Literature DB >> 31972224

Advanced approaches for elucidating structures of large RNAs using NMR spectroscopy and complementary methods.

Anita Kotar1, Hannah N Foley2, Kirk M Baughman2, Sarah C Keane3.   

Abstract

NMR spectroscopy is a key technique that has significantly advanced our understanding of RNA structure and dynamics. However, determination of large RNA structures by NMR spectroscopy remains a significant technical challenge. In this review, we highlight advances that facilitate NMR studies of large RNAs, including methods for sample preparation, isotope labeling strategies, and data acquisition. In addition, we review hybrid approaches that have been instrumental in the structure determination of large RNAs.
Copyright © 2020 Elsevier Inc. All rights reserved.

Keywords:  Hybrid methods; Isotopic labeling; NMR spectroscopy; RNA structure; Residual dipolar coupling; Segmental labeling

Year:  2020        PMID： 31972224     DOI： 10.1016/j.ymeth.2020.01.009

Source DB:  PubMed          Journal:  Methods        ISSN： 1046-2023            Impact factor:   3.608


  7 in total

1.  Nearest-Neighbor Effects Modulate loxP Spacer DNA Chemical Shifts and Guide Oligonucleotide Design for Nuclear Magnetic Resonance Studies.

Authors:  Nicole Wagner; Mark P Foster
Journal:  Biochemistry       Date:  2022-01-05       Impact factor: 3.321

Review 2.  Developments in solution-state NMR yield broader and deeper views of the dynamic ensembles of nucleic acids.

Authors:  Bei Liu; Honglue Shi; Hashim M Al-Hashimi
Journal:  Curr Opin Struct Biol       Date:  2021-04-06       Impact factor: 7.786

3.  An exon-biased biophysical approach and NMR spectroscopy define the secondary structure of a conserved helical element within the HOTAIR long non-coding RNA.

Authors:  Ainur Abzhanova; Alexander Hirschi; Nicholas J Reiter
Journal:  J Struct Biol       Date:  2021-03-20       Impact factor: 3.234

4.  Editorial for "Methods to characterize virus small RNAs and RNA structures".

Authors:  Gary Brewer; Mei-Ling Li; Blanton S Tolbert
Journal:  Methods       Date:  2020-10-16       Impact factor: 3.608

Review 5.  Isotope Labels Combined with Solution NMR Spectroscopy Make Visible the Invisible Conformations of Small-to-Large RNAs.

Authors:  Theodore K Dayie; Lukasz T Olenginski; Kehinde M Taiwo
Journal:  Chem Rev       Date:  2022-04-20       Impact factor: 72.087

Review 6.  Overview of the Nucleic-Acid Binding Properties of the HIV-1 Nucleocapsid Protein in Its Different Maturation States.

Authors:  Assia Mouhand; Marco Pasi; Marjorie Catala; Loussiné Zargarian; Anissa Belfetmi; Pierre Barraud; Olivier Mauffret; Carine Tisné
Journal:  Viruses       Date:  2020-09-29       Impact factor: 5.048

Review 7.  NMR Studies of Retroviral Genome Packaging.

Authors:  Patricia S Boyd; Janae B Brown; Joshua D Brown; Jonathan Catazaro; Issac Chaudry; Pengfei Ding; Xinmei Dong; Jan Marchant; Colin T O'Hern; Karndeep Singh; Canessa Swanson; Michael F Summers; Saif Yasin
Journal:  Viruses       Date:  2020-09-30       Impact factor: 5.048
  7 in total

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