| Literature DB >> 31980605 |
Ning Li1,2, Anuradha Kalyanasundaram1,2, Brian J Hansen1,2, Esthela J Artiga1,2, Roshan Sharma3, Suhaib H Abudulwahed1,2, Katelynn M Helfrich1,2, Galina Rozenberg1,2, Pei-Jung Wu1,2, Stanislav Zakharkin1, Sandor Gyorke1,2, Paul Ml Janssen1,2, Bryan A Whitson2,4, Nahush A Mokadam2,4, Brandon J Biesiadecki1,2, Federica Accornero1,2, John D Hummel2,5, Peter J Mohler1,2, Halina Dobrzynski6,7, Jichao Zhao3, Vadim V Fedorov8,9.
Abstract
Mechanisms for human sinoatrial node (SAN) dysfunction are poorly understood and whether human SAN excitability requires voltage-gated sodium channels (Nav) remains controversial. Here, we report that neuronal (n)Nav blockade and selective nNav1.6 blockade during high-resolution optical mapping in explanted human hearts depress intranodal SAN conduction, which worsens during autonomic stimulation and overdrive suppression to conduction failure. Partial cardiac (c)Nav blockade further impairs automaticity and intranodal conduction, leading to beat-to-beat variability and reentry. Multiple nNav transcripts are higher in SAN vs atria; heterogeneous alterations of several isoforms, specifically nNav1.6, are associated with heart failure and chronic alcohol consumption. In silico simulations of Nav distributions suggest that INa is essential for SAN conduction, especially in fibrotic failing hearts. Our results reveal that not only cNav but nNav are also integral for preventing disease-induced failure in human SAN intranodal conduction. Disease-impaired nNav may underlie patient-specific SAN dysfunctions and should be considered to treat arrhythmias.Entities:
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Year: 2020 PMID： 31980605 PMCID： PMC6981137 DOI： 10.1038/s41467-019-14039-8
Source DB: PubMed Journal: Nat Commun ISSN： 2041-1723 Impact factor: 14.919