Soledad Alcántara1,2,3, Alfredo Garcia-Alix4,5,6,7, Rafael Balada1, Cristian Tebé8, Marisol León9, Gemma Arca9,10,11, Miguel Alsina9, Alba-Aina Castells1,12,2. 1. Neural Development Lab, Departament de Patologia i Terapèutica Experimental, l'Hospitalet de Llobregat, Barcelona, Spain. 2. Neuropatologia, IDIBELL, Barcelona, Spain. 3. Pathophysiology of Nervous System Disease, Institut de Neurociències, Universitat de Barcelona, Barcelona, Spain. 4. NeNe Foundation, Madrid, Spain. agarciaalix@sjdhospitalbarcelona.org. 5. Institut de Recerca Pediàtrica, Hospital Sant Joan de Déu, Barcelona, Spain. agarciaalix@sjdhospitalbarcelona.org. 6. Departament de Cirurgia i Especialitats Medicoquirúrgiques, University of Barcelona, Barcelona, Spain. agarciaalix@sjdhospitalbarcelona.org. 7. CIBERER. U724, Madrid, Spain. agarciaalix@sjdhospitalbarcelona.org. 8. Statistical Assessment Service, IDIBELL, l'Hospitalet de Llobregat, Barcelona, Spain. 9. Department of Neonatology, Hospital Casa de Maternitat, Barcelona, Spain. 10. IDIBAPS, Institut Clinic de Ginecologia, Obstetricia i Neonatologia, Barcelona, Spain. 11. NeNe Foundation, Madrid, Spain. 12. Neurogenètica i Medicina Molecular, Fundació Sant Joan de Déu, Esplugues de Llobregat, Spain.
Abstract
BACKGROUND: We aimed to assess whether a gene expression assay provided insights for understanding the heterogeneity among newborns affected by neonatal encephalopathy (NE). METHODS: Analysis by RT-qPCR of the mRNA expression of candidate genes in whole blood from controls (n = 34) and NE (n = 24) patients at <6, 12, 24, 48, 72 and 96 h of life, followed by determination of differences in gene expression between conditions and correlation with clinical variables. RESULTS: During the first 4 days of life, MMP9, PPARG, IL8, HSPA1A and TLR8 were more expressed and CCR5 less expressed in NE patients compared to controls. MMP9 and PPARG increased and CCR5 decreased in moderate/severe NE patients compared to mild. At 6-12 h of life, increased IL8 correlated with severe NE and death, decreased CCR5 correlated with chorioamnionitis and increased HSPA1A correlated with expanded multiorgan dysfunction, severe NE and female sex. CONCLUSIONS: MMP9, PPARG and CCR5 mRNA expression within first days of life correlates with the severity of NE. At 6-12 h, IL8 and HSPA1A are good reporters of clinical variables in NE patients. HSPA1A may have a role in the sexual dimorphism observed in NE. CCR5 is potentially involved in the link between severe NE and chorioamnionitis.
BACKGROUND: We aimed to assess whether a gene expression assay provided insights for understanding the heterogeneity among newborns affected by neonatal encephalopathy (NE). METHODS: Analysis by RT-qPCR of the mRNA expression of candidate genes in whole blood from controls (n = 34) and NE (n = 24) patients at <6, 12, 24, 48, 72 and 96 h of life, followed by determination of differences in gene expression between conditions and correlation with clinical variables. RESULTS: During the first 4 days of life, MMP9, PPARG, IL8, HSPA1A and TLR8 were more expressed and CCR5 less expressed in NEpatients compared to controls. MMP9 and PPARG increased and CCR5 decreased in moderate/severe NEpatients compared to mild. At 6-12 h of life, increased IL8 correlated with severe NE and death, decreased CCR5 correlated with chorioamnionitis and increased HSPA1A correlated with expanded multiorgan dysfunction, severe NE and female sex. CONCLUSIONS:MMP9, PPARG and CCR5 mRNA expression within first days of life correlates with the severity of NE. At 6-12 h, IL8 and HSPA1A are good reporters of clinical variables in NEpatients. HSPA1A may have a role in the sexual dimorphism observed in NE. CCR5 is potentially involved in the link between severe NE and chorioamnionitis.
Authors: Joanne O Davidson; Guido Wassink; Lotte G van den Heuij; Laura Bennet; Alistair J Gunn Journal: Front Neurol Date: 2015-09-14 Impact factor: 4.003
Authors: Agata Tarkowska; Wanda Furmaga-Jabłońska; Jacek Bogucki; Janusz Kocki; Ryszard Pluta Journal: J Clin Med Date: 2022-06-07 Impact factor: 4.964