Hao Lu1, Xinzheng Dai1, Xu Li1, Yu Sun1, Yangjuan Gao2, Chuanyong Zhang1. 1. Department of Liver Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210000, China. 2. Department of Radiology, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210000, China.
Abstract
BACKGROUND: This study aimed to investigate the mechanism of galectin (Gal)-1 of regulating Treg/Th17 in pathogenesis of acute rejection after liver transplantation in rat. METHODS: Mononuclear cells were induced to immature dendritic cells (imDCs), which were transfected with or without NF-κB/RelB. Western Blot was performed to detect the expression of NF-κB/RelB. the expression of CD11c, CD45RB, CD80 and MHC II were detected by flow cytometry. Enzyme-linked immunosorbent assay (ELISA) was employed to detect cytokines IL-27 and TGF-β. Lewis and dark agouti (DA) rats were generally anaesthetized by isoflurane inhalation to establish liver transplant models. RESULTS: We demonstrate that Gal-1 disturbs maturation of imDCs by downregulating NF-κB/RelB expression, and Gal-1 negatively controls CD4+ proliferation though IL-27 pathway. CONCLUSIONS: In aggregate, Gal-1 promotes Treg differentiation in CD4+ T cells though NF-κB/RelB-IL-27 pathway. These findings suggest a new therapeutic target to mediate Treg population. 2019 Annals of Translational Medicine. All rights reserved.
BACKGROUND: This study aimed to investigate the mechanism of galectin (Gal)-1 of regulating Treg/Th17 in pathogenesis of acute rejection after liver transplantation in rat. METHODS: Mononuclear cells were induced to immature dendritic cells (imDCs), which were transfected with or without NF-κB/RelB. Western Blot was performed to detect the expression of NF-κB/RelB. the expression of CD11c, CD45RB, CD80 and MHC II were detected by flow cytometry. Enzyme-linked immunosorbent assay (ELISA) was employed to detect cytokines IL-27 and TGF-β. Lewis and dark agouti (DA) rats were generally anaesthetized by isoflurane inhalation to establish liver transplant models. RESULTS: We demonstrate that Gal-1 disturbs maturation of imDCs by downregulating NF-κB/RelB expression, and Gal-1 negatively controls CD4+ proliferation though IL-27 pathway. CONCLUSIONS: In aggregate, Gal-1 promotes Treg differentiation in CD4+ T cells though NF-κB/RelB-IL-27 pathway. These findings suggest a new therapeutic target to mediate Treg population. 2019 Annals of Translational Medicine. All rights reserved.
Authors: M J García; F Jurado; D San Segundo; M López-Hoyos; P Iruzubieta; S Llerena; F Casafont; M Arias; Á Puente; J Crespo; E Fábrega Journal: Transplant Proc Date: 2015 Jan-Feb Impact factor: 1.066
Authors: Magdalena Huber; Vera Steinwald; Anna Guralnik; Anne Brüstle; Peter Kleemann; Christine Rosenplänter; Thomas Decker; Michael Lohoff Journal: Int Immunol Date: 2007-12-21 Impact factor: 4.823
Authors: Aisling O'Hara Hall; Daniel P Beiting; Cristina Tato; Beena John; Guillaume Oldenhove; Claudia Gonzalez Lombana; Gretchen Harms Pritchard; Jonathan S Silver; Nicolas Bouladoux; Jason S Stumhofer; Tajie H Harris; John Grainger; Elia D Tait Wojno; Sagie Wagage; David S Roos; Philip Scott; Laurence A Turka; Sara Cherry; Steven L Reiner; Daniel Cua; Yasmine Belkaid; M Merle Elloso; Christopher A Hunter Journal: Immunity Date: 2012-09-13 Impact factor: 31.745
Authors: Cecilia Svanberg; Sofia Nyström; Melissa Govender; Pradyot Bhattacharya; Karlhans F Che; Rada Ellegård; Esaki M Shankar; Marie Larsson Journal: Front Immunol Date: 2022-08-10 Impact factor: 8.786