Literature DB >> 3197804

Vitamins and other metabolites in various sera commonly used for cell culturing.

H Baker1, B DeAngelis, O Frank.   

Abstract

Many cell culture media use different sera to enhance growth. We assayed vitamins and some related metabolites in different sera and identified the concentration of: thiamin, biotin, folates, riboflavin, pantothenates, nicotinates, vitamins B6, B12, A, E, C, and carotenes and some related metabolites: biopterins, free inositol, free and total choline, total carnitines in chicken, horse, rabbit, goat, pig, calf, newborn calf, fetal calf and human sera. Results indicate that vitamin and metabolite content of different sera vary. Such variations could produce fluctuant effects on cell culturings if the metabolite content of the serum is not documented.

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Year:  1988        PMID： 3197804     DOI： 10.1007/bf01939904

Source DB:  PubMed          Journal:  Experientia        ISSN： 0014-4754


  12 in total

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Authors:  J MOHBERG; M J JOHNSON
Journal:  J Natl Cancer Inst       Date:  1963-09       Impact factor: 13.506

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Authors:  I TOPLIN
Journal:  Cancer Res       Date:  1961-09       Impact factor: 12.701

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Authors:  C De Luca; C Carruthers; G L Tritsch
Journal:  Exp Cell Res       Date:  1966-09       Impact factor: 3.905

5.  Biopterin content of human and rat fluids and tissues determined protozoologically.

Authors:  H Baker; O Frank; C J Bacchi; S H Hunter
Journal:  Am J Clin Nutr       Date:  1974-11       Impact factor: 7.045

6.  Inhibition of human lymphocyte blastogenesis by C3: the role of serum in the tissue culture medium.

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Journal:  Immunology       Date:  1982-06       Impact factor: 7.397

7.  Blood vitamin and choline concentrations in healthy domestic cats, dogs, and horses.

Authors:  H Baker; S M Schor; B D Murphy; B DeAngelis; S Feingold; O Frank
Journal:  Am J Vet Res       Date:  1986-07       Impact factor: 1.156

8.  Assay for free and total choline activity in biological fluids and tissues of rats and man with Torulopsis pintolopessi.

Authors:  H Baker; O Frank; D J Tuma; A J Barak; M F Sorrell; S H Hutner
Journal:  Am J Clin Nutr       Date:  1978-03       Impact factor: 7.045

9.  The action of excess of vitamin A alcohol on the fine structure of rat dermal fibroblasts.

Authors:  M R Daniel; J T Dingle; A M Glauert; J A Lucy
Journal:  J Cell Biol       Date:  1966-09       Impact factor: 10.539

10.  Cultivation of mammalian cells in defined media with protein and nonprotein supplements.

Authors:  G M Healy; R C Parker
Journal:  J Cell Biol       Date:  1966-09       Impact factor: 10.539
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  12 in total

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5.  Vitamin B12 and hepatitis C: molecular biology and human pathology.

Authors:  W B Lott; S S Takyar; J Tuppen; D H Crawford; M Harrison; T P Sloots; E J Gowans
Journal:  Proc Natl Acad Sci U S A       Date:  2001-04-10       Impact factor: 11.205

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8.  Goat serum as an alternative to establish cell culture from Indian major carp, Cirrhinus mrigala.

Authors:  P K Nanda; P Swain; S K Nayak; S Dash; P Routray; S K Swain; B C Patra
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10.  Chemical, genetic and structural assessment of pyridoxal kinase as a drug target in the African trypanosome.

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