Yijun Hu1,2, Yang Yu3, Zhanyun Bu4, Biyun Cun5, Yi Gong6, Dongli Li1, Jianhua Li1, Li Lu1, Guodong Li7, Ling Yuan1. 1. Department of Ophthalmology, The First Affiliated Hospital of Kunming Medical University, Kunming, China. 2. Aier School of Ophthalmology, Central South University, Changsha, China. 3. Department of Ophthalmology, The Second People's Hospital of Qujing, Qujing, China. 4. Department of Ophthalmology, Henan Provincial People's Hospital, Zhengzhou, China. 5. Clinical Skill Center, Kunming Medical University, Kunming, China. 6. Department of Physiology, Kunming Medical University, Kunming, China; and. 7. Department of Ophthalmology, The Second Affiliated Hospital of Nanchang University, Nanchang, China.
Abstract
PURPOSE: To investigate the levels of systemic heparanase, inflammatory markers, and coagulation factor activities in patients with retinal vein occlusion (RVO). METHODS: This prospective study included 18 patients with central RVO, 22 patients with branch RVO, and 40 patients with age-related cataract as the control group. Serum heparanase protein levels and activities were measured by ELISA and a heparan degrading enzyme assay kit, respectively. Serum levels of MMP-2, MMP-9, TLR-2, and TLR-4 were measured by ELISA kits. The activities of coagulation factors (V, VII, VIII, and IX) were determined with an autoanalyzer. The Mann-Whitney U test was used to compare the above parameters between patients with RVO and control subjects. The relationship between two of the above parameters was analyzed by Spearman's correlation. RESULTS: Patients with RVO had higher levels of systemic heparanase protein, heparanase activities, coagulation factors' (V, VIII, and IX) activities, MMP-2, MMP-9, TLR-2, and TLR-4 compared with the control group. Systemic heparanase levels were correlated with serum levels of MMP-2, MMP-9, TLR-2, TLR-4, and activities of coagulation factors VIII and IX. CONCLUSION: Increase of systemic heparanase in RVO is associated with activation of systemic inflammation and blood hypercoagulability.
PURPOSE: To investigate the levels of systemic heparanase, inflammatory markers, and coagulation factor activities in patients with retinal vein occlusion (RVO). METHODS: This prospective study included 18 patients with central RVO, 22 patients with branch RVO, and 40 patients with age-related cataract as the control group. Serum heparanase protein levels and activities were measured by ELISA and a heparan degrading enzyme assay kit, respectively. Serum levels of MMP-2, MMP-9, TLR-2, and TLR-4 were measured by ELISA kits. The activities of coagulation factors (V, VII, VIII, and IX) were determined with an autoanalyzer. The Mann-Whitney U test was used to compare the above parameters between patients with RVO and control subjects. The relationship between two of the above parameters was analyzed by Spearman's correlation. RESULTS:Patients with RVO had higher levels of systemic heparanase protein, heparanase activities, coagulation factors' (V, VIII, and IX) activities, MMP-2, MMP-9, TLR-2, and TLR-4 compared with the control group. Systemic heparanase levels were correlated with serum levels of MMP-2, MMP-9, TLR-2, TLR-4, and activities of coagulation factors VIII and IX. CONCLUSION: Increase of systemic heparanase in RVO is associated with activation of systemic inflammation and blood hypercoagulability.