Socket preservation using eggshell-derived nanohydroxyapatite with platelet-rich fibrin as a barrier membrane: a new technique.

OBJECTIVES: Socket grafting is vital to prevent bone resorption after tooth extraction. Several techniques to prevent resorption have been described, and various bone graft substitutes have been developed and used with varying success. We conducted this pilot study to evaluate the performance of nanohydroxyapatite (nHA) derived from chicken eggshells in socket preservation.
MATERIALS AND METHODS: This was a prospective, single center, outcome assessor-blinded evaluation of 23 sockets (11 patients) grafted with nHA and covered with platelet-rich fibrin (PRF) membrane as a barrier. Bone width and radiographic bone density were measured using digital radiographs at 1, 12, and 24 weeks post-procedure. Postoperative histomorphometric and micro-computed tomography (CT) evaluation were performed. The study protocol was approved by the Institutional Ethics Committee.
RESULTS: All patients had uneventful wound healing without graft material displacement or leaching despite partial exposure of the grafted socket. Tissue re-epithelialized with thick gingival biotype (>3 mm). Width of the bone was maintained and radiographic density increased significantly with a trabecular pattern (73.91% of sockets) within 12 weeks. Histomorphometric analysis showed 56.52% Grade 3 bone formation and micro-CT analysis revealed newly formed bone with interconnecting trabeculae.
CONCLUSION: Use of a PRF membrane with nHA resulted in good bone regeneration in sockets. Use of a PRF membrane prevents periosteal-releasing incisions for primary closure, thereby facilitating the preservation of keratinized mucosa and gingival architecture. This technique, which uses eggshell-derived nHA and PRF membrane from the patient's own blood, is innovative and is free of disease transfer risks. nHA is a promising economic bone graft substitute for bone regeneration and reconstruction because of the abundant availability of eggshell waste as a raw material.

I. Introduction

Socket grafting is vital to prevent bone resorption1. Remodeling of alveolar bone is a continuous process in physiology and pathology, but alveolar bone is essential for the retention of teeth. Extraction of teeth leads to an edentulous space and loss of alveolar bone, function, and aesthetics23456. A systemic review of dimensional changes of the alveolar bone following extraction showed a mean reduction of 3.8 mm in width and 1.24 mm of height in the first six months after extraction7. Replacement of missing teeth is essential, but without the support of adequate healthy alveolar bone, teeth replacement is difficult8. Socket preservation attempts are therefore made to maintain bone to enable patients to have implant supported prosthesis in the future without the requirement for additional surgical procedures for augmentation1.

Socket preservation has been performed using different materials with varying success rates19101112. Materials used for grafting include autogenous bone, allograft, xenograft, and alloplasts, among others19. Various membranes have been used for wound closure, including polytetrafluoroethylene and bovine and porcine collagen matrices1. Graft materials enhance bone formation by promoting osteoconduction, osteoinduction, and osteogenesis13141516. However, no graft material to date is considered ideal. Recently, nanohydroxyapatite derived from chicken eggshell (nHA) was developed171819 and used as an innovative graft material for bone regeneration purposes202122, but none of these previous studies evaluated the performance of nHA and a platelet-rich fibrin (PRF) membrane in socket preservation20212223.

We therefore performed this pilot study to evaluate the histomorphometric and radiographic characteristics of bone regeneration when nHA and a PRF membrane were used for alveolar bone preservation and enhancement in the maxilla and mandible of humans to enable implant-supported restoration.

II. Materials and Methods

1. Study design

A total of 11 (eight male and three female) patients between the ages of 15 to 45 years who presented to the outpatient Department of Oral and Maxillofacial Surgery, Sibar Institute of Dental Sciences (Guntur, India) for extraction and willing to participate in the study protocol were enrolled during the period from June 2017 to June 2018. The Institutional Ethics Committee of Sibar Institute of Dental Sciences approved the study protocol (IEC-16/09/2014) and this study was registered with the Clinical Trials Registry-India (CTRI). Patients and patients' relatives were informed about the study protocol before enrollment and written informed consent was obtained. This was a prospective, single center, outcome assessor-blinded pilot study.

