Non-invasive forced oscillometry to quantify respiratory mechanics in term neonates.

BACKGROUND: To determine normative data by forced oscillation technique (FOT) in non-sedated normal term neonates and test the hypothesis that infants with transient tachypnea of the newborn (TTN) have higher resistance (R) and lower reactance (X) on day 1.
METHODS: Healthy term infants (n = 138) and infants with TTN (n = 17) were evaluated on postnatal days 1 through 3 (NCT03346343). FOT was measured with a mask using a TremoFlo C-100 Airwave System™. R, X, and area under the reactance curve (AX) were measured at prime frequencies 7-41 Hz for 8 s.
RESULTS: In all, 86% of control infants had adequate measurements (coherence >0.8, CV < 0.25) on day 1. Infants with TTN had higher resistance at 13 Hz (TTN 32.5 cm H2O·s/L [95% CI 25.5-39.4]; controls 23.8 cm H2O·s/L [95% CI 22.2 to 25.3], P = 0.007) and lower reactance from 17 to 37 Hz (TTN -35.1 to -10.5; controls -26.3 to -6.1, P < 0.05). In healthy controls, lung mechanics were unchanged from days 1 to 3. In TTN, lung mechanics normalized on days 2 and 3.
CONCLUSIONS: FOT is feasible in neonates and distinguishes normal control infants from those with TTN on postnatal day 1. Oscillometry offers a non-invasive, longitudinal technique to assess lung mechanics in newborns.

Introduction

Pulmonary function testing (PFT) detects respiratory diseases and evaluates response to therapeutic intervention. Unfortunately, these measures are typically only available in older children and adults who are able to perform spirometry (1). Currently available PFT for neonates requires sedation with methods such as forced inhalation and exhalation or requires sophisticated and frequently cumbersome equipment such as the single occlusion and nitrogen washout techniques, limiting their widespread use outside of research centers (2, 3). Therefore, PFT techniques that can be performed non-invasively, reproducibly, and longitudinally at the bedside in neonates without these difficulties are desirable.

The forced oscillation technique (FOT) is a method in which a multi-frequency oscillatory signal is super-imposed on spontaneous tidal breathing to provide measurements of respiratory mechanics (4). FOT can be performed in less than half a minute, non-invasively, without sedation, and longitudinally (5), and usually takes only a few minutes for set-up time. FOT can measure total resistance (R), which relates to the impedance of signal through the respiratory system (e.g. the airways and tissues), and reactance (X), which corresponds to elasticity and inertia in the airway tree and tissues (5–7). The measures of R and X depend on the frequency of the transmitted oscillatory signal, with the medium range frequency from 5–40 Hz providing the greatest information about airway resistance and reactance; lower or higher frequencies measure tissue properties and acoustic wave propagation (6). The area under the reactance curve (AX) is a calculated value that reflects the frequency dependence of these changes (8). Hence, FOT is an attractive tool to quantify pulmonary function and track changes in disease trajectory. However, uptake of FOT in routine neonatal practice has been slow due to lack of reproducible normative neonatal values that distinguish health from disease as well as limited commercially available FOT equipment suitable for neonates.

Transient tachypnea of the newborn (TTN) is a frequently encountered neonatal respiratory disorder that is characterized by pulmonary edema resulting from delayed resorption and clearance of fetal alveolar fluid (9). Using nitrogen washout testing in combination with volume displacement plethysmography and an airway interrupter technique, it has been previously found that infants with TTN often have decreased compliance, in addition to other abnormalities of lung mechanics and function (10). As TTN is a common respiratory illness that usually resolves without therapeutic escalation, this disease was selected as the initial condition to evaluate the utility of FOT to detect disease and monitor improvement. Our objective was to determine feasibility and obtain normative data for FOT in non-sedated, term neonates without respiratory disease and to test the hypothesis that neonates with TTN have decreased X (which shares properties with compliance) and increased R compared to controls soon after birth.

