Lipid Fingerprinting of Different Material Sources by UPLC-Q-Exactive Orbitrap/MS Approach and Their Zebrafish-Based Activities Comparison.

Lipids are considered to be critical contributors to nutrition, structural function, metabolic function, and other biological roles. They vary from one biological source to another. Here, the lipids from three common sources (soybean, egg yolk, and shrimp head) were comprehensively compared and characterized using the lipidomics approach, together with the UPLC-Q-Exactive Orbitrap/MS method. A total of 3027 lipid structures containing 778 fatty acids, 750 glycerolipids, 1283 glycerophospholipids, 200 sphingolipids, and 16 sterol lipids were first identified and quantified in these resources. The characteristic lipid species with significant differences among groups were determined by lipidomics analysis. Besides, the antithrombotic, antioxidant, and anti-inflammatory activities were evaluated based on the zebrafish model. The correlation between differential lipids and activities was also analyzed. Our comprehensive lipidomics profiling and bioactivities of lipids from different sources in vivo can provide evidence for their future applications.