Literature DB >> 3197836

Rapid preparation of the nicotinic acetylcholine receptor for crystallization in detergent solution.

S Hertling-Jaweed1, G Bandini, A Müller-Fahrnow, V Dommes, F Hucho.   

Abstract

A novel rapid purification method for the nicotinic acetylcholine receptor from Torpedo electric tissue was developed. It allows preparation of 10 mg quantities of pure and stabile receptor protein within 2 days. This protein is used for crystallization attempts. Conditions are described which reproducibly yield crystals.

Mesh：

Substances：

Year:  1988        PMID： 3197836     DOI： 10.1016/0014-5793(88)81024-x

Source DB:  PubMed          Journal:  FEBS Lett        ISSN： 0014-5793            Impact factor:   4.124


  9 in total

1.  2-D- and 3-D-ordered structures of acetylcholine receptors.

Authors:  M Giersig; S Hertling-Jaweed; F Hucho
Journal:  J Protein Chem       Date:  1989-06

2.  The selectivity filter of a ligand-gated ion channel.

Authors:  F Hucho; R Hilgenfeld
Journal:  J Protein Chem       Date:  1989-06

3.  Role of glycosylation and membrane environment in nicotinic acetylcholine receptor stability.

Authors:  Corrie J B daCosta; Daniel E E Kaiser; John E Baenziger
Journal:  Biophys J       Date:  2004-12-30       Impact factor: 4.033

4.  Assessment of the functionality and stability of detergent purified nAChR from Torpedo using lipidic matrixes and macroscopic electrophysiology.

Authors:  Luis F Padilla-Morales; José O Colón-Sáez; Joel E González-Nieves; Orestes Quesada-González; José A Lasalde-Dominicci
Journal:  Biochim Biophys Acta       Date:  2015-10-08

5.  Effects of lipid-analog detergent solubilization on the functionality and lipidic cubic phase mobility of the Torpedo californica nicotinic acetylcholine receptor.

Authors:  Luis F Padilla-Morales; Claudio L Morales-Pérez; Pamela C De La Cruz-Rivera; Guillermo Asmar-Rovira; Carlos A Báez-Pagán; Orestes Quesada; José A Lasalde-Dominicci
Journal:  J Membr Biol       Date:  2011-09-16       Impact factor: 1.843

6.  Biophysical and ion channel functional characterization of the Torpedo californica nicotinic acetylcholine receptor in varying detergent-lipid environments.

Authors:  Guillermo A Asmar-Rovira; Aloysha M Asseo-García; Orestes Quesada; Michael A Hanson; Anchi Cheng; Carlos Nogueras; José A Lasalde-Dominicci; Raymond C Stevens
Journal:  J Membr Biol       Date:  2008-06-25       Impact factor: 1.843

7.  Uncovering the lipidic basis for the preparation of functional nicotinic acetylcholine receptor detergent complexes for structural studies.

Authors:  Orestes Quesada; Carol González-Freire; María Carla Ferrer; José O Colón-Sáez; Emily Fernández-García; Juan Mercado; Alejandro Dávila; Reginald Morales; José A Lasalde-Dominicci
Journal:  Sci Rep       Date:  2016-09-19       Impact factor: 4.379

Review 8.  Pursuing High-Resolution Structures of Nicotinic Acetylcholine Receptors: Lessons Learned from Five Decades.

Authors:  Manuel Delgado-Vélez; Orestes Quesada; Juan C Villalobos-Santos; Rafael Maldonado-Hernández; Guillermo Asmar-Rovira; Raymond C Stevens; José Antonio Lasalde-Dominicci
Journal:  Molecules       Date:  2021-09-23       Impact factor: 4.411

9.  Biophysical characterization dataset of native nicotinic acetylcholine receptor in lipid-like detergent complexes.

Authors:  Rafael Maldonado-Hernández; Orestes Quesada; José A Lasalde-Dominicci
Journal:  Data Brief       Date:  2020-08-27
  9 in total

北京卡尤迪生物科技股份有限公司 © 2022-2023.