Methods

Study Design and Participants

This was a single-center prospective observational cohort study evaluating the forced oscillation technique in healthy term infants and infants with TTN. Data were reported according to the Strengthening the Reporting of Observational Studies in Epidemiology (STROBE) reporting guidelines for cohort studies (www.strobe-statement.org). This study was approved by the Institutional Review Board at the University of Alabama at Birmingham (UAB), Birmingham, Alabama. The study is part of a project registered with Clinicaltrials.gov (NCT03346343) evaluating the FOT method in preterm and term infants. From January 1 through November 16, 2018, 175 infants born at UAB hospital were eligible and enrolled following informed consent from an available parent. Inclusion criteria for the control cohort included a gestational age ≥ 37 weeks and enrollment within 24 hours from birth; exclusion criteria included one or more of the following: receiving supplementary oxygen, signs of respiratory distress (defined as respiratory rate > 60/min, subcostal retractions, or nasal flaring), and congenital malformations. Inclusion criteria for the TTN cohort included a diagnosis of TTN (defined as tachypnea with maintained respiratory rate (RR) >60 breaths/min at 2 hours of age) and enrollment within 24h from birth; exclusion criteria included congenital malformations and a diagnosis of other causes of respiratory distress (e.g. respiratory distress syndrome or meconium aspiration syndrome) (11, 12). Tachypnea was defined as RR >60/min at the time of exam. Our previous evaluation of 488 term infants (13) indicated that although 50% of infants had RR >60 bpm in the first hour after birth, subsequent measurements were 50 bpm (SD 7). Hence, we anticipated that only about 5% of normal infants would have a RR>60 bpm at the time point of evaluation. We used the most recent charting for initial screening of infants, followed by study team evaluation for confirmation.

While most cases of TTN do not require escalation of support, infants in the TTN group were not tested if receiving endotracheal intubation or continuous positive airway pressure (CPAP), but testing was permissible once weaned from such respiratory support. Chest X-ray findings were used to substantiate the diagnosis of TTN but were not a prerequisite as not all infants required an X-ray per the clinical team. Gestational age was confirmed by earliest ultrasonographic measurements available through prenatal records. Patient characteristics were collected through chart review.

Materials and Procedures:

All enrolled infants were tested after enrollment by one of the authors on postnatal day 1, 2, and 3 (as available). Measurements were obtained using a TremoFlo C-100 Airwave System™ (THORASYS Thoracic Medical Systems Inc. Montreal, Quebec, Canada), custom fitted and calibrated (by the manufacturer) with a filter (Humid-Vent Filter Small A, Teleflex Medical, Ireland) and infant face mask (Portex infant, large). The device was calibrated daily. Calibration was performed by using a dynamic method that takes into account the shunt impedance through the filter. The mask is regarded as a static part of the respiratory system in this setup. The calibrated range of the device used in this study has been modified; however, the hardware is identical to the commercially available Tremoflo C-100 device. The dynamic calibration impedance range of the device used in this study is from 0 – 90 cmH2O/L/s. The factory calibration was checked daily by measuring a test load with a known impedance of 2 cmH2O/L/s, which is the standard reference for the TremoFlo System™. The instrument has been found to be linear in calibration (14). A fault in the system, including the dynamic calibration, would be detected by the daily check. The software used was the standard Tremoflo supplied by the manufacturer without any modifications. The mask is air cushioned and is compliant to fit snugly over the nose and mouth of the infant, hence minimizing the risk for leaks. If there was a leak at the relatively high impedances seen in infants, this would be quickly detected as the impedance would drop precipitously. The software also allows for post hoc inspection of the measurement time course and any measurement with a leak was discarded.

To perform a test, the device and mask were placed on the infant’s face covering the nose and slightly open mouth, while the infant was in the supine, “sniffing” position. Control infants were tested at the bedside in the mother’s room, in the well infant nursery, or in the neonatal intensive care unit. Infants in the TTN cohort were tested in the intensive care unit unless moved to the nursery. Initial testing occurred immediately after enrollment, and ensuing daily tests were then scheduled in accordance with parental preferences. Timing of testing was not standardized to occur only while asleep or after a feed, but instead reflected availability for evaluation, as would occur in common clinical scenarios. Infants undergoing testing in the intensive care unit had their vital signs continuously monitored for any adverse events, such as apnea, desaturations, or bradycardia. Infants tested in the nursery were monitored before and after testing.