1) Sample size calculation

The sample size required for this pilot study was 10 patients but we recruited 11 patients involving one or more sockets per patient depending on the clinical scenario. Sample size was calculated with 80% power and a 5% alpha error based on a two-sided test. Total of 23 extraction sockets were assessed among the 11 patients.

2) Inclusion and exclusion criteria for patient selection

Patients with grossly decayed teeth, unrestorable teeth, those willing to undergo implant restoration, and those readily available for periodic recall were enrolled. Pregnant patients, patients with gross tooth mobility due to complete bone loss (more than 3/4 of root length), those in a vulnerable category or with a systemic illness (diabetes, thyroid, hypertension, etc.), and medically compromised patients were excluded from the study protocol to eliminate bias.

3) Materials

(1) Nanohydroxyapatite

nHA granules used for grafting were synthesized from natural calcium precursors obtained from chicken eggshell waste and produced using a rapid microwave processing technique1718. The nHA obtained in this manner is typical flower-like nanostructured HA19. It is composed of leaf-like flakes extending radially from the center with a width of 100 to 200 nm and length of 0.5 to 1 µm. nHA contains Ca, P, O, C, and Mg1819. Incorporation of Mg ions into the HA structure is important for the development of artificial bones19.

(2) Platelet-rich fibrin membrane

PRF membrane was prepared using autologous blood under standard aseptic precautions. Intravenous blood (5 mL) was drawn from the antecubital region using a sterile disposable syringe. Blood was centrifuged using a tabletop centrifuge for 10 minutes at 3,000 rpm immediately after collection. The resultant product comprised acellular plasma as the top layer, a PRF clot in the middle, and red blood cells at the bottom. Successful preparation of PRF depends on speedy blood collection and immediate centrifugation before clotting begins. PRF component was separated and molded into a thin sheet of approximately 1×1 cm using a sterile, wet piece of gauze.

2. Operative procedure

Extraction of the tooth or tooth root was performed atraumatically under local anesthesia. If necessary, curettage was performed (Fig. 1. A) and irrigation was done with Betadine and saline. Socket compression was not performed to prevent the collapse of bony walls. After hemostasis, nHA grafting was performed to the crestal level in all 23 sockets.(Fig. 1. B) PRF membrane was transferred over the grafted socket and tucked between buccal and lingual/palatal gingiva and stabilized using 3-0 black silk suture (figure of eight). The suture passed through the membrane to prevent slippage. PRF membrane prevented leaching of materials and periosteal-releasing incisions for primary closure.(Fig. 1. C) Postoperatively, all patients were administered antibiotics and analgesics for five days as a standard of care. Patients were recalled after 8 days for suture removal.

Fig. 1

A. Bone defect immediately after extraction with curette in place. B. Bone graft material placed till crestal level in the mandibular left first molar socket. C. Wound closure using platelet-rich fibrin membrane and figure of eight suture. D. Well healed wound after two weeks. E. Well-formed bone observed during exposure for implant bed preparation. F. Cone-beam computed tomography pre-extraction view with roots and bone defect marked in red circles. G. Bone formation (red circles) assessed before implant placement.

Implant bed was prepared using a trephine bur (3×10 mm) followed by a standard sequential drilling protocol for implant placement 24 weeks after grafting. Depending on initial stability, implants were loaded immediately or in a delayed manner using NobelParallel conical connection root form implants made by Nobel Biocare (Kloten, Sweden).

3. Clinical and radiographic evaluation

Wound healing was assessed prospectively.(Fig. 1. D) Patients were followed-up for 24 weeks until implant insertion.(Fig. 1. E) Preoperative cone-beam computed tomography (CBCT) revealed bony defects with roots.(Fig. 1. F) Bone width was measured using bone calipers and radiographic bone density was measured using digital intraoral peri-apical radiograph at week 1, 12, and 24 postoperatively. CBCT was obtained for planning and assessment before implant placement.(Fig. 1. G) Digora software (Digora for Windows 2.8.107.458 Network Client, Soredex Orion Corporation, Finland) was used to assess follow-up density changes and bone formation characteristics.(Fig. 2) Radiographic evaluation criteria were modified and adapted for assessment2425. Gridlines in digital IOPA were used as reference lines to assess bone height postoperatively for available bone for implant size selection. The assessor was blinded for the time duration of follow-up during radiographic evaluation.

