Literature DB >> 31981039

A One-Step Process for the Construction of Phage Display scFv and VHH Libraries.

Carolin Sellmann1, Lukas Pekar1, Christina Bauer1, Elke Ciesielski1, Simon Krah1, Stefan Becker1, Lars Toleikis1, Jonas Kügler2, André Frenzel2, Bernhard Valldorf3, Michael Hust2,4, Stefan Zielonka5.   

Abstract

In this work we present a one-step cloning approach for the establishment of antibody phage display libraries relying on type IIs restriction enzymes. We show that single chain variable fragment (scFv) libraries with adequate qualities can readily be cloned in a 'scar-less' manner and that the isolation of antigen-specific antibodies from immunized chickens is feasible within three selection rounds. Moreover, we demonstrate the general applicability of this method by rapidly constructing and panning VHH single domain antibody phage display libraries from immunized Llama repertoires.

Entities:  

Keywords:  Antibody; Golden gate cloning; Phage display; Single domain antibody; Type IIs enzyme; VHH; scFv

Mesh：

Substances：

Year:  2020        PMID： 31981039     DOI： 10.1007/s12033-020-00236-0

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  37 in total

1.  A large non-immunized human Fab fragment phage library that permits rapid isolation and kinetic analysis of high affinity antibodies.

Authors:  H J de Haard; N van Neer; A Reurs; S E Hufton; R C Roovers; P Henderikx; A P de Bruïne; J W Arends; H R Hoogenboom
Journal:  J Biol Chem       Date:  1999-06-25       Impact factor: 5.157

2.  Fully synthetic human combinatorial antibody libraries (HuCAL) based on modular consensus frameworks and CDRs randomized with trinucleotides.

Authors:  A Knappik; L Ge; A Honegger; P Pack; M Fischer; G Wellnhofer; A Hoess; J Wölle; A Plückthun; B Virnekäs
Journal:  J Mol Biol       Date:  2000-02-11       Impact factor: 5.469

Review 3.  Antibody repertoire development in camelids.

Authors:  Erwin De Genst; Dirk Saerens; Serge Muyldermans; Katja Conrath
Journal:  Dev Comp Immunol       Date:  2006       Impact factor: 3.636

4.  A surface expression vector for antibody screening.

Authors:  F Breitling; S Dübel; T Seehaus; I Klewinghaus; M Little
Journal:  Gene       Date:  1991-08-15       Impact factor: 3.688

5.  Facile generation of antibody heavy and light chain diversities for yeast surface display by Golden Gate Cloning.

Authors:  Lukas Roth; Julius Grzeschik; Steffen C Hinz; Stefan Becker; Lars Toleikis; Michael Busch; Harald Kolmar; Simon Krah; Stefan Zielonka
Journal:  Biol Chem       Date:  2019-02-25       Impact factor: 3.915

6.  A human scFv antibody generation pipeline for proteome research.

Authors:  Michael Hust; Torsten Meyer; Bernd Voedisch; Torsten Rülker; Holger Thie; Aymen El-Ghezal; Martina Inga Kirsch; Mark Schütte; Saskia Helmsing; Doris Meier; Thomas Schirrmann; Stefan Dübel
Journal:  J Biotechnol       Date:  2010-09-29       Impact factor: 3.307

7.  Yeast Surface Display in Combination with Fluorescence-activated Cell Sorting Enables the Rapid Isolation of Antibody Fragments Derived from Immunized Chickens.

Authors:  Julius Grzeschik; Desislava Yanakieva; Lukas Roth; Simon Krah; Steffen C Hinz; Adrian Elter; Tina Zollmann; Gerhard Schwall; Stefan Zielonka; Harald Kolmar
Journal:  Biotechnol J       Date:  2018-11-28       Impact factor: 4.677

8.  Generation and analysis of the improved human HAL9/10 antibody phage display libraries.

Authors:  Jonas Kügler; Sonja Wilke; Doris Meier; Florian Tomszak; André Frenzel; Thomas Schirrmann; Stefan Dübel; Henk Garritsen; Björn Hock; Lars Toleikis; Mark Schütte; Michael Hust
Journal:  BMC Biotechnol       Date:  2015-02-19       Impact factor: 2.563

9.  Isolation of high affinity human antibodies directly from large synthetic repertoires.

Authors:  A D Griffiths; S C Williams; O Hartley; I M Tomlinson; P Waterhouse; W L Crosby; R E Kontermann; P T Jones; N M Low; T J Allison
Journal:  EMBO J       Date:  1994-07-15       Impact factor: 11.598

Review 10.  Phage Display Libraries for Antibody Therapeutic Discovery and Development.

Authors:  Juan C Almagro; Martha Pedraza-Escalona; Hugo Iván Arrieta; Sonia Mayra Pérez-Tapia
Journal:  Antibodies (Basel)       Date:  2019-08-23
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  4 in total

1.  Biophysical and biochemical characterization of a VHH-based IgG-like bi- and trispecific antibody platform.

Authors:  Lukas Pekar; Michael Busch; Bernhard Valldorf; Steffen C Hinz; Lars Toleikis; Simon Krah; Stefan Zielonka
Journal:  MAbs       Date:  2020 Jan-Dec       Impact factor: 5.857

2.  Milking the Cow: Cattle-Derived Chimeric Ultralong CDR-H3 Antibodies and Their Engineered CDR-H3-Only Knobbody Counterparts Targeting Epidermal Growth Factor Receptor Elicit Potent NK Cell-Mediated Cytotoxicity.

Authors:  Lukas Pekar; Daniel Klewinghaus; Paul Arras; Stefania C Carrara; Julia Harwardt; Simon Krah; Desislava Yanakieva; Lars Toleikis; Vaughn V Smider; Harald Kolmar; Stefan Zielonka
Journal:  Front Immunol       Date:  2021-10-25       Impact factor: 7.561

Review 3.  High-Throughput Monoclonal Antibody Discovery from Phage Libraries: Challenging the Current Preclinical Pipeline to Keep the Pace with the Increasing mAb Demand.

Authors:  Nicola Zambrano; Guendalina Froechlich; Dejan Lazarevic; Margherita Passariello; Alfredo Nicosia; Claudia De Lorenzo; Marco J Morelli; Emanuele Sasso
Journal:  Cancers (Basel)       Date:  2022-03-04       Impact factor: 6.639

Review 4.  A guide to: generation and design of nanobodies.

Authors:  Serge Muyldermans
Journal:  FEBS J       Date:  2020-08-28       Impact factor: 5.542
  4 in total

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