Yufeng Xue1, Min Li1, Donghong Liu2, Qibing Zhu3, Huijun Chen3. 1. Department of Neurology, Tai Zhou First People's Hospital China. 2. Department of Laboratory, Tai Zhou First People's Hospital China. 3. Department of Pharmacy, Tai Zhou First People's Hospital China.
Abstract
BACKGROUND: This research was aimed to measure the expression of miR-9 in serum of acute ischemic stroke (AIS) patients and explore the role of miR-9 on OGD-induced neuronal damage. METHODS: In the present study, we measured the expression of miR-9 in serum of 65 AIS patients by real-time quantitative PCR (RT-qPCR) and the effect of miR-9 on oxygen-glucose deprivation (OGD)-induced neuronal injury was detected by CCK-8 in vitro. Western blot was used to measure the expression of protein. RESULTS: We found that the serum level of miR-9 in 65 AIS patients was significantly higher than that in control group (no-AIS), and was positively correlated with NIHSS score (r=0.627, P<0.001), infarct volume ((r=0.576, P<0.001), serum IL-8 (r=0.376, P=0.002), TNF-α (r=0.418, P<0.001), IL-6 (r=0.545, P<0.001), and IL-1β (r=0.592, P<0.001). miR-9 expression levels were upregulated in cultured neurons with OGD treatment. The downregulation of miR-9 significantly alleviated OGD-induced neuronal injury. Dual-luciferase reporter assay demonstrated that SIRT1 was a target gene of miR-9, and miR-9 negatively regulated SIRT 1 expression and positively regulated p65 expression. CONCLUSIONS: All in all, our data showed that downregulation of miR-9 protected neurons against OGD/R-induced injury by the SIRT1-mediated NF-kB pathway. IJCEP
BACKGROUND: This research was aimed to measure the expression of miR-9 in serum of acute ischemic stroke (AIS) patients and explore the role of miR-9 on OGD-induced neuronal damage. METHODS: In the present study, we measured the expression of miR-9 in serum of 65 AISpatients by real-time quantitative PCR (RT-qPCR) and the effect of miR-9 on oxygen-glucose deprivation (OGD)-induced neuronal injury was detected by CCK-8 in vitro. Western blot was used to measure the expression of protein. RESULTS: We found that the serum level of miR-9 in 65 AISpatients was significantly higher than that in control group (no-AIS), and was positively correlated with NIHSS score (r=0.627, P<0.001), infarct volume ((r=0.576, P<0.001), serum IL-8 (r=0.376, P=0.002), TNF-α (r=0.418, P<0.001), IL-6 (r=0.545, P<0.001), and IL-1β (r=0.592, P<0.001). miR-9 expression levels were upregulated in cultured neurons with OGD treatment. The downregulation of miR-9 significantly alleviated OGD-induced neuronal injury. Dual-luciferase reporter assay demonstrated that SIRT1 was a target gene of miR-9, and miR-9 negatively regulated SIRT 1 expression and positively regulated p65 expression. CONCLUSIONS: All in all, our data showed that downregulation of miR-9 protected neurons against OGD/R-induced injury by the SIRT1-mediated NF-kB pathway. IJCEP
Authors: Gerhard M Schratt; Fabian Tuebing; Elizabeth A Nigh; Christina G Kane; Mary E Sabatini; Michael Kiebler; Michael E Greenberg Journal: Nature Date: 2006-01-19 Impact factor: 49.962
Authors: Jakub S Nowak; Nila Roy Choudhury; Flavia de Lima Alves; Juri Rappsilber; Gracjan Michlewski Journal: Nat Commun Date: 2014-04-11 Impact factor: 14.919
Authors: Omayma O Abdelaleem; Olfat G Shaker; Mohamed M Mohamed; Tarek I Ahmed; Ahmed F Elkhateeb; Noha K Abdelghaffar; Naglaa A Ahmed; Abeer A Khalefa; Nada F Hemeda; Rania H Mahmoud Journal: Front Mol Biosci Date: 2022-02-14