Fig. 2

Intra-oral periapical radiographic images for assessment of bone formation and density using Digora software.

1) Micro-CT evaluation

During implant placement, a trephine biopsy (taken using a 3×10-mm trephine bur) harvested from the grafted site was subjected to histomorphometric and micro-CT evaluation.(Fig. 3) Micro-CT analysis was carried out using a PhoenixV/tome/Xs machine (GE Sensing & Inspection Technologies GmbH, Wunstorf, Germany).(Fig. 4. A–C) A series of microfocus CT images were acquired as x-ray images while progressively rotating the sample step by step through a 360° rotation at increments less than 1° per step.(Fig. 4. D) These projections were used to reconstruct volumetric data. The multiline configuration of the flat panel covered with a movable shield reduced scattering artifacts. A temperature-stabilized DXR500L detector (General Electric, Boston, MA, USA) with 3,072 pixels provided a voxel size of 1.6 µm for assessment and allowed scanning of objects with voxel sizes <2 µm.

Fig. 3

A. Thick tissue biotype observed after removal of the screw-retained abutment of an immediate-loaded implant following suture removal after 1 week. B. Trephined bone (3×10 mm size) harvested from the left mandibular first molar site 24 weeks after grafting (during implant bed preparation) for micro-computed tomography (CT) and histomorphometric analysis. C, D. Micro-CT sections of trephine bone showing intervening trabeculae.

Fig. 4

A–C. Micro-computed tomography (CT) assessment of trephined bone using PhoenixV/tome/Xs machine image analyzing software. D. Schematic presentation of micro-CT image acquisition process for analysis using the PhoenixV/tome/Xs machine.

2) Histomorphometric evaluation

After micro-CT analysis, sections were decalcified using 5% EDTA. After processing, specimens were embedded in a paraffin wax block and sliced with a microtome to a thickness of 5 µm for H&E staining.(Fig. 5) The core was cut transversely and interpreted using image analysis software by two oral and maxillofacial pathologists blinded to the procedure. Histology analysis criteria were modified and adopted for evaluation as follows: Grade 0, no bone formation; Grade 1, minimal bone formation; Grade 2, moderate bone formation; Grade 3, abundant bone formation; and Grade 4, exuberant bone formation at the grafted site26.

Fig. 5

A. Microphotograph of two (10 mm and 5 mm) trephine bone specimens showing intervening trabeculae with a rim of osteoblasts (arrows). B. Osteoid and fibrous connective tissue with plump lacunae with prominent osteocytes was visible (arrows). A, B. H&E staining (×10).

4. Statistical analysis

Results were tabulated using Microsoft Excel 2010 (Microsoft, Redmond, WA, USA). Data were summarized as means and percentages and analyzed by dependent t-tests using IBM SPSS Statistics software (ver. 20.0; IBM, Armonk, NY, USA). Kappa correlation was calculated to assess the degree of observer agreement for radiological assessment.

III. Results

1. Clinical observations

All patients (23 sockets) had uneventful wound healing. No graft material displacement or leaching was observed, even though the graft material was partially exposed. Tissue re-epithelialized entirely within 2 weeks with a thick gingival biotype (>3 mm). Radiographic analysis showed increased density with a trabecular pattern in 73.91% of sockets while in the remaining 26.09% of sockets, a ground glass appearance was observed.(Fig. 6) After 24 weeks, bone fill with well-preserved ridges was observed. Socket wall width was measured at the crestal bone level and was consistent from week 4 to week 24.(Tables 1, 2, 3) Graft particles were incorporated into the generated bony tissue and the augmented site was clinically indistinguishable from the original neighboring bony tissue. Many of these sites had successfully placed implants.

Fig. 6

Pattern of bone regeneration and distribution of these patterns.

