Chong Liu1, Zhonghua Shang1, Yu Ma2, Jianfang Ma1, Jian Song1. 1. Department of General Surgery, Second Hospital of Shanxi Medical University Taiyuan, Shanxi, China. 2. Department of General Surgery, Tai Yuan City Central Hospital Taiyuan, Shanxi, China.
Abstract
BACKGROUND: Long non-coding RNA (lncRNA) Hox transcript antisense intergenic RNA (HOTAIR) has been reported to play an important role in hepatocellular carcinoma (HCC) progression. Although there is evidence on HOTAIR being associated with HCC progression, the underlying mechanism remains to be further clarified. METHODS: HOTAIR, miR-214-3p and FLOT1 expression were measured by quantitative real-time PCR or western blot. Cell proliferation, migration, and invasion were displayed by transwell assay, MTT, and colony formation assay. The relationship between HOTAIR, miR-214-3p, and FLOT1 was investigated by luciferase reporter assay. Tumor suppressive effect of HOTAIR was displayed by HepG2 xenografting to nude mice. RESULTS: HOTAIR and FLOT1 expression were significantly up-regulated, whereas miR-214-3p was obviously down-regulated. Also, down-regulation of HOTAIR and FLOT1 as well as up-regulation of miR-214-3p inhibited cell proliferation, invasion, and migration. Intriguingly, the effects of miR-214-3p overexpression on HCC cells were rescued by high expression of HOTAIR. Otherwise, HOTAIR regulated FLOT1 expression through targeting miR-214-3p in HCC cells. Simultaneously, low HOTAIR expression inhibited FLOT1 expression by up-regulating miR-214-3p, which suppressed tumor growth in vivo. CONCLUSION: HOTAIR expression indirectly regulated FLOT1 expression through endogenous competition with miR-214-3p. HOTAIR/miR-214-3p/FLOT1 axis affected the cell proliferation, migration, and invasion of HCC. IJCEP
BACKGROUND: Long non-coding RNA (lncRNA) Hox transcript antisense intergenic RNA (HOTAIR) has been reported to play an important role in hepatocellular carcinoma (HCC) progression. Although there is evidence on HOTAIR being associated with HCC progression, the underlying mechanism remains to be further clarified. METHODS:HOTAIR, miR-214-3p and FLOT1 expression were measured by quantitative real-time PCR or western blot. Cell proliferation, migration, and invasion were displayed by transwell assay, MTT, and colony formation assay. The relationship between HOTAIR, miR-214-3p, and FLOT1 was investigated by luciferase reporter assay. Tumor suppressive effect of HOTAIR was displayed by HepG2 xenografting to nude mice. RESULTS:HOTAIR and FLOT1 expression were significantly up-regulated, whereas miR-214-3p was obviously down-regulated. Also, down-regulation of HOTAIR and FLOT1 as well as up-regulation of miR-214-3p inhibited cell proliferation, invasion, and migration. Intriguingly, the effects of miR-214-3p overexpression on HCC cells were rescued by high expression of HOTAIR. Otherwise, HOTAIR regulated FLOT1 expression through targeting miR-214-3p in HCC cells. Simultaneously, low HOTAIR expression inhibited FLOT1 expression by up-regulating miR-214-3p, which suppressed tumor growth in vivo. CONCLUSION:HOTAIR expression indirectly regulated FLOT1 expression through endogenous competition with miR-214-3p. HOTAIR/miR-214-3p/FLOT1 axis affected the cell proliferation, migration, and invasion of HCC. IJCEP
Authors: Bal Krishna Chand Thakuri; Jinyu Zhang; Juan Zhao; Lam N Nguyen; Lam N T Nguyen; Sushant Khanal; Dechao Cao; Xindi Dang; Madison Schank; Xiao Y Wu; Zheng D Morrison; Mohamed El Gazzar; Zhengke Li; Yong Jiang; Shunbin Ning; Ling Wang; Jonathan P Moorman; Zhi Q Yao Journal: Sci Rep Date: 2020-12-16 Impact factor: 4.379
Authors: Jinyu Zhang; Bal Krishna Chand Thakuri; Juan Zhao; Lam N Nguyen; Lam N T Nguyen; Dechao Cao; Xindi Dang; Sushant Khanal; Madison Schank; Zeyuan Lu; Xiao Y Wu; Zheng D Morrison; Mohamed El Gazzar; Zhengke Li; Yong Jiang; Shunbin Ning; Ling Wang; Jonathan P Moorman; Zhi Q Yao Journal: AIDS Date: 2020-12-01 Impact factor: 4.632