Joseph Cichon1, Jordi Magrané2, Elina Shtridler3, Chao Chen3, Linlin Sun4, Guang Yang4, Wen-Biao Gan5. 1. Department of Anesthesiology and Critical Care, Penn Medicine, University of Pennsylvania, Philadelphia, PA, USA. 2. Feil Family Brain and Mind Research Institute, Weill Cornell Medical College, New York, NY, USA. 3. Molecular Neurobiology Program, Skirball Institute, Department of Physiology and Neuroscience, New York University School of Medicine, New York, NY, USA. 4. Department of Anesthesiology, Columbia University Medical Center, New York, NY, USA. 5. Molecular Neurobiology Program, Skirball Institute, Department of Physiology and Neuroscience, New York University School of Medicine, New York, NY, USA; Department of Anesthesiology, New York University School of Medicine, New York, NY, USA. Electronic address: Wenbiao.Gan@nyulangone.org.
Abstract
BACKGROUND: The genetically encoded calcium (Ca2+) sensor GCaMP6 has been widely used for imaging Ca2+ transients in neuronal somata, dendrites, and synapses. NEW METHOD: Here we describe five new transgenic mouse lines expressing GCaMP6F (fast) or GCaMP6S (slow) in the central and peripheral nervous system under the control of theThy1.2 promoter. RESULTS: These transgenic lines exhibit stable and layer-specific expression of GCaMP6 in multiple brain regions. They have several unique features compared to existing Thy1.2-GCaMP6 mice, including sparse expression of GCaMP6 in layer V pyramidal neurons of the cerebral cortex, motor neurons in the spinal cord, as well as sensory neurons in dorsal root ganglia (DRG). We further demonstrate that these mouse lines allow for robust detection of Ca2+ transients in neuronal somata and apical dendrites in the cerebral cortex of both anesthetized and awake behaving mice, as well as in DRG neurons. COMPARISON WITH EXISTING METHOD(S): These transgenic lines allows Ca2+ imaging of dendrites and somas of pyramidal neurons in specific cortical layers that is difficult to achieve with existing methods. CONCLUSIONS: These GCaMP6 transgenic lines thus provide useful tools for functional analysis of neuronal circuits in both central and peripheral nervous systems.
BACKGROUND: The genetically encoded calcium (Ca2+) sensor GCaMP6 has been widely used for imaging Ca2+ transients in neuronal somata, dendrites, and synapses. NEW METHOD: Here we describe five new transgenic mouse lines expressing GCaMP6F (fast) or GCaMP6S (slow) in the central and peripheral nervous system under the control of theThy1.2 promoter. RESULTS: These transgenic lines exhibit stable and layer-specific expression of GCaMP6 in multiple brain regions. They have several unique features compared to existing Thy1.2-GCaMP6 mice, including sparse expression of GCaMP6 in layer V pyramidal neurons of the cerebral cortex, motor neurons in the spinal cord, as well as sensory neurons in dorsal root ganglia (DRG). We further demonstrate that these mouse lines allow for robust detection of Ca2+ transients in neuronal somata and apical dendrites in the cerebral cortex of both anesthetized and awake behaving mice, as well as in DRG neurons. COMPARISON WITH EXISTING METHOD(S): These transgenic lines allows Ca2+ imaging of dendrites and somas of pyramidal neurons in specific cortical layers that is difficult to achieve with existing methods. CONCLUSIONS: These GCaMP6 transgenic lines thus provide useful tools for functional analysis of neuronal circuits in both central and peripheral nervous systems.
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