Sicong Jiang1, Yanhua Wang1, Yan Xiong1, Yanjun Feng1, Jianjun Tang1,2, Rongfeng Song1. 1. Department of Gastroenterology, Cancer Hospital of Jiangxi Province Nanchang, Jiangxi, China. 2. Department of Oncology, The Fifth Affiliated Hospital of Sun Yat-sen University Zhuhai, Guangdong, China.
Abstract
BACKGROUND: ZBED1 (zinc finger BED-type containing 1) is a transcription factor. However, its expression, role and clinical significance in cancer are unclear. The purpose of this study is to investigate the expression of ZBED1 and its effect on the proliferation of gastric cancer (GC). METHODS: Quantitative PCR was used to detect the mRNA level of ZBED1 in GC tissues and normal gastric tissues. Proliferation and colony formation assays were conducted when ZBED1 was expressed ectopically or silenced by constructed vectors. Moreover, chemotherapy-drug induced apoptosis rates were examined by flow cytometry when ZBED1 was expressed ectopically or silenced. RESULTS: The mRNA level of ZBED1 was significantly elevated in 10 out of 11 cases of GC tumor tissue specimens. Results of our analysis derived from a public clinical microarray database suggest that a high expression of ZBED1 predicts a poor outcome. ZBED1 promotes cell proliferation and colony formation. Moreover, ZBED1 decreased the chemosensitivity of GC cells. CONCLUSIONS: ZBED1 expression is up-regulated in GC cells. ZBED1 promotes proliferation and decreases the chemosensitivity of GC cells. ZBED1 may be a potential therapeutic target and predictive biomarker in gastric cancer. IJCEP
BACKGROUND:ZBED1 (zinc finger BED-type containing 1) is a transcription factor. However, its expression, role and clinical significance in cancer are unclear. The purpose of this study is to investigate the expression of ZBED1 and its effect on the proliferation of gastric cancer (GC). METHODS: Quantitative PCR was used to detect the mRNA level of ZBED1 in GC tissues and normal gastric tissues. Proliferation and colony formation assays were conducted when ZBED1 was expressed ectopically or silenced by constructed vectors. Moreover, chemotherapy-drug induced apoptosis rates were examined by flow cytometry when ZBED1 was expressed ectopically or silenced. RESULTS: The mRNA level of ZBED1 was significantly elevated in 10 out of 11 cases of GC tumor tissue specimens. Results of our analysis derived from a public clinical microarray database suggest that a high expression of ZBED1 predicts a poor outcome. ZBED1 promotes cell proliferation and colony formation. Moreover, ZBED1 decreased the chemosensitivity of GC cells. CONCLUSIONS:ZBED1 expression is up-regulated in GC cells. ZBED1 promotes proliferation and decreases the chemosensitivity of GC cells. ZBED1 may be a potential therapeutic target and predictive biomarker in gastric cancer. IJCEP
Authors: Elina A Radchenko; Anna Y Aksenova; Kirill V Volkov; Alexander A Shishkin; Youri I Pavlov; Sergei M Mirkin Journal: PNAS Nexus Date: 2022-06-08