Huitao Zhu1, Nannan Sun2, Yan Wang2, Huichao Zhu3, Xiaolan Cai4, Xuezhong Li4. 1. Department of Otolaryngology, Weifang People's Hospital Weifang, China. 2. Department of Pathology, Qilu Hospital, Shandong University Jinan 250012, China. 3. Department of Mathematical Sciences, DePaul University Chicago, USA. 4. Department of Otolaryngology, Qilu Hospital, Shandong University Jinan 250012, China.
Abstract
BACKGROUND: This study was designed to explore the characteristics of inflammatory infiltration and tissue remodeling in the adjacent unaffected sinus mucosa of the polyp tissue (ANP). METHODS: Nasal polyps (NP) and ANP were obtained from 24 CRSwNP patients who received endoscopic sinus surgery. The frequency and distribution of Eosinophils, T lymphocytes (CD4+ T cells and CD8+ T cells), B lymphocytes, native macrophages, regulatory T cells and the total of inflammatory cells were detected by immunohistochemistry and hematoxylin-eosin stain. The thickness of the basal membrane was evaluated. RESULTS: Multivariate analysis of Variance (MANOVA) and F-tests were conducted for each independent variable between two groups. With test criterion alpha = 0.05, significant differences were observed between NP and ANP groups in terms of CD3 (F-Value = 10.47, P-value = 0.0120), CD4 (F-Value = 9.03, P-value = 0.0169), CD8 (F-Value = 17.03, P-value = 0.0033) and regulatory T cells (F-Value = 60.42, P-value <0.0001). Wilks' Lambda test (F-Value = 25.74, P-value = 0.1513) was conducted and no significant difference was observed between the NP group and the ANP group. The percentage of regulatory T cells in ANP was significantly higher than that in NP (3.7110±0.2395 vs 14.6300±1.8360). CONCLUSION: ANP and NP may be one disease entity. Treg cells have impacts on the morphology of the tissues and might be a key factor in the further development of ANP. IJCEP
BACKGROUND: This study was designed to explore the characteristics of inflammatory infiltration and tissue remodeling in the adjacent unaffected sinus mucosa of the polyp tissue (ANP). METHODS:Nasal polyps (NP) and ANP were obtained from 24 CRSwNP patients who received endoscopic sinus surgery. The frequency and distribution of Eosinophils, T lymphocytes (CD4+ T cells and CD8+ T cells), B lymphocytes, native macrophages, regulatory T cells and the total of inflammatory cells were detected by immunohistochemistry and hematoxylin-eosin stain. The thickness of the basal membrane was evaluated. RESULTS: Multivariate analysis of Variance (MANOVA) and F-tests were conducted for each independent variable between two groups. With test criterion alpha = 0.05, significant differences were observed between NP and ANP groups in terms of CD3 (F-Value = 10.47, P-value = 0.0120), CD4 (F-Value = 9.03, P-value = 0.0169), CD8 (F-Value = 17.03, P-value = 0.0033) and regulatory T cells (F-Value = 60.42, P-value <0.0001). Wilks' Lambda test (F-Value = 25.74, P-value = 0.1513) was conducted and no significant difference was observed between the NP group and the ANP group. The percentage of regulatory T cells in ANP was significantly higher than that in NP (3.7110±0.2395 vs 14.6300±1.8360). CONCLUSION: ANP and NP may be one disease entity. Treg cells have impacts on the morphology of the tissues and might be a key factor in the further development of ANP. IJCEP