Yu Huang1, Nian Yang1. 1. Department of Gynecology and Obstetrics, Sichuan Academy of Medical Sciences, Sichuan Provincial People's Hospital Chengdu, Sichuan, China.
Abstract
BACKGROUND: Endometrial cancer (EC) is the fourth most common malignancy among women. Epithelial to mesenchymal transition (EMT) and invasion have been identified as central cellular processes in tumor progression and malignant transformation. The function and molecular basis of microRNA-20a-5p (miR-20a-5p) in the development of EC are poorly defined. METHODS: RT-qPCR assay was used to detect the levels of miR-20a-5p and signal transducer and activator of transcription 3 (STAT3) mRNA. Western blot assay was conducted to determine protein expression of STAT3, N-cadherin, Vimentin and E-cadherin. Cell invasive capacity was examined by transwell invasion assay. Bioinformatics analysis, luciferase reporter assay and RIP assay were performed to investigate the interaction between miR-20a-5p and STAT3 3'UTR. RESULTS: miR-20a-5p expression was strikingly reduced in EC tissues and cells. Functional analysis revealed that miR-20a-5p overexpression inhibited EMT and invasion of EC cells. Further exploration showed that miR-20a-5p inhibited STAT3 expression by direct interaction. Moreover, the knockdown of STAT3 suppressed EMT and invasion of EC cells. Additionally, the depletion of STAT3 weakened miR-20a-5p downregulation-induced cell EMT and invasion in EC. CONCLUSION: miR-20a-5p inhibited EMT and invasion of EC cells by targeting STAT3, highlighting the vital roles of miR-20a-5p and STAT3 in the metastasis and malignant transformation of EC. IJCEP
BACKGROUND:Endometrial cancer (EC) is the fourth most common malignancy among women. Epithelial to mesenchymal transition (EMT) and invasion have been identified as central cellular processes in tumor progression and malignant transformation. The function and molecular basis of microRNA-20a-5p (miR-20a-5p) in the development of EC are poorly defined. METHODS: RT-qPCR assay was used to detect the levels of miR-20a-5p and signal transducer and activator of transcription 3 (STAT3) mRNA. Western blot assay was conducted to determine protein expression of STAT3, N-cadherin, Vimentin and E-cadherin. Cell invasive capacity was examined by transwell invasion assay. Bioinformatics analysis, luciferase reporter assay and RIP assay were performed to investigate the interaction between miR-20a-5p and STAT3 3'UTR. RESULTS: miR-20a-5p expression was strikingly reduced in EC tissues and cells. Functional analysis revealed that miR-20a-5p overexpression inhibited EMT and invasion of EC cells. Further exploration showed that miR-20a-5p inhibited STAT3 expression by direct interaction. Moreover, the knockdown of STAT3 suppressed EMT and invasion of EC cells. Additionally, the depletion of STAT3 weakened miR-20a-5p downregulation-induced cell EMT and invasion in EC. CONCLUSION: miR-20a-5p inhibited EMT and invasion of EC cells by targeting STAT3, highlighting the vital roles of miR-20a-5p and STAT3 in the metastasis and malignant transformation of EC. IJCEP
Authors: Uksha Saini; Shan Naidu; Adam C ElNaggar; Hemant Kumar Bid; John J Wallbillich; Kristin Bixel; Chelsea Bolyard; Adrian A Suarez; Balveen Kaur; Periannan Kuppusamy; John Hays; Paul J Goodfellow; David E Cohn; Karuppaiyah Selvendiran Journal: Oncogene Date: 2016-06-13 Impact factor: 9.867
Authors: Jan Pencik; Michaela Schlederer; Wolfgang Gruber; Christine Unger; Steven M Walker; Athena Chalaris; Isabelle J Marié; Melanie R Hassler; Tahereh Javaheri; Osman Aksoy; Jaine K Blayney; Nicole Prutsch; Anna Skucha; Merima Herac; Oliver H Krämer; Peter Mazal; Florian Grebien; Gerda Egger; Valeria Poli; Wolfgang Mikulits; Robert Eferl; Harald Esterbauer; Richard Kennedy; Falko Fend; Marcus Scharpf; Martin Braun; Sven Perner; David E Levy; Tim Malcolm; Suzanne D Turner; Andrea Haitel; Martin Susani; Ali Moazzami; Stefan Rose-John; Fritz Aberger; Olaf Merkel; Richard Moriggl; Zoran Culig; Helmut Dolznig; Lukas Kenner Journal: Nat Commun Date: 2015-07-22 Impact factor: 14.919