| Literature DB >> 31945569 |
Baoguo Xu1, Jun Yuan2, Lin Wang3, Feng Lu4, Benxi Wei4, Roknul S M Azam4, Xiaofeng Ren5, Cunshan Zhou1, Haile Ma1, Bhesh Bhandari6.
Abstract
Effect of multi-frequency power ultrasound (MFPU) pretreatments on the degree of hydrolysis (DH) and mechanism of casein during alcalase enzymolysis was investigated. Results showed that MFPU pretreatment in tri-frequency 20/40/60 kHz mode significantly (p < 0.05) improved the DH value of casein. Variation of intrinsic fluorescence spectrum indicated the unfolding and degradation of casein occurred after MFPU pretreatment. Fourier transform infrared spectra showed that α-helix and β-sheet content of MFPU pretreated casein decreased, while β-turn and random coil content increased. Surface topography and nanostructures of caseins were found modified after MFPU pretreatments by the analysis of scanning electron microscopy (SEM) and atomic force microscopy (AFM). The SEM analysis also indicated that the enzymolysis residues of casein pretreated by MFPU were smaller than untreated samples. In conclusion, the MFPU can be used as an efficient pretreatment method to promote the enzymolysis of casein.Entities:
Keywords: AFM; Casein; Enzymolysis; Multi-frequency power ultrasound; SEM
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Year: 2019 PMID： 31945569 DOI： 10.1016/j.ultsonch.2019.104930
Source DB: PubMed Journal: Ultrason Sonochem ISSN： 1350-4177 Impact factor: 7.491