Table 1

Socket preservation, changes in alveolar width at different time intervals, pattern of bone regeneration, and histopathology grading 24 weeks after bone grafting

No.	Tooth No. (FDI) of socket preservation	Alveolar width (mm)		Mean density after			Pattern of bone regeneration	Grade of bone formation based on Initial Final 1 wk 12 wk 24 wk histopathology1
		Initial	Final	1 wk	12 wk	24 wk
1	37	13	12	60.60	40.32	76.34	Trabecular	3
2	36	14	13	63.27	55.50	88.80	Trabecular	3
3	11	14	14	54.34	34.43	70.46	Ground glass	3
4	12	13	13	50.12	45.10	65.27	Ground glass	4
5	13	15	14	53.24	40.23	58.89	Ground glass	2
6	14	14	13	50.32	43.12	65.13	Ground glass	4
7	15	13	12	44.45	34.10	67.73	Trabecular	3
8	23	15	14	54.43	45.20	73.49	Trabecular	2
9	24	14	14	50.32	40.12	75.21	Trabecular	3
10	33	13	13	45.56	34.34	58.12	Ground glass	3
11	35	13	12.5	56.75	45.65	69.15	Ground glass	2
12	44	13	12.5	45.43	34.43	72.14	Trabecular	3
13	45	14	13	45.21	32.54	73.16	Trabecular	3
14	36	14.5	14.5	50.68	45.00	69.76	Trabecular	3
15	21	14	13.5	54.45	34.80	68.23	Trabecular	3
16	36	14.5	14	50.89	29.01	75.20	Trabecular	3
17	36	15	14	59.43	36.03	76.08	Trabecular	3
18	36	14	14	66.34	35.04	79.05	Trabecular	-
19	38	15	14.5	63.12	60.06	80.03	Trabecular	-
20	14	14	13.5	64.34	58.09	76.83	Trabecular	-
21	15	13	13	44.65	35.07	69.01	Trabecular	3
22	47	15	14	56.87	43.05	70.01	Trabecular	-
23	48	16	15	68.54	46.03	74.01	Trabecular	-

(FDI: Fédération Dentaire Internationale; FDI World Dental Federation)

1Histologic analysis graded on the following 5-point scale: 1) Grade 0, no bone formation; 2) Grade 1, minimal bone formation; 3) Grade 2, moderate bone formation; 4) Grade 3, abundant bone formation; 5) Grade 4, exuberant bone formation.

Table 2

Comparison of initial and final alveolar width (mm) scores by dependent t-test

Time points	Mean	SD	Mean Diff.	SD Diff.	% of change	Paired t	P-value
Initial	14.04	0.86
Final	13.48	0.80	0.57	0.43	4.02	6.2395	0.0001*

(SD: standard deviation, Diff.: difference)

*P<0.05

Table 3

Comparison of density scores 1 week, 12 weeks, and 24 weeks by dependent t-test

Time points	Mean	SD	Mean Diff.	SD Diff.	% of change	Paired t	P-value
1st wk	54.49	7.35
12th wk	41.19	8.26	13.31	7.17	24.42	8.9052	0.0001*
1st wk	54.49	7.35
24th wk	71.83	6.74	–17.34	6.32	–31.81	–13.1497	0.0001*
12th wk	41.19	8.26
24th wk	71.83	6.74	–30.65	8.29	–74.41	–17.7222	0.0001*

(SD: standard deviation, Diff.: difference)

*P<0.05.

2. Histomorphometric and micro-CT observations

Micro-CT analysis revealed newly formed bone with interconnecting trabeculae and H&E staining revealed thin trabeculae of woven bone. Plenty of cellular osteoid in the form of trabeculae with intervening loose relatively dense cellular fibrous tissue was observed. Trabeculae showed a rich anastomosing pattern with numerous enlarged lacunae containing prominent osteocytes. Numerous bundles of collagen fibers were also observed. Osteoid showed eosinophilic areas surrounded by a rim of osteoblasts.(Fig. 5) The graft material was in apposition with the osteoid. No osteoclasts or inflammatory cells were observed. Grade 2 bone formation was observed in 13.04% of grafted sockets, Grade 3 bone formation in 56.52% of grafted sockets, and Grade 4 bone formation in 8.70% of grafted sockets.(Fig. 7)

Fig. 7

Histological grade of bone regeneration and distribution of these grades.

