Fuchan Wang1,2, Guangming Cao1, Qing Liu2, Xiulan Li2, Meiying Song3, Zhenyu Zhang1. 1. Department of Obstetrics and Gynecology, Beijing Chao-Yang Hospital, Capital Medical University Beijing, P. R. China. 2. Department of Obstetrics and Gynecology, Beijing You-An Hospital, Capital Medical University Beijing, P. R. China. 3. Department of Obstetrics and Gynecology, Beijing Fu-Xing Hospital, Capital Medical University Beijing, P. R. China.
Abstract
OBJECTIVE: Preeclampsia (PE) is a disorder of pregnancy that is associated with maternal and fetal mortality and morbidity. Abnormal placental Retinol-binding protein 4 (RBP4) expression has been found in cases of PE. This study aimed to further investigate the possible role of RBP4 in the pathophysiology of PE. STUDY DESIGN: RBP4 serum concentration was detected using enzyme-linked immunosorbent assays in eighteen patients with severe PE and twenty-five individuals with healthy pregnancies. JEG-3 cells were transiently transfected with a plasmid construct expressing RBP4 (pCMV-RBP4), an empty plasmid (p-CMV) or siRNA, and the MMP2 and MMP9 protein levels were analysed 48 h after transfection. MTT assays and transwell assays were used to explore the functional role of RBP4 in the proliferation and invasion of JEG-3 cells. RESULTS: RBP4 serum concentration in patients with severe PE was significantly lower than that in individuals with healthy pregnancies. RBP4 over-expression enhanced the invasion and proliferation of JEG-3 cells and increased MMP2 and MMP9 in JEG-3 cells. CONCLUSIONS: RBP4 plays an important role in the regulation of trophoblast invasion and migration and represents a possible underlying pathological and molecular mechanism of PE. IJCEP
OBJECTIVE: Preeclampsia (PE) is a disorder of pregnancy that is associated with maternal and fetal mortality and morbidity. Abnormal placental Retinol-binding protein 4 (RBP4) expression has been found in cases of PE. This study aimed to further investigate the possible role of RBP4 in the pathophysiology of PE. STUDY DESIGN:RBP4 serum concentration was detected using enzyme-linked immunosorbent assays in eighteen patients with severe PE and twenty-five individuals with healthy pregnancies. JEG-3 cells were transiently transfected with a plasmid construct expressing RBP4 (pCMV-RBP4), an empty plasmid (p-CMV) or siRNA, and the MMP2 and MMP9 protein levels were analysed 48 h after transfection. MTT assays and transwell assays were used to explore the functional role of RBP4 in the proliferation and invasion of JEG-3 cells. RESULTS:RBP4 serum concentration in patients with severe PE was significantly lower than that in individuals with healthy pregnancies. RBP4 over-expression enhanced the invasion and proliferation of JEG-3 cells and increased MMP2 and MMP9 in JEG-3 cells. CONCLUSIONS:RBP4 plays an important role in the regulation of trophoblast invasion and migration and represents a possible underlying pathological and molecular mechanism of PE. IJCEP
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