A Vijaykumar1, M Mina2. 1. Department of Craniofacial Sciences School of Dental Medicine, University of Connecticut, Farmington, CT, United States. 2. Department of Craniofacial Sciences School of Dental Medicine, University of Connecticut, Farmington, CT, United States. Electronic address: mina@uchc.edu.
Abstract
OBJECTIVE: In the present study, we compared the in vitro osteogenic and dentinogenic potential of pulp cells from incisors and molars. DESIGN: Primary pulp cultures were established from DSPP-Cerulean/DMP1-Cherry and BSP-GFPtpz reporter mouse lines and processed for various assays. RESULTS: Our results showed marked differences in dentinogenic and osteogenic potentials of primary cultures from unerupted molars and incisors isolated from 5 to 7 days old pups. While primary cultures from both incisors and molars differentiated into odontoblasts and osteoblasts, cultures from molars differentiated into more DSPP-Cerulean + cells (∼5.5 %) compared to incisor cultures (∼0.7 %) at Day 14 and appear to be more committed to the odontogenic lineage. On the other hand, cultures from incisors show more differentiation into BSP-GFPtpz + cells (∼25 %) compared to molar cultures (∼16 %) and were more committed to the osteogenic lineage. Data were analyzed by Student's t-test and statistical significance was set at P ≤ 0.05. CONCLUSION: Since in the dental pulp, MSCs are the primary source of progenitors capable of giving rise to osteoblasts and odontoblasts, our results provide evidence for differences in the commitment of MSCs in molars and incisors to the odontogenic and osteogenic lineages, respectively.
OBJECTIVE: In the present study, we compared the in vitro osteogenic and dentinogenic potential of pulp cells from incisors and molars. DESIGN: Primary pulp cultures were established from DSPP-Cerulean/DMP1-Cherry and BSP-GFPtpz reporter mouse lines and processed for various assays. RESULTS: Our results showed marked differences in dentinogenic and osteogenic potentials of primary cultures from unerupted molars and incisors isolated from 5 to 7 days old pups. While primary cultures from both incisors and molars differentiated into odontoblasts and osteoblasts, cultures from molars differentiated into more DSPP-Cerulean + cells (∼5.5 %) compared to incisor cultures (∼0.7 %) at Day 14 and appear to be more committed to the odontogenic lineage. On the other hand, cultures from incisors show more differentiation into BSP-GFPtpz + cells (∼25 %) compared to molar cultures (∼16 %) and were more committed to the osteogenic lineage. Data were analyzed by Student's t-test and statistical significance was set at P ≤ 0.05. CONCLUSION: Since in the dental pulp, MSCs are the primary source of progenitors capable of giving rise to osteoblasts and odontoblasts, our results provide evidence for differences in the commitment of MSCs in molars and incisors to the odontogenic and osteogenic lineages, respectively.
Authors: Yvonne Wy Pang; Jifan Feng; Felipe Daltoe; Robert Fatscher; Eileen Gentleman; Molly M Gentleman; Paul T Sharpe Journal: J Bone Miner Res Date: 2015-10-21 Impact factor: 6.741
Authors: Audrey Rakian; Wu-Chen Yang; Jelica Gluhak-Heinrich; Yong Cui; Marie A Harris; Demitri Villarreal; Jerry Q Feng; Mary Macdougall; Stephen E Harris Journal: Int J Oral Sci Date: 2013-06-28 Impact factor: 6.344