IV. Discussion

1. General aspects

Socket preservation is essential for maintaining adequate alveolar bone height and width and enhancing bone regeneration. Alveolar bone is required to support the growth of thick soft tissue. Various materials and techniques have been used for socket preservation127. Selection of a socket preservation technique and graft material remains the choice of the clinician. Many factors such as the pattern of resorption (resorbable or non-resorbable), graft material origin (bovine, synthetic, coral, eggshell), material availability, economic factors, and understanding of the evidence for regeneration influence the choice of graft material141516.

The disadvantages associated with harvesting autogenous grafts have prompted research into novel materials for grafting14. An increasing number of synthetic alternatives are becoming available16. Improvements in technology have facilitated the synthesis of hydroxyapatite graft particles that are osteoinductive282930.

Synthetic HA is a calcium phosphate material that can have different densities, structures, and surface chemistries depending on its exact composition. These characteristics are responsible for the bone bonding properties, turnover rate, and longevity of the material in situ18. The efficacy of various HA materials in long-term ridge preservation has been proven. However, HA used in socket preservation should preserve existing bone and modulate early bone regeneration to facilitate implant osseointegration3132. Synthetic nHA does not require a donor site, and raw material is available in unlimited quantities2633. Control of particle size and inter-particulate spaces to mimic those of natural bone is possible during manufacturing171819.

2. Nanohydroxyapatite derived from chicken eggshell

We used nHA derived from chicken eggshell in the present study. Nanosynthetic HA has shown promising results for socket preservation, and our findings further support the utility of nanosynthetic HA for this purpose34. Histology sections from superficial to deeper cuts showed a consistent increase in newly formed bone with a decrease in the connective tissue area fraction. Histomorphometric findings indicated moderate to abundant bone formation within 24 weeks. Moreover, lower amounts of residual graft particles were observed, similar to the study of Goetz et al.35. Goetz et al.35 used NanoBone synthetic material for socket filling and reported nearly complete ossification with small residues of material after 4 months follow-up. NanoBone was osteoconductive based on its ability to promote bony ingrowth and initiate integration with newly formed bone. Thus total incorporation of generated bony tissue and mineral particles was achieved3435. New tissue formation in the healed socket was observed in approximately 75% of all examined specimens. Ultimately, the graft material should induce new bone formation34. New bone formation was evident in all samples and augmented sockets had an adequate morphology and density.

Preliminary experimental studies have also evaluated nano-sized ceramics as a promising class of graft substitute materials because of their osteo-integrative properties36. In these studies, nHA paste was used with or without an additional barrier membrane36. This paste promoted wound healing in the extraction sockets. Nanoparticles can hypothetically reduce the inflammatory response and support active osseous organization3637.

The nHA used in this study meets the characteristics of an ideal graft material noted by Gross31 in 1997. The material is available in granular/nanopowder form, which is easy to handle, and is not prone to infection. In this study, the grafted area was exposed to the oral cavity because we did not use primary closure or a barrier membrane other than a PRF membrane to prevent initial leaching of the material until it became cohesive with a blood clot. nHA is hydrophilic and sticks to the socket wall and settles down in the blood soon after filling. There is no possibility of disease transfer because the material is derived from chicken eggshell. Histology clearly showed the development of bone reminiscent of native bone. Mobility of the tooth has been shown to be maintained when this material is grafted into large cystic defects23, and it also supports implant prostheses successfully. Similar findings have been reported for synthetic grafts38 with clinically successful implants observed after five years38. Furthermore, bone density and volume have been shown to increase over time3839. As noted by Boyne40, synthetics act as a natural barrier for epithelial downgrowth into the socket. The ability to maintain the height and width of alveolar bone and soft tissue anatomy has distinct functional and esthetic advantages3340. We observed a significant increase in density from the week 1 to week 12 (P<0.05). Minimal bone loss (0.57±0.43 mm) was observed over 24 weeks compared to baseline, consistent with the literature1. Alveolar bone preservation and replacement therapy can be achieved using nHA without a collagen barrier membrane. nHA is therefore a promising graft material that can be produced in an environmentally-friendly manner1719.

