Yu Ra Lee1,2, Haksoon Kim1,3, Bark Lynn Lew4, Woo Young Sim4, Jeongae Lee1, Han Bin Oh3, Jongki Hong5,6, Bong Chul Chung7,8. 1. Molecular Recognition Research Center, Korea Institute of Science and Technology, Seoul, 02792, Korea. 2. KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Seoul, 02447, Korea. 3. Department of Chemistry, Sogang University, Seoul, 04107, Korea. 4. Department of Dermatology, Kyung Hee University Hospital at Gangdong, Kyung Hee University, Seoul, 05278, Korea. 5. KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Seoul, 02447, Korea. jhong@khu.ac.kr. 6. College of Pharmacy, Kyung Hee University, Seoul, 02447, Korea. jhong@khu.ac.kr. 7. Molecular Recognition Research Center, Korea Institute of Science and Technology, Seoul, 02792, Korea. bcc0319@kist.re.kr. 8. KHU-KIST Department of Converging Science and Technology, Kyung Hee University, Seoul, 02447, Korea. bcc0319@kist.re.kr.
Abstract
INTRODUCTION: Alopecia areata is a well-known autoimmune disease affecting humans. Polyamines are closely associated with proliferation and inflammation, and steroid hormones are involved in immune responses. Additionally, bile acids play roles in immune homeostasis by activating various signaling pathways; however, the roles of these substances and their metabolites in alopecia areata remain unclear. OBJECTIVES: In this study, we aimed to identify differences in metabolite levels in urine samples from patients with alopecia areata and healthy controls. METHODS: To assess polyamine, androgen, and bile acid concentrations, we performed high-performance liquid chromatography-tandem mass spectrometry. RESULTS: Our results showed that spermine and dehydroepiandrosterone levels differed significantly between male patients and controls, whereas ursodeoxycholic acid levels were significantly higher in female patients with alopecia areata than in controls. CONCLUSION: Our findings suggested different urinary polyamine, androgen, and bile acid concentrations between alopecia areata patients and normal controls. Additionally, levels of endogenous substances varied according to sex, and this should be considered when developing appropriate treatments and diagnostic techniques. Our findings improve our understanding of polyamine, androgen, and bile acid profiles in patients with alopecia areata and highlight the need to consider sex-related differences.
INTRODUCTION:Alopecia areata is a well-known autoimmune disease affecting humans. Polyamines are closely associated with proliferation and inflammation, and steroid hormones are involved in immune responses. Additionally, bile acids play roles in immune homeostasis by activating various signaling pathways; however, the roles of these substances and their metabolites in alopecia areata remain unclear. OBJECTIVES: In this study, we aimed to identify differences in metabolite levels in urine samples from patients with alopecia areata and healthy controls. METHODS: To assess polyamine, androgen, and bile acid concentrations, we performed high-performance liquid chromatography-tandem mass spectrometry. RESULTS: Our results showed that spermine and dehydroepiandrosterone levels differed significantly between male patients and controls, whereas ursodeoxycholic acid levels were significantly higher in female patients with alopecia areata than in controls. CONCLUSION: Our findings suggested different urinary polyamine, androgen, and bile acid concentrations between alopecia areatapatients and normal controls. Additionally, levels of endogenous substances varied according to sex, and this should be considered when developing appropriate treatments and diagnostic techniques. Our findings improve our understanding of polyamine, androgen, and bile acid profiles in patients with alopecia areata and highlight the need to consider sex-related differences.
Entities:
Keywords:
Alopecia areata; Androgen; Bile acid; Immune; Polyamine
Authors: K Nakamura; M Yoneda; S Yokohama; K Tamori; Y Sato; K Aso; M Aoshima; T Hasegawa; I Makino Journal: J Gastroenterol Hepatol Date: 1998-05 Impact factor: 4.029
Authors: Meng-Lei Zhu; Pearl Bakhru; Bridget Conley; Jennifer S Nelson; Meghan Free; Aaron Martin; Joshua Starmer; Elizabeth M Wilson; Maureen A Su Journal: Nat Commun Date: 2016-04-13 Impact factor: 14.919