M Raad1, Z Salehi1, M Habibzaadeh Baalsini1, F Mashayekhi1, H Saeidi Saedi2. 1. Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran. 2. Department of Radiation Oncology, Cancer Research Center, Guilan University of Medical Sciences (GUMS), Rasht, Iran.
Abstract
Background: Gastric cancer is a complicated malignancy whose aetiology is not well characterized. Single nucleotide polymorphisms (SNPs), some located within microRNA genes, are linked with gastric cancer. We hypothesized a link between SNP rs2620381 (A > C) in miR-627 and gastric cancer.Material and methods: We recruited 280 healthy controls and 240 gastric cancer patients. Genotyping was conducted by allele-specific polymerase chain reaction. In addition, in silico analyses were carried out via databases and web tools including miRBase, dbSNP, RNAfold, MiRNASNP V2.0, miRWalk V2.0, miRTarBase, and miRmap. Results: Any C genotype in rs2620381 was linked to gastric cancer: CC vs. AA: OR/95% CI 2.67 (1.17-6.09), p = 0.01, CC+AC vs. AA: OR/95% CI 1.66 (1.12-2.46), p = 0.01, CC vs. AC+AA: OR/95% CI 2.44 (1.07-5.54), p = 0.03. The minor allele C of miR-627 was linked with gastric cancer compared with A allele (OR/95%CI 1.88 (1.30-2.73), p = 0.0008). There were no links between age, sex, tumour type, distant metastasis, and tumour stages and the miR-627 polymorphism in gastric cancer patients. Conclusion: Presence of the C SNP in miR-627 rs2620381 in linked with gastric cancer, and may be important in pathogenesis.
Background: Gastric cancer is a complicated malignancy whose aetiology is not well characterized. Single nucleotide polymorphisms (SNPs), some located within microRNA genes, are linked with gastric cancer. We hypothesized a link between SNP rs2620381 (A > C) in miR-627 and gastric cancer.Material and methods: We recruited 280 healthy controls and 240 gastric cancerpatients. Genotyping was conducted by allele-specific polymerase chain reaction. In addition, in silico analyses were carried out via databases and web tools including miRBase, dbSNP, RNAfold, MiRNASNP V2.0, miRWalk V2.0, miRTarBase, and miRmap. Results: Any C genotype in rs2620381 was linked to gastric cancer: CC vs. AA: OR/95% CI 2.67 (1.17-6.09), p = 0.01, CC+AC vs. AA: OR/95% CI 1.66 (1.12-2.46), p = 0.01, CC vs. AC+AA: OR/95% CI 2.44 (1.07-5.54), p = 0.03. The minor allele C of miR-627 was linked with gastric cancer compared with A allele (OR/95%CI 1.88 (1.30-2.73), p = 0.0008). There were no links between age, sex, tumour type, distant metastasis, and tumour stages and the miR-627 polymorphism in gastric cancerpatients. Conclusion: Presence of the C SNP in miR-627rs2620381 in linked with gastric cancer, and may be important in pathogenesis.
Entities:
Keywords:
Gastric cancer; SNP; in silico analysis; miR-627; microRNA; polymorphism