Xing Chen1, Yingfang Zhou1. 1. Department of Obstetrics and Gynecology, Peking University First Hospital Beijing, China.
Abstract
OBJECTIVE: To observe the preventive effects of oral mucosal epithelial cells (OMECs) seeded on a decellularized human amniotic membrane (dHAM) in a rat model of intrauterine adhesion (IUA). METHODS: IUAs were established by mechanical endometrial scraping in model rats. Thirty-six Sprague-Dawley rats were divided into: IUA, dHAM transplantation, and dHAM+OMECs transplantation groups (12 in each group). The oral mucosae of rats were collected, and the OMECs were cultured on the dHAM. The dHAM and dHAM+OMECs were transplanted into left scraped uteri (right uteri as controls) in the dHAM and dHAM+OMECs transplantation groups. At 3, 7, 14, and 28 days after transplantation, the uterine samples underwent histological and immunohistochemical staining. RESULTS: After endometrial scraping, the endometria and uterine cavities were obliterated. The ratios of the fibrotic areas to the whole endometrium in the IUA and dHAM transplantation group were higher than in the control group (P<0.01). In the dHAM+OMECs transplantation group, the fibrotic area significantly decreased compared to the IUA group after 7, 14, and 28 days, and the dHAM transplantation group at 14 and 28 days (P<0.01). At 14 and 28 days after surgery in the dHAM+OMECs transplantation group, the number of endometrial glands and the endometrial thickness increased (P<0.01), and regenerated epithelia were observed. Immunohistochemical assays for cytokeratin 18 demonstrated that the newly-regenerated epithelium was endometrium. CONCLUSION: OMECs can enhance repair of damaged endometrium, and promise to be novel and effective in preventing IUAs. However, studies of OMECs in patients with IUA need further exploration. IJCEP
OBJECTIVE: To observe the preventive effects of oral mucosal epithelial cells (OMECs) seeded on a decellularized human amniotic membrane (dHAM) in a rat model of intrauterine adhesion (IUA). METHODS: IUAs were established by mechanical endometrial scraping in model rats. Thirty-six Sprague-Dawley rats were divided into: IUA, dHAM transplantation, and dHAM+OMECs transplantation groups (12 in each group). The oral mucosae of rats were collected, and the OMECs were cultured on the dHAM. The dHAM and dHAM+OMECs were transplanted into left scraped uteri (right uteri as controls) in the dHAM and dHAM+OMECs transplantation groups. At 3, 7, 14, and 28 days after transplantation, the uterine samples underwent histological and immunohistochemical staining. RESULTS: After endometrial scraping, the endometria and uterine cavities were obliterated. The ratios of the fibrotic areas to the whole endometrium in the IUA and dHAM transplantation group were higher than in the control group (P<0.01). In the dHAM+OMECs transplantation group, the fibrotic area significantly decreased compared to the IUA group after 7, 14, and 28 days, and the dHAM transplantation group at 14 and 28 days (P<0.01). At 14 and 28 days after surgery in the dHAM+OMECs transplantation group, the number of endometrial glands and the endometrial thickness increased (P<0.01), and regenerated epithelia were observed. Immunohistochemical assays for cytokeratin 18 demonstrated that the newly-regenerated epithelium was endometrium. CONCLUSION: OMECs can enhance repair of damaged endometrium, and promise to be novel and effective in preventing IUAs. However, studies of OMECs in patients with IUA need further exploration. IJCEP
Authors: Mohamed I Amer; Karim H I Abd-El-Maeboud; Ihab Abdelfatah; Fekrya Ahmad Salama; Al Said Abdallah Journal: J Minim Invasive Gynecol Date: 2010-06-23 Impact factor: 4.137