Each FOT measurement was performed for 8 seconds using prime frequencies from 7 – 41 Hz for each test. Prime frequencies were used to prevent interference in measurements caused by harmonic amplifications of non-prime oscillatory signals. The frequency range from 7 – 41 Hz was based on data from previous studies, which demonstrated the greatest utility in measuring airway R and X with decreased amount of noise from respiratory testing (6, 8). On each testing day, up to 5 measurements were made per infant, with the mean results of at least 2 adequate measurements being used for analysis. Total time for set-up of equipment, preparing the patient, and measurement of the 5 tests was less than five minutes for each testing event. A measurement was considered adequate if there was an appropriate seal around the infant’s nose and mouth throughout the entire test, no vocalizations made during testing, no swallowing or obvious glottis closure during testing, and the coherence function value at each tested frequency was at least 0.80. The coherence function, which evaluates the amount of noise and abnormalities present in the measured signal, was calculated for all tested frequencies (15). The tested resistances must also have a coefficient of variation (CV) of < 25% among them at each frequency. These coherence and coefficient of variation thresholds for validity were based on our preliminary results and success rates in testing. Other studies have used coherence of at least 0.90 and CV of at least 15% (16); Data collected at time of testing included respiratory resistance (R) and reactance (X) at each prime frequency 7 – 41 Hz for each measurement. The area under the reactance curve (AX) across all frequencies was also obtained with testing. If patients had been discharged early, were unavailable for testing, or uncooperative with testing, that day’s tests were not included for analysis.

Statistical Analysis

We analyzed the changes in R, X, and AX for the 3 days’ data for control infants. Each day’s data were compared between the control and TTN groups (e.g., day 1 TTN compared to day 1 control). Averages and 95% confidence intervals were calculated for each day based on the individual patients’ mean data. Background and demographic data were also compared between the two groups. Categorical variables were compared by the Fisher’s exact test or χ2 test. Continuous variables were compared between TTN and control groups by two sample t-tests with 2 tails or Wilcoxon rank sum test if applicable. A generalized mixed model was used to compare the resistance and reactance at each frequency among the controls and between the two groups from days 1 through 3. P values of 0.05 or less were considered to indicate statistical significance. Statistical analysis was performed using SAS, version 9.4 (SAS Institute). Where data were missing or not obtained, the appropriate denominator was indicated in the tables.

To determine the required sample size for evaluating differences in infants with TTN versus controls, assuming initial measurements of lung reactance at 7 Hz would have an absolute difference of 50% (more negative) in infants with TTN compared to term controls, the minimum sample size required was 34 infants (17 per arm) assuming power of 80%, type I error rate of 5% and standard deviation of 0.5 of the mean (based on initial preliminary data in 6 infants during initial testing of the device). To improve the power of the study, the entire sample of control infants was compared to the TTN group. However, for development of normative data in normal control infants, a larger sample size was required to reduce the width of confidence intervals. With low sample size, confidence intervals are wide, and with increasing sample size, the width of the confidence intervals get narrower and after 50–75 subjects, additional reductions are minimal even with doubling or tripling the sample size (17). As normative data and variability in measurements by FOT in normal term infants have not been determined in prior literature, and the feasibility and rate of drop-outs could not be predicted in advance, a sample size of approximately 125 control infants (in excess of the 50–75 needed, assuming a 50% failure rate) was empirically chosen and considered sufficient to generate reference data in this study.

