Jovan T Juloski1, Aleksandar Rakic2, Vladica V Ćuk3, Vladimir M Ćuk4, Srđan Stefanović5, Dragica Nikolić6, Saša Janković7, Alexander M Trbovich8, Silvio R De Luka9. 1. Zvezdara Medical University Center, Surgery Clinic "Nikola Spasić", Dimitrija Tucovica 161, 11000 Belgrade, Serbia. Electronic address: jovan.juloski@gmail.com. 2. Department of Pathological Physiology, Faculty of Medicine, University of Belgrade, Dr Subotica 1, 11000 Belgrade, Serbia. Electronic address: aleksandar.rakic@med.bg.ac.rs. 3. Zvezdara Medical University Center, Surgery Clinic "Nikola Spasić", Dimitrija Tucovica 161, 11000 Belgrade, Serbia. Electronic address: vladicacuk@gmail.com. 4. Zvezdara Medical University Center, Surgery Clinic "Nikola Spasić", Dimitrija Tucovica 161, 11000 Belgrade, Serbia. Electronic address: cuk_cusa@yahoo.com. 5. Institute of Meat Hygiene and Technology, Kaćanskog 13, 11000 Belgrade, Serbia. Electronic address: srdjan.stefanovic@inmes.rs. 6. Institute of Meat Hygiene and Technology, Kaćanskog 13, 11000 Belgrade, Serbia. Electronic address: dragica.nikolic@inmes.rs. 7. Institute of Meat Hygiene and Technology, Kaćanskog 13, 11000 Belgrade, Serbia. Electronic address: sasa.jankovic@inmes.rs. 8. Department of Pathological Physiology, Faculty of Medicine, University of Belgrade, Dr Subotica 1, 11000 Belgrade, Serbia. Electronic address: alexander.m.trbovich@gmail.com. 9. Department of Pathological Physiology, Faculty of Medicine, University of Belgrade, Dr Subotica 1, 11000 Belgrade, Serbia. Electronic address: silvio.de-luka@med.bg.ac.rs.
Abstract
BACKGROUND: Trace elements have important influence on body function primarily because of the vital role they have in many physiological processes. Their alterations have been found in many disorders, including cancer. It has been well known for decades that disturbances in elemental concentration may lead to cell damaging, DNA injuries and imbalance in oxidative burden. Our study tried to determine the difference of trace elements concentrations between colorectal adenocarcinoma and adjacent healthy intestinal tissue. METHODS: 59 subjects participated in this study. Healthy colon mucosa samples and colon tumor tissue samples were obtained from patients previously diagnosed with colon carcinoma by standard diagnostic procedures. Analysis of the elements was performed by inductively coupled plasma mass spectrometry (ICP-MS). RESULTS: The results showed that Na, K, Mg, Ca, Cu, Zn, Se, Mn, Cd, Cr and Hg significantly differ between malignant tissue of colorectal cancer (CRC) and adjacent healthy bowel tissue. We have, also, found that Cu/Zn tissue ratio was significantly higher in CRC compared to a healthy tissue and that patients with higher CRC stages had also significantly higher ratio. CONCLUSIONS: Since this is the first such study in Balkan region, we assume that results of our study could be a good indicator of elemental alterations in colorectal cancer of Balkan population, due to similarity in lifestyle, dietary intake, pollution and exposure to toxic elements.
BACKGROUND: Trace elements have important influence on body function primarily because of the vital role they have in many physiological processes. Their alterations have been found in many disorders, including cancer. It has been well known for decades that disturbances in elemental concentration may lead to cell damaging, DNA injuries and imbalance in oxidative burden. Our study tried to determine the difference of trace elements concentrations between colorectal adenocarcinoma and adjacent healthy intestinal tissue. METHODS: 59 subjects participated in this study. Healthy colon mucosa samples and colon tumor tissue samples were obtained from patients previously diagnosed with colon carcinoma by standard diagnostic procedures. Analysis of the elements was performed by inductively coupled plasma mass spectrometry (ICP-MS). RESULTS: The results showed that Na, K, Mg, Ca, Cu, Zn, Se, Mn, Cd, Cr and Hg significantly differ between malignant tissue of colorectal cancer (CRC) and adjacent healthy bowel tissue. We have, also, found that Cu/Zn tissue ratio was significantly higher in CRC compared to a healthy tissue and that patients with higher CRC stages had also significantly higher ratio. CONCLUSIONS: Since this is the first such study in Balkan region, we assume that results of our study could be a good indicator of elemental alterations in colorectal cancer of Balkan population, due to similarity in lifestyle, dietary intake, pollution and exposure to toxic elements.
Authors: Julian Markovich Rozenberg; Margarita Kamynina; Maksim Sorokin; Marianna Zolotovskaia; Elena Koroleva; Kristina Kremenchutckaya; Alexander Gudkov; Anton Buzdin; Nicolas Borisov Journal: Biomedicines Date: 2022-05-05