Marian R Raouf1, Mohamed Sayed2, Hazim A Rizk3, Noha Anwar Hassuna4. 1. Minia University, Minia, Egypt. mar_rrs@yahoo.com. 2. Minia University, Minia, Egypt. mohammedsayed1973@yahoo.com. 3. Minia University, Minia, Egypt. dr_rizk_hazem@hotmail.com. 4. Minia University, Minia, Egypt. rn2623@yahoo.com.
Abstract
INTRODUCTION: Surgical wound infection is a serious problem, especially with metallo-beta lactamases (MBLs)- producing gram-negative bacteria as Pseudomonas aeruginosa. The main objective of this work was to evaluate for the first time in Minia- Upper Egypt, the incidence of imipenem-resistant Pseudomonas aeruginosa infection of surgical wounds particularly that mediated by MBL production. METHODOLOGY: P. aeruginosa was isolated from infected wounds by swabs and underwent full microbiological identification and Antibiotic Susceptibility testing. MBL production was tested by E-test and PCR was used for imipenemase (blaIMP) and Verona integron-encoded metallo-beta-lactamase (blaVIM) gene detection. RESULTS: Out of 200 pus samples collected from surgical site infections, P. aeruginosa had the prevalence rate of 35%. Imipenem resistance was found in 28.57% of the isolated Pseudomonas aeruginosa. The prevalence of MBL-producing isolates among Imipenem-resistant P. aeruginosa (IRPA) was 85 % by phenotypic method with 29% of them harboring blaVIM gene. High resistance rates to other classes of antibiotics were reported among the isolates with multi-drug resistance (MDR) detected in 97.3% of the isolates. CONCLUSION: To our knowledge, this is the first report in Minia, Upper Egypt describing the relatively high incidence of IRPA in infected surgical wounds with MBLs involved in the majority of isolates. Copyright (c) 2018 Noha Anwar Hassuna, Marian Raouf, Mohamed Sayed, Hazim Rizk.
INTRODUCTION: Surgical wound infection is a serious problem, especially with metallo-beta lactamases (MBLs)- producing gram-negative bacteria as Pseudomonas aeruginosa. The main objective of this work was to evaluate for the first time in Minia- Upper Egypt, the incidence of imipenem-resistant Pseudomonas aeruginosainfection of surgical wounds particularly that mediated by MBL production. METHODOLOGY:P. aeruginosa was isolated from infected wounds by swabs and underwent full microbiological identification and Antibiotic Susceptibility testing. MBL production was tested by E-test and PCR was used for imipenemase (blaIMP) and Verona integron-encoded metallo-beta-lactamase (blaVIM) gene detection. RESULTS: Out of 200 pus samples collected from surgical site infections, P. aeruginosa had the prevalence rate of 35%. Imipenem resistance was found in 28.57% of the isolated Pseudomonas aeruginosa. The prevalence of MBL-producing isolates among Imipenem-resistant P. aeruginosa (IRPA) was 85 % by phenotypic method with 29% of them harboring blaVIM gene. High resistance rates to other classes of antibiotics were reported among the isolates with multi-drug resistance (MDR) detected in 97.3% of the isolates. CONCLUSION: To our knowledge, this is the first report in Minia, Upper Egypt describing the relatively high incidence of IRPA in infected surgical wounds with MBLs involved in the majority of isolates. Copyright (c) 2018 Noha Anwar Hassuna, Marian Raouf, Mohamed Sayed, Hazim Rizk.