Results

During the study period from January to November 2018, we enrolled 175 infants, with 158 in the control cohort and 17 in the TTN cohort. Of the 158 infants enrolled in the control group, 138 (87%) successfully completed at least one set of adequate tests; all 17 (100%) in the TTN cohort had at least one day of adequate tests. 96% of infants in the control cohort and 29% of infants in the TTN cohort were enrolled from the well infant nursery. Enrollment and attainment of FOT results over the three days for control and TTN infants are shown in Figure 1. Among the 138 healthy control infants who had FOT testing performed, 86% (118 out of 137) had adequate tests on day 1, 75% (90/120) had adequate tests on day 2, and 61% (33/54) had adequate tests on day 3. Most of these unavailable infants on days 2 and 3 were discharged home as is routine for normal infants, prior to completion of testing. Infants who remained for all three days were more likely to be delivered via Cesarean section, born to previously nulliparous mothers, or had an underlying reason to be kept (e.g. maternal illness). The increase in poor quality tests among controls coincided with an increase in infant agitation with testing – infants on days 2 and 3 were more likely to be awake during testing or vocalized during the tests. We attempted to only obtain measurements when most convenient for the parents and families, but this was not always optimal as some infants were tested awake or before feeds when they were more likely to develop agitation, rather than when they were sleeping or resting after feeds. As mentioned previously we used a coherence of 0.8 and CV of 25%. 28% of our data points were above the previously used CV of 15%, and 35% of our data were above the previously used coherence of 0.9. We did not see any sudden changes in breathing pattern over the 8 seconds of each measurement, and the mask was removed immediately after the measurement was complete and reapplied at least 30 seconds later for the next measurement. No adverse events (e.g. bradycardia, apnea, or desaturations) associated with testing were noted with any of the studied infants.

Figure 1:

Enrollment diagram of study participants. Reasons for missing tests are infant agitation during testing, infant unavailability (due to discharge, unavailability during testing times, or need for nasal CPAP for TTN infants), or parental refusal. Participants who were unavailable for testing were excluded from the final adequate test result numbers.

Demographic and clinical variables are described in Table 1. As anticipated, infants with TTN were more often delivered by Cesarean section, born at a lower gestational age, received lower 1- and 5-minute Apgar scores, had higher respiratory rates at time of enrollment, and required additional respiratory support. Five out of the 17 infants in the TTN cohort received supplementary respiratory support beyond oxygen administration (i.e. nasal cannula or CPAP) at some point during their hospitalization. Other demographic and background variables did not differ between the two groups.

Table 1:

Baseline demographic and clinical characteristics of study participants

	Controls (n = 138)	TTN (n = 17)	P value[b]
Race, n (%)			0.11
African American	70 (51)	13 (76)
Caucasian	59 (42)	3 (18)
Other	9 (7)	1 (6)
Male Sex, n (%)	69 (50)	7 (41)	0.49
Vaginal Delivery, n (%)	108 (78)	6 (35)	<0.001
Gestational Age, weeks, Median (IQR)	39 2/7 (38 3/7 – 40)	37 4/7 (36 6/7 – 38 6/7)	0.001
Birth Weight, g Mean ± SD	3258 ± 444	3215 ± 755	0.41
Length, cm Mean ± SD	50.1 ± 2	48.8 ± 3	0.05
1 min Apgar, Median (IQR)	8 (8 – 9)	7 (6 – 8)	0.001
5 min Apgar, Median (IQR)	9 (8 – 9)	8 (8 – 9)	<0.001
SGA, n (%)	11 (8)	2 (12)	0.64
LGA, n (%)	5 (4)	2 (12)	0.17
Need for Respiratory Support, n (%)[a]	0 (0)	15 (88)	N/A
Respiratory Rate at Enrollment, breaths per minute, Median (IQR)	44 (40 – 49)	70 (66–76)	<0.001

Abbreviations: IQR, interquartile range.

Respiratory support defined as a need for supplemental oxygen or continuous positive airway pressure.

P values were determined by Fisher’s exact test or χ2 tests for categorical variables and two sided t-tests or Wilcoxon rank sum tests for continuous variables.

Mean resistance and reactance measurements for the control infants over the first three days are shown in Figure 2. R on day 1 ranged from 10.5 to 31.2 cmH2O·s/L, while X ranged from −46.6 to −6.1 cmH2O·s/L. On each day, there was an overall trend toward decreasing resistance with increasing frequency. Reactance also steadily increased (became less negative) with increasing frequency. The largest differential from resistance to reactance was at the low frequencies, specifically 7 Hz. Analysis of data of control infants who were enrolled in all three days indicated that infants available on day 3 differed in characteristics from the initial study population. These infants were more likely to have been born via Caesarean section (48% vs 22%, P <0.001) or had other clinical reasons to remain hospitalized (e.g. neonatal abstinence syndrome or hyperbilirubinemia) (12% vs 6%, P<0.001).

