Gileade P Freitas1, Helena B Lopes1, Alann T P Souza1, Paula G F P Oliveira1, Adriana L G Almeida1, Paulo G Coelho2,3, Fernanda U Ferreira4, Dimas T Covas4,5, Marcio M Beloti6, Adalberto L Rosa1. 1. Department of Oral & Maxillofacial Surgery & Periodontology, School of Dentistry of Ribeirão Preto, University of São Paulo, SP, Brazil. 2. Department of Biomaterials, New York University College of Dentistry, NY 10010, USA. 3. Hansjörg Wyss Department of Plastic Surgery, New York University School of Medicine, NY 10016, USA. 4. Center for Cell-Based Research, Regional Blood Center of Ribeirão Preto, School of Medicine of Ribeirão Preto, University of São Paulo, SP, Brazil. 5. Department of Clinical Medicine, School of Medicine of Ribeirão Preto, University of São Paulo, SP, Brazil. 6. Department of Basic & Oral Biology, School of Dentistry of Ribeirão Preto, University of São Paulo, SP, Brazil.
Abstract
Aim: The aim of this study was to investigate the effect of local injection of osteoblasts differentiated from bone marrow (BM-OB) or adipose tissue (AT-OB) mesenchymal stromal cells on bone tissue formation. Materials & methods: Defects were created in rat calvaria and injected with BM-OB or AT-OB and phosphate-buffered saline without cells were injected as control. Bone formation was evaluated 4 weeks postinjection. Results: Injection of BM-OB or AT-OB resulted in higher bone formation than that obtained with control. The bone tissue induced by cell injections exhibited similar mechanical properties as those of pristine calvarial bone, and its molecular cues suggested the occurrence of a remodeling process. Conclusion: Results of this study demonstrated that cell therapy with osteoblasts induced significant bone formation that exhibited the same quality as that of pre-existent bone.
Aim: The aim of this study was to investigate the effect of local injection of osteoblasts differentiated from bone marrow (BM-OB) or adipose tissue (AT-OB) mesenchymal stromal cells on bone tissue formation. Materials & methods: Defects were created in rat calvaria and injected with BM-OB or AT-OB and phosphate-buffered saline without cells were injected as control. Bone formation was evaluated 4 weeks postinjection. Results: Injection of BM-OB or AT-OB resulted in higher bone formation than that obtained with control. The bone tissue induced by cell injections exhibited similar mechanical properties as those of pristine calvarial bone, and its molecular cues suggested the occurrence of a remodeling process. Conclusion: Results of this study demonstrated that cell therapy with osteoblasts induced significant bone formation that exhibited the same quality as that of pre-existent bone.
Authors: Gileade P Freitas; Helena B Lopes; Alann T P Souza; Maria Paula O Gomes; Georgia K Quiles; Jonathan Gordon; Coralee Tye; Janet L Stein; Gary S Stein; Jane B Lian; Marcio M Beloti; Adalberto L Rosa Journal: Gene Ther Date: 2021-03-08 Impact factor: 4.184