Literature DB >> 31939189

Optogenetics: Rho GTPases Activated by Light in Living Macrophages.

Maren Hülsemann1,2, Polina V Verkhusha1, Peng Guo1,2,3, Veronika Miskolci4, Dianne Cox5,6, Louis Hodgson7,8.   

Abstract

Genetically encoded optogenetic tools are increasingly popular and useful for perturbing signaling pathways with high spatial and temporal resolution in living cells. Here, we show basic procedures employed to implement optogenetics of Rho GTPases in a macrophage cell line. Methods described here are generally applicable to other genetically encoded optogenetic tools utilizing the blue-green spectrum of light for activation, designed for specific proteins and enzymatic targets important for immune cell functions.

Entities:  

Keywords:  LOV2; Optogenetics; Photoactivatable proteins; Rho GTPases

Year:  2020        PMID： 31939189      PMCID： PMC7032845          DOI： 10.1007/978-1-0716-0247-8_24

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  23 in total

Review 1.  The evolution of flavin-binding photoreceptors: an ancient chromophore serving trendy blue-light sensors.

Authors:  Aba Losi; Wolfgang Gärtner
Journal:  Annu Rev Plant Biol       Date:  2011-11-15       Impact factor: 26.379

2.  Engineering extrinsic disorder to control protein activity in living cells.

Authors:  Onur Dagliyan; Miroslaw Tarnawski; Pei-Hsuan Chu; David Shirvanyants; Ilme Schlichting; Nikolay V Dokholyan; Klaus M Hahn
Journal:  Science       Date:  2016-12-16       Impact factor: 47.728

Review 3.  LOV to BLUF: flavoprotein contributions to the optogenetic toolkit.

Authors:  John M Christie; Jayde Gawthorne; Gillian Young; Niall J Fraser; Andrew J Roe
Journal:  Mol Plant       Date:  2012-03-19       Impact factor: 13.164

4.  Using Fluorescence Resonance Energy Transfer-Based Biosensors to Probe Rho GTPase Activation During Phagocytosis.

Authors:  Veronika Miskolci; Louis Hodgson; Dianne Cox
Journal:  Methods Mol Biol       Date:  2017

5.  A novel spatiotemporal RhoC activation pathway locally regulates cofilin activity at invadopodia.

Authors:  Jose Javier Bravo-Cordero; Matthew Oser; Xiaoming Chen; Robert Eddy; Louis Hodgson; John Condeelis
Journal:  Curr Biol       Date:  2011-04-07       Impact factor: 10.834

6.  Optical Tools To Study the Isoform-Specific Roles of Small GTPases in Immune Cells.

Authors:  Veronika Miskolci; Bin Wu; Yasmin Moshfegh; Dianne Cox; Louis Hodgson
Journal:  J Immunol       Date:  2016-03-07       Impact factor: 5.422

7.  Live cell imaging of RhoGTPase biosensors in tumor cells.

Authors:  Jose Javier Bravo-Cordero; Yasmin Moshfegh; John Condeelis; Louis Hodgson
Journal:  Methods Mol Biol       Date:  2013

8.  A new genetically encoded single-chain biosensor for Cdc42 based on FRET, useful for live-cell imaging.

Authors:  Samer Hanna; Veronika Miskolci; Dianne Cox; Louis Hodgson
Journal:  PLoS One       Date:  2014-05-05       Impact factor: 3.240

9.  A genetically encoded photoactivatable Rac controls the motility of living cells.

Authors:  Yi I Wu; Daniel Frey; Oana I Lungu; Angelika Jaehrig; Ilme Schlichting; Brian Kuhlman; Klaus M Hahn
Journal:  Nature       Date:  2009-08-19       Impact factor: 49.962

10.  LOVTRAP: an optogenetic system for photoinduced protein dissociation.

Authors:  Hui Wang; Marco Vilela; Andreas Winkler; Miroslaw Tarnawski; Ilme Schlichting; Hayretin Yumerefendi; Brian Kuhlman; Rihe Liu; Gaudenz Danuser; Klaus M Hahn
Journal:  Nat Methods       Date:  2016-07-18       Impact factor: 28.547
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