3. Socket preservation technique and barrier membrane

We used nHA for socket preservation23. No cases of leaching or displacement of the graft material were observed and no periosteal-releasing incisions occurred. Use of an invasive method is recommended against at this point as any procedure that requires primary intention healing with the advancement of flaps can result in an increased inflammatory response and a decrease in vestibular depth, creating an unaesthetic scar41. The study by Fickl et al.42 showed that flap elevation results in the loss of more bone. This is because rupture of the periosteum and connective tissue insertion consequently reduces blood flow, causing lysis of osteocytes and necrosis of mineralized tissue42. This necrotic bone will gradually be removed through resorption by osteoclasts present in the periosteum4344. We overcame this problem of bone loss by not elevating the flap. Elevation also negatively affects the esthetics of the ridge and papilla by altering the mucogingival line position2. We used PRF membrane to cover the graft material during the initial period; we consider this an essential step for successful grafting with the existing attached gingiva and future success with implant restoration.

Many current socket preservation techniques use a barrier membrane for primary closure, but there are often difficulties in adoption of the membrane, and a second surgical procedure is required to remove the membrane45. In general, membrane use is time-consuming and expensive454647. Murphy4546 reported exposure of non-resorbable membrane in 87% of cases, leading to infection, swelling, sloughing, and recession. We did not encounter these complications using PRF membrane to cover the graft material. Synthetic grafts do not require a second surgical procedure, nor do they elicit an inflammatory reaction, which is usually seen with a barrier membrane47. Formation of dense lamellar bone at the grafted area has been observed20. Socket grafting eliminates or reduces the incidence of dry socket33; consistent with this, no cases of dry socket were encountered in our study. Moreover, invasive procedures like guided bone regeneration and sinus floor elevation are not needed when socket grafting is adopted and planned properly before extraction41.

The PRF membrane in our study acted as a cover for graft material and as a barrier membrane for guided bone regeneration. The effect of PRF as a barrier membrane for bone formation and soft tissue healing may be limited, but PRF and nHA may interact additively or synergistically. The usefulness of PRF in the maxillofacial area is expanding48. Our study design did not allow us to distinguish between the effects of nHA and PRF, although histomorphometry analysis at the bottom of the socket and middle of the socket showed similar bone formation characteristics as that in the crestal area. In previous studies, nHA alone enhanced bone formation in cystic defects2349. Even after disintegration of the PRF membrane, material leaching was not observed. Alveolar bone loss occurs if socket preservation techniques are not employed1. We therefore did not include a control group in this preliminary study as this study is part of an ongoing study protocol to delineate the histomorphometric and micro-CT characteristics of bone formation in relation to radiography; previous studies have used only radiography (IOPA and CBCT/CT scan) for assessment of cystic defect grafting2349.

Grafting prevents hematoma and infection. Manipulation of graft material is easy because of the hydrophilic nature of nHA. It can be used as putty when mixed with a drop of saline or native blood or serum from the PRF test-tube. Its ease of use, absence of disease transfer risk, ready availability in large quantities, and environment-friendly production make nHA an economic graft substitute material for enhancement of bone regeneration and reconstruction. Application of nHA in grafted sockets supports implant restoration, indicating osseointegration.

4. Future research work

Advances in tissue engineering techniques are likely to provide novel biomaterials for everyday clinical use41. Long-term follow-up data are mandatory to evaluate the presence of grafted particles that could eventually interfere with the longevity of the implant. We are currently performing a study to further elucidate the histochemical and morphometric nature of the bone formed, as well as assess the resorption kinetics of nHA. The results of the current pilot study suggest that multicenter randomized controlled clinical trials with long-term implant restoration follow-up are warranted. Various grafting materials are available for purchase. Cost varies according to the manufacturer and depends on many factors such as the raw material source and processing technique involved14163132. Comparison of the costs of grafting is beyond the scope of this study.

Limitations of this pilot study are our small sample size and the fact that socket morphology, width, and presence of buccal/lingual cortical walls were not taken into account in our analyses.

V. Conclusion

Sockets preserved with nHA showed good bone regeneration. Use of nHA and a PRF membrane as a barrier can assist the healing process and provide better bone quality while preserving alveolar bone. Micro-CT provides good insight into bone healing and bone quality in three dimensions compared to conventional histopathology. The technique described here does not require primary closure, thereby facilitating the preservation of keratinized mucosa and gingival architecture. nHA derived from chicken eggshell is a promising novel bone graft substitute.