGROUND: Animal studies suggest that vitamin D can change the gut microbiome. The primary aim of this study was to evaluate the effect of a high dose supplementation of vitamin D on the composition of the gut microbiome. METHODS: After DNA extraction, TaqMan assays were used for the quantitation of selected microbiome in the feces of 50 adolescent girls before and after vitamin D supplementation. RESULTS: The expression fold changes for Enterococcus, Bifidobacterium, Lactobacillus, Bacteroidetes and Firmicutes were; 1.05, 1.20, 0.76, 0.28 and 1.50 respectively. Bacteroidetes and Lactobacillus fell by 72% (P < 0.0001) and 24% (P = 0.006) respectively, whilst Firmicutes and Bifidobacterium were increased by 1.5 (P < 0.0001), 1.2 (P < 0.0001) fold after supplementation. CONCLUSION: Our results suggested that a high dose supplementation of vitamin D alter the human gut microbiome composition. Future studies are required for a better understanding of the mechanisms by which vitamin D affects the gut microbiome.
BACKGROUND: Animal studies suggest that vitamin D can change the gut microbiome. The primary aim of this study was to evaluate the effect of a high dose supplementation of vitamin D on the composition of the gut microbiome. METHODS: After DNA extraction, TaqMan assays were used for the quantitation of selected microbiome in the feces of 50 adolescent girls before and after vitamin D supplementation. RESULTS: The expression fold changes for Enterococcus, Bifidobacterium, Lactobacillus, Bacteroidetes and Firmicutes were; 1.05, 1.20, 0.76, 0.28 and 1.50 respectively. Bacteroidetes and Lactobacillus fell by 72% (P < 0.0001) and 24% (P = 0.006) respectively, whilst Firmicutes and Bifidobacterium were increased by 1.5 (P < 0.0001), 1.2 (P < 0.0001) fold after supplementation. CONCLUSION: Our results suggested that a high dose supplementation of vitamin D alter the humangut microbiome composition. Future studies are required for a better understanding of the mechanisms by which vitamin D affects the gut microbiome.
Authors: Huicui Meng; Nirupa R Matthan; Edith Angellotti; Anastassios G Pittas; Alice H Lichtenstein Journal: Am J Clin Nutr Date: 2020-09-01 Impact factor: 7.045