Minhao Hu1, Lejun Li1, Shuyuan Liu2, Yiyun Lou3, Liya Wang1, Fang Le1, Hongping Li1, Qijing Wang1, Hangying Lou1, Ning Wang1, Fan Jin4,5. 1. Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, 1 Xueshi Road, Hangzhou, 310006, Zhejiang, China. 2. Department of Gynecology, Weifang Maternal and Child Health Hospital, Weifang, 261000, China. 3. Department of Gynecology, Hangzhou Hospital of Traditional Chinese Medicine, Hangzhou, 310007, China. 4. Department of Reproductive Endocrinology, Women's Hospital, Zhejiang University School of Medicine, 1 Xueshi Road, Hangzhou, 310006, Zhejiang, China. jinfan@zju.edu.cn. 5. Women's Reproductive Health Laboratory of Zhejiang Province, Key Laboratory of Reproductive Genetics, Ministry of Education, Hangzhou, 310006, China. jinfan@zju.edu.cn.
Abstract
PURPOSE: To assess testicular mRNA and protein expression levels of MRE11 and RAD50 in human azoospermia patients. METHODS: Patients diagnosed with maturation arrest at the spermatocyte stage (MA) and Sertoli cell-only syndrome (SCOS) were recruited through diagnostic testicular biopsy. Patients with normal spermatogenesis were studied as controls. In addition, knockdown of MRE11 and RAD50 was performed in GC-2spd(ts) cells to investigate their roles in cellular proliferation and apoptosis. RESULTS: mRNA and protein expression levels of MRE11 and RAD50 were measured using quantitative polymerase chain reaction, western blotting, and immunohistochemistry, respectively. Knockdown of both MRE11 and RAD50 utilized transfection with small interfering RNAs. CONCLUSION: Our findings demonstrated altered expression levels of MRE11 and RAD50 in human testes with MA and SCOS, and showed that these alterations might be associated with impaired spermatogenesis. These results offer valuable new perspectives into the molecular mechanisms of male infertility.
PURPOSE: To assess testicular mRNA and protein expression levels of MRE11 and RAD50 in humanazoospermiapatients. METHODS:Patients diagnosed with maturation arrest at the spermatocyte stage (MA) and Sertoli cell-only syndrome (SCOS) were recruited through diagnostic testicular biopsy. Patients with normal spermatogenesis were studied as controls. In addition, knockdown of MRE11 and RAD50 was performed in GC-2spd(ts) cells to investigate their roles in cellular proliferation and apoptosis. RESULTS: mRNA and protein expression levels of MRE11 and RAD50 were measured using quantitative polymerase chain reaction, western blotting, and immunohistochemistry, respectively. Knockdown of both MRE11 and RAD50 utilized transfection with small interfering RNAs. CONCLUSION: Our findings demonstrated altered expression levels of MRE11 and RAD50 in human testes with MA and SCOS, and showed that these alterations might be associated with impaired spermatogenesis. These results offer valuable new perspectives into the molecular mechanisms of male infertility.
Authors: Julian Lange; Shintaro Yamada; Sam E Tischfield; Jing Pan; Seoyoung Kim; Xuan Zhu; Nicholas D Socci; Maria Jasin; Scott Keeney Journal: Cell Date: 2016-10-13 Impact factor: 41.582