Literature DB >> 31986363

Characterizing membrane phospholipid hydrolysis of pork loins throughout three aging periods.

M D Chao1, E A Donaldson2, W Wu2, A A Welter2, T G O'Quinn2, W-W Hsu3, M D Schulte4, S M Lonergan4.   

Abstract

Three chops from 20 pork carcasses were aged for 1, 8, and 21 days. Electrospray ionization-tandem mass spectrometry was used to comprehensively analyze profiles of phospholipids from each sample (n = 60). Total phospholipid quantity decreased 4-folds (P < .01) from 1 to 21 days of aging in pork loins. Phosphatidylinositol (PI) and phosphatidylserine (PS) increased by 30% and 73%, respectively, from 1 to 21 days of aging in pork loins (P < .01). This increase was mainly due to relative percentage increase from PI 38:4 (18:0-20:4) and PS 36:2 (18:0-18:2; P < .01). The results also showed that the relative percentage of lysophosphatidylcholine increased by 35% after short term aging (8d), and phosphatidic acid increased by 10-folds after extended aging (21d; P < .01). These results documented that phospholipids undergo enzymatic hydrolysis during aging, but also indicated that lipid species containing 18:2 or 20:4 within PI and PS were slightly more resistant to enzymatic hydrolysis compared with the other phospholipids.
Copyright © 2020 Elsevier Ltd. All rights reserved.

Entities:  

Keywords:  Aging; Hydrolysis; Lipidomics; Phospholipid; Pork

Mesh:

Substances:

Year:  2020        PMID: 31986363      PMCID: PMC7958495          DOI: 10.1016/j.meatsci.2020.108065

Source DB:  PubMed          Journal:  Meat Sci        ISSN: 0309-1740            Impact factor:   5.209


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