| Literature DB >> 31977277 |
Nathally Claudiane de Souza Santos1, Regiane Bertin de Lima Scodro2,3, Eloísa Gibin Sampiron2, Andressa Lorena Ieque2, Hayalla Corrêa de Carvalho1, Thais da Silva Santos1, Luciana Dias Ghiraldi Lopes3, Paula Aline Zanetti Campanerut-Sá2,3, Vera Lucia Dias Siqueira1,3, Katiany Rizzieri Caleffi-Ferracioli1,3, Jorge Juarez Vieira Teixeira1,2, Rosilene Fressatti Cardoso1,2,3.
Abstract
Minimum bactericidal concentration (MBC) assay is an accepted parameter for evaluating new antimicrobial agents, and it is frequently used as a research tool to provide a prediction of bacterial eradication. To the best of our knowledge, there is no standardization among researchers regarding the technique used to detect a drug's MBC in Mycobacterium tuberculosis. Thus, the aim of this systematic review is to discuss the available literature in determining a drug's MBC in M. tuberculosis, to find the most commonly used technique and standardize the process. A broad and rigorous literature search of three electronic databases (PubMed, Web of Knowledge, and LILACS) was performed according to the PRISMA statement. We considered studies that were published from January 1, 1990 to February 19, 2019. Google Scholar was also searched to increase the number of publications. We searched for articles using the MeSH terms "microbiological techniques," "Mycobacterium," "antibacterial agents." In addition, free terms were used in the search. The search yielded 6,674 publications. After filter application, 5,348 publications remained. Of these, we evaluated the full text of 187 publications. By applying the inclusion criteria, 69 studies were included in the present systematic review. In the literature analyzed, a great variety in the techniques used to determine a drug's MBC in M. tuberculosis was observed. The most common variability is related to the culture media used, culture incubation time, and the percentage of bacterial death for the drug to be considered as bactericidal. The most commonly used technique for drug's MBC determination was carried out using the drug's minimum inhibitory concentration (MIC) assay. Aliquots from prior MIC values were subcultured in Middlebrook agar and incubated for 4 weeks at 35°C for determining the colony forming unit (CFU) with relevance to detect 99.9% bacilli killed or reduction in 3 log10 viable bacilli.Entities:
Keywords: Mycobacterium; antibacterial agents; dependent concentration; microbiological technique; minimum bactericidal concentration; tuberculosis
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Year: 2020 PMID： 31977277 DOI： 10.1089/mdr.2019.0191
Source DB: PubMed Journal: Microb Drug Resist ISSN： 1076-6294 Impact factor: 3.431