| Literature DB >> 31978347 |
David P Nusinow1, John Szpyt2, Mahmoud Ghandi3, Christopher M Rose2, E Robert McDonald4, Marian Kalocsay2, Judit Jané-Valbuena3, Ellen Gelfand3, Devin K Schweppe2, Mark Jedrychowski2, Javad Golji4, Dale A Porter4, Tomas Rejtar4, Y Karen Wang4, Gregory V Kryukov3, Frank Stegmeier4, Brian K Erickson2, Levi A Garraway5, William R Sellers4, Steven P Gygi6.
Abstract
Proteins are essential agents of biological processes. To date, large-scale profiling of cell line collections including the Cancer Cell Line Encyclopedia (CCLE) has focused primarily on genetic information whereas deep interrogation of the proteome has remained out of reach. Here, we expand the CCLE through quantitative profiling of thousands of proteins by mass spectrometry across 375 cell lines from diverse lineages to reveal information undiscovered by DNA and RNA methods. We observe unexpected correlations within and between pathways that are largely absent from RNA. An analysis of microsatellite instable (MSI) cell lines reveals the dysregulation of specific protein complexes associated with surveillance of mutation and translation. These and other protein complexes were associated with sensitivity to knockdown of several different genes. These data in conjunction with the wider CCLE are a broad resource to explore cellular behavior and facilitate cancer research.Entities:
Keywords: CCLE; MSI; RNA/Protein correlation; TMT; cancer cell lines; microsatellite instability; protein expression; quantitative proteomics; systems biology
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Year: 2020 PMID： 31978347 PMCID： PMC7339254 DOI： 10.1016/j.cell.2019.12.023
Source DB: PubMed Journal: Cell ISSN： 0092-8674 Impact factor: 41.582