Laís Moro1, Alessio Da Ros2, Renata Vieira da Mota3, Eduardo Purgatto4, Fulvio Mattivi2,5, Panagiotis Arapitsas2. 1. Deptartment of Food Science and Experimental Nutrition/FORC - Food Research Center, University of São Paulo, São Paulo, Brazil. lais.moro@gmail.com. 2. Department of Food Quality and Nutrition, Research and Innovation Centre Fondazione Edmund Mach, San Michele all'Adige, Italy. 3. Empresa de Pesquisa Agropecuária de Minas Gerais - EPAMIG, Núcleo Tecnológico Uva e Vinho, Caldas, Minas Gerais, Brazil. 4. Deptartment of Food Science and Experimental Nutrition/FORC - Food Research Center, University of São Paulo, São Paulo, Brazil. 5. Center Agriculture Food Environment, University of Trento, Trento, Italy.
Abstract
INTRODUCTION: Vitis labrusca L. grapes are largely cultivated in Brazil, but the tropical climate negatively affects the phenols content, especially anthocyanin. According to the projections of the incoming climatic changes, the climate of several viticulture zone might change to tropical. Therefore, researches are focusing on increasing grape phenols content; with methyl jasmonate application (MeJa) is considered a good alternative. OBJECTIVES: The aim was to investigate with an untargeted approach the metabolic changes caused by the MeJa pre-harvest application on two Vitis labrusca L. cultivars grapes, both of them grown in two Brazilian regions. METHODS: Isabel Precoce and Concord grapes cultivated under subtropical climate, in the south and southeast of Brazil, received MeJa pre-harvest treatment. Grape metabolome was extracted and analyzed with a MS based metabolomics protocol by UPLC-HRMS-QTOF. RESULTS: Unsupervised data analysis revealed a clear separation between the two regions and the two cultivars, while supervised data analysis revealed biomarkers between the MeJa treatment group and the control group. Among the metabolites positively affected by MeJa were (a) flavonoids with a high degree of methylation at the B-ring (malvidin and peonidin derivatives and isorhamentin) for Isabel Precoce grapes; (b) glucosides of hydroxycinnamates, gallocatechin, epigallocatechin and cis-piceid for Concord grapes; and (c) hydroxycinnamates esters with tartaric acid, and procyanidins for the Southeast region grapes. CONCLUSION: These results suggest that MeJa can be used as elicitor to secondary metabolism in grapes grown even under subtropical climate, affecting phenolic biosynthesis.
INTRODUCTION:Vitis labrusca L. grapes are largely cultivated in Brazil, but the tropical climate negatively affects the phenols content, especially anthocyanin. According to the projections of the incoming climatic changes, the climate of several viticulture zone might change to tropical. Therefore, researches are focusing on increasing grape phenols content; with methyl jasmonate application (MeJa) is considered a good alternative. OBJECTIVES: The aim was to investigate with an untargeted approach the metabolic changes caused by the MeJa pre-harvest application on two Vitis labrusca L. cultivars grapes, both of them grown in two Brazilian regions. METHODS: Isabel Precoce and Concord grapes cultivated under subtropical climate, in the south and southeast of Brazil, received MeJa pre-harvest treatment. Grape metabolome was extracted and analyzed with a MS based metabolomics protocol by UPLC-HRMS-QTOF. RESULTS: Unsupervised data analysis revealed a clear separation between the two regions and the two cultivars, while supervised data analysis revealed biomarkers between the MeJa treatment group and the control group. Among the metabolites positively affected by MeJa were (a) flavonoids with a high degree of methylation at the B-ring (malvidin and peonidin derivatives and isorhamentin) for Isabel Precoce grapes; (b) glucosides of hydroxycinnamates, gallocatechin, epigallocatechin and cis-piceid for Concord grapes; and (c) hydroxycinnamates esters with tartaric acid, and procyanidins for the Southeast region grapes. CONCLUSION: These results suggest that MeJa can be used as elicitor to secondary metabolism in grapes grown even under subtropical climate, affecting phenolic biosynthesis.
Authors: Luca Narduzzi; Jan Stanstrup; Fulvio Mattivi; Pietro Franceschi Journal: Food Addit Contam Part A Chem Anal Control Expo Risk Assess Date: 2018-10-23
Authors: Lloyd W Sumner; Alexander Amberg; Dave Barrett; Michael H Beale; Richard Beger; Clare A Daykin; Teresa W-M Fan; Oliver Fiehn; Royston Goodacre; Julian L Griffin; Thomas Hankemeier; Nigel Hardy; James Harnly; Richard Higashi; Joachim Kopka; Andrew N Lane; John C Lindon; Philip Marriott; Andrew W Nicholls; Michael D Reily; John J Thaden; Mark R Viant Journal: Metabolomics Date: 2007-09 Impact factor: 4.290
Authors: Kenneth Haug; Reza M Salek; Pablo Conesa; Janna Hastings; Paula de Matos; Mark Rijnbeek; Tejasvi Mahendraker; Mark Williams; Steffen Neumann; Philippe Rocca-Serra; Eamonn Maguire; Alejandra González-Beltrán; Susanna-Assunta Sansone; Julian L Griffin; Christoph Steinbeck Journal: Nucleic Acids Res Date: 2012-10-29 Impact factor: 16.971