Figure 2:

Respiratory Resistance (R) and Reactance (X) on day 1 (n=118), day 2 (n = 75) and day 3 (n =33) for healthy term infants. Error bars represent 95% CI.

R on day 1 in TTN infants ranged from 12.9 to 35.6 cmH2O·s/L, and X ranged from 49.4 to −10.2 cmH2O·s/L. Like the controls, the TTN infants had higher resistance at lower frequencies that decreased with higher frequency measurements, and reactance was more negative at low frequencies and approached 0 at the higher tested frequencies. Comparison of resistance and reactance between control and TTN infants for each day is shown in Figure 3. Resistance was higher in TTN patients on day 1 with a statistically significant difference at 13 Hz with TTN at 32.5 cmH2O·s/L (95% CI 25.5–39.4) versus controls at 23.8 cmH2O·s/L (95% CI 22.2–25.3), p = 0.007. Reactance was also lower (more negative) in infants with TTN on day 1 compared to controls with significant differences from 17 through 37 Hz, (all p < 0.02). ROC curves were generated at each reportedly significant frequency for both R and X on day 1, and the Area Under the Curve (AUC) of the ROC curves estimated (Table 2). The measured differences in resistance and reactance between TTN and controls were no longer present on days 2 and 3 with no statistically significant differences in R and X at any frequency. This coincided with clinical improvement also seen in the TTN infants on those days. The median respiratory rates for the TTN cohort decreased from 70 breaths per minute (IQR 66–76) on day 1 to 53 (IQR 48–60) on day 2, and 52 (IQR 43–67) on day 3. Median respiratory rates for controls did not significantly differ among the three days and ranged from 44 (IQR 40–49) on day 1, 44 (IQR 40–50) on day 2, and 42 (IQR 40–46) on day 3. Respiratory rates significantly differed between the TTN and control cohorts on day 1 (p < 0.001) and on day 2 (p < 0.001). Respiratory rates on day 3 were not significantly different between the two groups.

Figure 3:

Respiratory Resistance (R) and Reactance (X) on (a) day 1,(b) day 2, and (c) day 3 for control (day 1 n=118; day 2 n=75, day 3 n= 33) and TTN (day 1 n=13; day 2 n=13, day 3 n=6) infants. Error bars represent 95% CI. * represent measurements in which the difference between TTN and controls are P < 0.05 as determined by the mixed model.

Table 2:

Area under the ROC curve (AUC of ROC) comparison for resistance (R) and reactance (X) for TTN (versus control) infants on day 1 at each tested frequency.

Frequency (Hz)	AUC of ROC for Resistance (R)	P	AUC of ROC for Reactance (X)	P
7	0.57	0.28	0.56	0.38
11	0.61	0.06	0.60	0.08
13	0.71	0.006	0.64	0.03
17	0.70	0.08	0.68	0.009
19	0.70	0.05	0.70	0.006
23	0.67	0.36	0.69	0.003
29	0.59	0.88	0.71	0.006
31	0.3	0.36	0.65	0.03
37	0.49	0.65	0.65	0.007
41	0.61	0.56	0.63	0.04

The area under the reactance curve (AX) was calculated across all tested frequencies for each group on each day. AX ranged from 624 to 765 cmH2O/L for term infants from day 1 to day 3. There was no statistically significant change in AX among control infants over days 1 through 3. Mean AX was not significantly higher on day 1 in the TTN group, 834 cmH2O/L, compared to controls, 624 cmH2O/L, p= 0.08. There were once again no statistically significant differences in AX between TTN and control infants on days 2 and 3. Figure 4 shows comparisons of AX between the two groups.

Figure 4:

Area under the reactance curve measurements for enrolled infants on days 1 through 3.

Resistance and Reactance were modeled by Generalized Linear Mixed Models (GLIMMIX) for each frequency (7, 13, 17, 19, 23, 29, 31, 37, 41) taking birth weight, length, and sex of term infants into account. As birth weight and length were highly correlated (collinear), we generated separate models using birth weight and length (Supplemental Tables). However, birth weight and length were not strongly correlated with lung mechanics within the relatively narrow range of birth weight and length evaluated in this study. We also modeled AX by birth length and sex (Supplemental Tables).

