Literature DB >> 3193361

A gene-cloning system for Kluyveromyces lactis and isolation of a chromosomal gene required for killer toxin production.

X J Chen1, M Wésolowski-Louvel, C Tanguy-Rougeau, M M Bianchi, L Fabiani, M Saliola, C Falcone, L Frontali, H Fukuhara.   

Abstract

A transformation system derived from the circular plasmid pKD1 has been developed for Kluyveromyces lactis. The principle is essentially equivalent to that of the 2 microns/Saccharomyces cerevisiae transformation system. The main features of the system are presented. Using a pKD1-based DNA bank of K. lactis, the KEX1 gene involved in the killer system was isolated by complementation.

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Year:  1988        PMID: 3193361     DOI: 10.1002/jobm.3620280402

Source DB:  PubMed          Journal:  J Basic Microbiol        ISSN: 0233-111X            Impact factor:   2.281


  7 in total

Review 1.  Yeast killer systems.

Authors:  W Magliani; S Conti; M Gerloni; D Bertolotti; L Polonelli
Journal:  Clin Microbiol Rev       Date:  1997-07       Impact factor: 26.132

2.  Transformation of Kluyveromyces lactis by Electroporation.

Authors:  M Sánchez; F J Iglesias; C Santamaría; A Domínguez
Journal:  Appl Environ Microbiol       Date:  1993-07       Impact factor: 4.792

3.  Molecular cloning of the Golgi apparatus uridine diphosphate-N-acetylglucosamine transporter from Kluyveromyces lactis.

Authors:  C Abeijon; P W Robbins; C B Hirschberg
Journal:  Proc Natl Acad Sci U S A       Date:  1996-06-11       Impact factor: 11.205

4.  The mitochondrial genome integrity gene, MGI1, of Kluyveromyces lactis encodes the beta-subunit of F1-ATPase.

Authors:  X J Chen; G D Clark-Walker
Journal:  Genetics       Date:  1996-12       Impact factor: 4.562

Review 5.  Deoxyribonucleic acid plasmids in yeasts.

Authors:  F C Volkert; D W Wilson; J R Broach
Journal:  Microbiol Rev       Date:  1989-09

6.  Mammalian Golgi apparatus UDP-N-acetylglucosamine transporter: molecular cloning by phenotypic correction of a yeast mutant.

Authors:  E Guillen; C Abeijon; C B Hirschberg
Journal:  Proc Natl Acad Sci U S A       Date:  1998-07-07       Impact factor: 11.205

7.  Specific mutations in alpha- and gamma-subunits of F1-ATPase affect mitochondrial genome integrity in the petite-negative yeast Kluyveromyces lactis.

Authors:  X J Chen; G D Clark-Walker
Journal:  EMBO J       Date:  1995-07-03       Impact factor: 11.598

  7 in total

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