| Literature DB >> 31942825 |
Jeffery Ho1,2,3, Yun Kit Yeoh1,2,3, Nilakshi Barua1, Zigui Chen1,2,3, Grace Lui2,3,4, Sunny H Wong2,3,4, Xiao Yang1, Martin Cw Chan1, Paul Ks Chan1,2,3, Peter M Hawkey5, Margaret Ip1,2,3.
Abstract
In this review, we highlight the variations of gut resistome studies, which may preclude comparisons and translational interpretations. Of 22 included studies, a range of 12 to 2000 antibiotic resistance (AR) genes were profiled. Overall, studies defined a healthy gut resistome as subjects who had not taken antibiotics in the last three to 12 months prior to sampling. In studies with de novo assembly, AR genes were identified based on variable nucleotide or amino acid sequence similarities. Different marker genes were used for defining resistance to a given antibiotic class. Validation of phenotypic resistance in the laboratory is frequently lacking. Cryptic resistance, collateral sensitivity and the interaction with repressors or promotors were not investigated. International consensus is needed for selecting marker genes to define resistance to a given antibiotic class in addition to uniformity in phenotypic validation and bioinformatics pipelines.Entities:
Keywords: Resistome; antibiotic resistance; fecal microbiota transplantation; gut microbiota; meta-genomics
Year: 2020 PMID： 31942825 PMCID： PMC7524153 DOI： 10.1080/19490976.2019.1700755
Source DB: PubMed Journal: Gut Microbes ISSN： 1949-0976