Discussion

We performed FOT using a modified commercially available device in a cohort of spontaneously breathing, non-sedated, healthy term neonates on days 1, 2, and 3, and determined normative data for healthy neonates on the first postnatal day. We were able to follow some of these infants over time and evaluate respiratory mechanics longitudinally. This FOT data was also compared to infants with TTN and demonstrated feasibility in differentiating health from disease. As disease improved, the changes in respiratory mechanics found with oscillometry also improved and matched the normative data. Additionally, although respiratory rates still differed between the two groups on day 2, resistance and reactance no longer significantly differed, suggesting that respiratory rates alone cannot explain the differences detected in R and X on day 1. Previous FOT studies have used a very limited range of non-prime frequencies, which may not offer the same information on underlying respiratory mechanics in the neonatal population, or used custom built FOT devices (15, 18). Additionally, other neonatal FOT studies have used respiratory pauses by evoking the Hering-Breuer inflation reflex during oscillometry (19, 20) rather than evaluate FOT during quiet breathing, have evaluated only sedated infants (21), or evaluated premature neonates (22).

FOT in healthy term infants demonstrated small changes in resistance and reactance from day 1 through 3 at some frequencies. These results may indicate that even in healthy neonates without clinical respiratory disease, there may be small changes in respiratory mechanics as they continue to transition to extra-uterine life, or these differences may be due to flow-dependent changes in breathing patterns among infants from day 1 to 3. Resistance was higher across all three days in normal infants at lower frequencies. Reactance was also lower at the lower frequencies. This frequency dependence is commonly observed in forced oscillometry measurements and may be amplified by airway obstruction (23). The resistance and reactance curves generated are similar to that found in the study by Nowowiejska et al in children aged 3–18 (24). However, the absolute resistance in our control infants is about 4 times higher than that of the older children; similarly, the reactance is lower (more negative) by about 4 times that of the older children. This difference in resistance is similar to the findings by Gochicoa et al.(25) who measured airway resistance by the interrupter technique and compared results in neonates and children. Interestingly, mean R was lower than reported by Gray et al., (26) who tested unsedated infants at 6 to 10 weeks of age. Mean R values were similar in magnitude to those reported by Hantos et al. (18) who had previously tested a small subset of neonates in the first three days of life.

As anticipated, infants with TTN were more likely to have been born via Cesarean section, be of a younger gestational age, require more respiratory support, have lower Apgar scores, and have higher respiratory rates at enrollment, compared to the control group. These are all known risk factors for infants who develop TTN (9). Otherwise, demographics between the groups did not differ. When compared to healthy term infants on day 1, TTN infants had decreased reactance with statistically significant differences from 11 to 37 Hz suggesting reduced compliance. The study by Sandberg et al, showed similar changes of reduced compliance in infants with TTN (10). Lung edema is preferentially around airways and blood vessels in the lung interstitium (27), and such accumulation of interstitial fluid may not only reduce compliance and may also cause airway compression, increasing airway resistance. Previously, it had been shown that reactance may be more specific than resistance in assessing bronchial obstruction in children (24), so airway edema may also possibly contribute to the finding of decreased reactance in TTN infants. Infants with TTN also had increased resistance, specifically at 13 Hz, suggesting increased airway and viscous resistance, which may be due to airway compression. The changes in R and X found on day 1 in TTN infants as compared to controls were not seen on days 2 and 3, indicating that as the clinical disease of TTN improves, FOT quantification of respiratory mechanics also normalizes. However, FOT as performed in this study offers a non-invasive method to obtain information about respiratory mechanics, which can be performed longitudinally, and differentiate disease from healthy states.

There were a few limitations to our study. One of the main limitations was the reduction in number of measurements from day 1 to 3. Early discharge from the hospital limited the number of patients who could be tested sequentially. This potentially provides a source of bias in infants who remained in the hospital on day 3 for testing. Additionally, infants who remained hospitalized by day 3 differed in method of delivery and clinical reasons for prolonged admission from the total population initially enrolled. However, despite the change in the number of tests obtained, the results from day 1 to 3 in controls differed only minimally. Furthermore, even though the test is not invasive and only requires an 8 second interval to perform, the test requires a baseline level of cooperation from the infant. One cannot test the infant if they are crying or vocalizing. Our study had a number of infants who could not be tested while sleeping or in the quiet fed state, but were crying or otherwise agitated during testing on days 2 and 3. Testing should be performed by someone who is experienced in testing with neonatal patients to overcome these limitations. It must be noted that these figures are comparable or better than that of spirometry in preschool children of whom only 80% were able to produce at least one acceptable spirometry effort, with 54% able to repeat similar efforts (28) and acceptable and repeatable spirometry could be obtained in only 42% of 4–5 year olds (29). As mentioned previously, a coherence of at least 0.90 and coefficient of variation (CV) of at least 15% in three replicate measures provided the most accurate estimates in prior studies (16). However, others have shown that a coherence of at least 0.6 only at 5 Hz would provide adequate measurements (30). Our study elected to use a threshold for coherence of 0.8 and CV of 25% for validity based on our preliminary measurements and success rates, and even with these increased measures of validity, only about a third of the total measurements were outside of the threshold. Based on the results obtained from this study, and practical issues in testing, we provided feedback to THORASYS Thoracic Medical Systems Inc, who have since developed a new model, the Tremoflo N-100 “Neo” Airwave Oscillometry device, which has a flexible connecting element and a button to initiate measurement close to the mask, which should provide similar data but be easier to use in newborn infants.

In adults, oscillometry testing in supine versus sitting has been shown to provide different resistance-flow gradients based on body position (31). All infants tested were in the supine position and did not have a respiratory support device. It is possible that some infants had upper airway narrowing during supine testing, but this is difficult to determine or quantify, and it is possible that pulmonary mechanics measurements would be different if infants were positioned prone or on their side. Infants with TTN who qualified for testing did not require respiratory support above an oxygen hood or an incubator environment servo-controlled with oxygen. Testing of infants who were intubated, on CPAP, or nasal cannula would have required a different interface and technique, which was outside the scope of our study. Previous studies have suggested using a “head generator technique” to reduce upper airway resistance and artifact (32). However, nasal passages and upper airways are dominant areas of resistance in infants, such that it may be important to include the upper airways during respiratory testing in neonates (33). It is also possible that changes in upper airway resistances may have occurred or that total measurement of the infant’s tidal volume may have been lost due to the use of the infant face mask and filter applied to the device. This could potentially provide different R and X measurements when compared to alternative methods of FOT testing, such as performed by Hantos et al (18). However, because all infants in our study were tested in the same manner (i.e. using the Tremoflo™ device with mask and filter), results within our study and those replicated using the same technique should provide consistent results and similar measurements of R and X. It is certain that the use of the mask plus filter and the FOT device adds to the dead space, which may be a considerable percentage of the infant’s tidal volume. We did not see any sudden changes in breathing pattern over the 8 seconds of each measurement. We did not separately account for dead space as a percentage of the infants’ tidal volume, which would vary from infant to infant. Although higher respiratory rate in neonates may reduce the signal to noise ratio, this will be applicable to future studies using FOT in a comparable age group. Finally, we did not specifically evaluate lung mechanics during inspiration versus expiration, although some differences are likely. We noted that birth weight and length were not strongly correlated with lung mechanics within the relatively narrow range of birth weight and length noted in this study. It is likely that evaluation of older and larger infants or smaller preterm infants will require considerations of anthropometry.

In conclusion, this study provides normative non-invasive FOT data in non-sedated healthy term neonates on the first postnatal day. FOT can be performed longitudinally and reproducibly in this patient population. Our study demonstrates that testing is not only feasible in infants using non-invasive methods using a slightly modified commercially available device, but that differences in respiratory mechanics can be detected when investigating clinical disease. Additionally, improvements in respiratory mechanics coincide with resolution of the disease. The data support our hypothesis that TTN infants have lower reactance and higher resistance compared to healthy term infants on day 1. FOT presents a promising opportunity to longitudinally assess lung disease in neonates. Further studies in additional neonatal lung diseases such as RDS, BPD, and meconium aspiration syndrome may prove valuable to monitor disease trajectory and response to therapy.